Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,134,823 G→A *231* (TAG→TAA yghT → putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,8230GA100.0% 142.4 / NA 46*231* (TAG→TAAyghTputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

ACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAT  >  NC_000913/3134703‑3134949
                                                                                                                        |                                                                                                                              
acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                             <  2:24372/139‑1 (MQ=255)
acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                             <  2:11794/139‑1 (MQ=255)
acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                             <  2:1101892/139‑1 (MQ=255)
acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                             >  1:24372/1‑139 (MQ=255)
            tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                 >  2:533709/1‑139 (MQ=255)
                                      aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                         <  2:329897/137‑1 (MQ=255)
                                      aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                         >  1:329897/1‑137 (MQ=255)
                                         ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                    <  1:1370335/139‑1 (MQ=255)
                                           aaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                                  >  2:767777/1‑139 (MQ=255)
                                            aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAg                                                                                     >  2:290366/1‑119 (MQ=255)
                                            aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAg                                                                                     <  1:290366/119‑1 (MQ=255)
                                            aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCaa                                                                 >  1:997128/1‑139 (MQ=255)
                                                        aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                     <  1:533709/139‑1 (MQ=255)
                                                                 tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                    >  2:328692/1‑99 (MQ=255)
                                                                 tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                    <  1:328692/99‑1 (MQ=255)
                                                                 tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                               >  1:720771/1‑120 (MQ=255)
                                                                 tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                               <  2:720771/120‑1 (MQ=255)
                                                                  ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                              <  2:1059227/88‑1 (MQ=255)
                                                                  ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                              >  1:1059227/1‑88 (MQ=255)
                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                              >  2:1268831/1‑51 (MQ=255)
                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATc                                                                                                                              <  1:1268831/51‑1 (MQ=255)
                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                      >  2:1281745/1‑139 (MQ=255)
                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                      >  2:1328518/1‑139 (MQ=255)
                                                                               aaaGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTg                              >  2:209252/1‑139 (MQ=255)
                                                                                 aGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgcc                            <  1:1281745/139‑1 (MQ=255)
                                                                                  gCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                           <  1:1328518/139‑1 (MQ=255)
                                                                                  gCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                           >  2:206659/1‑139 (MQ=255)
                                                                                   cGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgc                          <  1:206659/139‑1 (MQ=255)
                                                                                     aTTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCt                                                             >  2:1261200/1‑102 (MQ=255)
                                                                                     aTTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCt                                                             <  1:1261200/102‑1 (MQ=255)
                                                                                     aTTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGccgccg                        <  1:209252/139‑1 (MQ=255)
                                                                                      ttACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCgg                       >  2:6916/1‑139 (MQ=255)
                                                                                           gATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtttt                                                                                        <  2:637076/69‑1 (MQ=255)
                                                                                           gATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtttt                                                                                        >  1:637076/1‑69 (MQ=255)
                                                                                            aTTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaagt                 <  2:997128/139‑1 (MQ=255)
                                                                                              tttATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                                             <  2:277141/61‑1 (MQ=255)
                                                                                              tttATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                                             >  1:277141/1‑61 (MQ=255)
                                                                                                      cgcgcgGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgc                          <  2:1501709/120‑1 (MQ=255)
                                                                                                      cgcgcgGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgc                          >  1:1501709/1‑120 (MQ=255)
                                                                                                         cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGa                                                                                   >  2:119210/5‑61 (MQ=255)
                                                                                                         cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGa                                                                                   <  1:119210/57‑1 (MQ=255)
                                                                                                         cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                         >  2:299109/5‑71 (MQ=255)
                                                                                                         cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                         <  1:299109/67‑1 (MQ=255)
                                                                                                         cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                    <  1:722739/72‑1 (MQ=255)
                                                                                                         cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                    >  2:722739/5‑76 (MQ=255)
                                                                                                            gggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAt  <  1:6916/138‑1 (MQ=255)
                                                                                                                        |                                                                                                                              
ACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAT  >  NC_000913/3134703‑3134949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: