Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,140,294	T→C	S9G (AGC→GGC)	hybF ←	protein involved with the maturation of hydrogenases 1 and 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,140,294	0	T	C	100.0%	141.4 / NA	40	S9G (AGC→GGC)	hybF	protein involved with the maturation of hydrogenases 1 and 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

CCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCA  >  NC_000913/3140158‑3140416                                                                                                                                         |                                                                                                                           ccGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGccct                                                                                                                          <  2:642830/139‑1 (MQ=255)  cGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTg                                                                                                                         >  1:649314/1‑139 (MQ=255)  cGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTAAACGGCGCCCTg                                                                                                                         >  1:385075/1‑139 (MQ=255)         gACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAaga                                                                                                                  >  2:1054907/1‑139 (MQ=255)         gACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAaga                                                                                                                  >  2:1367979/1‑139 (MQ=255)                        aaaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAAc                                                                                                            >  2:323879/1‑130 (MQ=255)                        aaaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAAc                                                                                                            <  1:323879/130‑1 (MQ=255)                         aaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCAttt                                                                                                  >  2:1283269/1‑139 (MQ=255)                              gacggCGCTCTGCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCCTTTTCGcc                                                                                             <  1:980057/139‑1 (MQ=255)                                 ggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                >  1:1274626/1‑117 (MQ=255)                                 ggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                <  2:1274626/117‑1 (MQ=255)                                 ggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACGACTCATGCATTTTCGCCACt                                                                                          >  1:843704/1‑139 (MQ=255)                                        tcctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGa                                                                                   <  1:1100335/139‑1 (MQ=255)                                                    gagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCAt                                                                                                        <  1:219201/106‑1 (MQ=255)                                                    gagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCAt                                                                                                        >  2:219201/1‑106 (MQ=255)                                                         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                  >  1:533793/1‑139 (MQ=255)                                                         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                  <  1:1283269/139‑1 (MQ=255)                                                            gcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGa                                                                 <  1:274280/137‑1 (MQ=255)                                                            gcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGa                                                                 >  2:274280/1‑137 (MQ=255)                                                                aTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACGACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCt                                                           <  2:843704/139‑1 (MQ=255)                                                                aTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCt                                                           <  1:1367979/139‑1 (MQ=255)                                                                     cAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgtg                                                      <  2:649314/139‑1 (MQ=255)                                                                         cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGt                                                  <  1:821376/139‑1 (MQ=255)                                                                             cggcggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACAt                                              <  1:1054907/139‑1 (MQ=255)                                                                                          cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtt                                                                                                   <  1:811589/73‑1 (MQ=255)                                                                                          cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtt                                                                                                   >  2:811589/1‑73 (MQ=255)                                                                                                   gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                               <  1:1192704/52‑1 (MQ=255)                                                                                                   gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                               >  2:1192704/1‑52 (MQ=255)                                                                                                     gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                <  2:778053/49‑1 (MQ=255)                                                                                                     gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                >  1:778053/1‑49 (MQ=255)                                                                                                     gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGtgtg                                                    >  1:24475/1‑109 (MQ=255)                                                                                                     gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGtgtg                                                    <  2:24475/109‑1 (MQ=255)                                                                                                              ccTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTc             <  1:649846/139‑1 (MQ=255)                                                                                                              ccTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTc             >  1:790003/1‑139 (MQ=255)                                                                                                                 gCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtttt                                                                                                 <  2:138722/52‑1 (MQ=255)                                                                                                                 gCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtttt                                                                                                 >  1:138722/1‑52 (MQ=255)                                                                                                                         aTAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCa  <  2:533793/139‑1 (MQ=255)                                                                                                                             tttCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAg                     <  2:1367479/116‑1 (MQ=255)                                                                                                                             tttCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAg                     >  1:1367479/1‑116 (MQ=255)                                                                                                                                  aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTgg                              >  1:1397525/1‑102 (MQ=255)                                                                                                                                  aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTgg                              <  2:1397525/102‑1 (MQ=255)                                                                                                                                         |                                                                                                                           CCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCA  >  NC_000913/3140158‑3140416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑