Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 287,977 A→G M397V (ATG→GTG)  yagH → CP4‑6 prophage, putative xylosidase/arabinosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913287,9770AG100.0% 197.0 / NA 55M397V (ATG→GTG) yagHCP4‑6 prophage, putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/31);  total (24/31)

CGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAA  >  NC_000913/287853‑288093
                                                                                                                            |                                                                                                                    
cGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCg                                                                                                        <  2:1048294/139‑1 (MQ=255)
       ctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACt                                                                                                 >  1:373351/1‑139 (MQ=255)
       ctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACt                                                                                                 >  2:222562/1‑139 (MQ=255)
                  cccGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgc                                                                                      <  1:435221/139‑1 (MQ=255)
                  cccGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgc                                                                                      <  1:222562/139‑1 (MQ=255)
                           cttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTg                                                                             <  1:318463/139‑1 (MQ=255)
                            ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  2:720762/139‑1 (MQ=255)
                            ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  2:373351/139‑1 (MQ=255)
                             tACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                           <  2:1042631/139‑1 (MQ=255)
                                 gCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                                      >  1:1470072/1‑108 (MQ=255)
                                 gCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                                      <  2:1470072/108‑1 (MQ=255)
                                     tATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGc                                                                                                          <  1:1163909/100‑1 (MQ=255)
                                     tATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                                   >  1:610533/1‑139 (MQ=255)
                                     tATGGCAACGACTCGCTCAATTCGACCTTAACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGc                                                                                                          >  2:1163909/1‑100 (MQ=255)
                                     tATGGCAACGACTCGCTCAATTCGACCCTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                           <  2:332919/131‑1 (MQ=255)
                                     tATGGCAACGACTCGCTCAATTCGACCCTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                           >  1:332919/1‑131 (MQ=255)
                                               aCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTTCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaa                                                         <  2:953659/139‑1 (MQ=255)
                                               aCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaa                                                         >  2:738114/1‑139 (MQ=255)
                                                 tCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaaa                                                       >  1:1142885/1‑139 (MQ=255)
                                                    cTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                <  1:399825/111‑1 (MQ=255)
                                                    cTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                >  2:399825/1‑111 (MQ=255)
                                                     tCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTg                                                   >  1:454076/1‑139 (MQ=255)
                                                     tCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTg                                                   >  1:952512/1‑139 (MQ=255)
                                                      cAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTgg                                                  <  2:952512/139‑1 (MQ=255)
                                                      cAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTgg                                                  <  2:454076/139‑1 (MQ=255)
                                                        aTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAg                                                <  2:1142885/139‑1 (MQ=255)
                                                             aCCTTCACCCAATCGTCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            >  1:1410927/1‑106 (MQ=255)
                                                             aCCTTCACCCAATCGTCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  2:1410927/106‑1 (MQ=255)
                                                               cTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGc                                         <  1:43019/139‑1 (MQ=255)
                                                                        aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                                      >  1:1508196/1‑69 (MQ=255)
                                                                        aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                                      <  2:1508196/69‑1 (MQ=255)
                                                                        aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            >  1:170592/1‑95 (MQ=255)
                                                                        aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  2:170592/95‑1 (MQ=255)
                                                                        aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGc                                                           >  2:447513/1‑112 (MQ=255)
                                                                        aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGc                                                           <  1:447513/112‑1 (MQ=255)
                                                                              cGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACt                                           <  2:294505/122‑1 (MQ=255)
                                                                              cGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACt                                           >  1:294505/1‑122 (MQ=255)
                                                                               gTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  2:1165783/88‑1 (MQ=255)
                                                                               gTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            >  1:1165783/1‑88 (MQ=255)
                                                                                tGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            >  2:749074/1‑87 (MQ=255)
                                                                                tGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  1:749074/87‑1 (MQ=255)
                                                                                tGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctac                                                                    <  2:1081553/95‑1 (MQ=255)
                                                                                tGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctac                                                                    >  1:1081553/1‑95 (MQ=255)
                                                                                    gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa                    <  2:610533/139‑1 (MQ=255)
                                                                                    gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGAAGGGa                    <  1:1417369/139‑1 (MQ=255)
                                                                                       ccgcTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            >  2:687045/1‑80 (MQ=255)
                                                                                       ccgcTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  1:687045/80‑1 (MQ=255)
                                                                                          cTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGc                                                                                                    <  1:879153/53‑1 (MQ=255)
                                                                                          cTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGc                                                                                                    >  2:879153/1‑53 (MQ=255)
                                                                                                 caCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                                     <  2:143264/61‑1 (MQ=255)
                                                                                                 caCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                                     >  1:143264/1‑61 (MQ=255)
                                                                                                       gCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            >  2:105914/1‑64 (MQ=255)
                                                                                                       gCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                            <  1:105914/64‑1 (MQ=255)
                                                                                                         cTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCaaa  >  2:710937/1‑136 (MQ=255)
                                                                                                         cTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCaaa  <  1:710937/136‑1 (MQ=255)
                                                                                                                            |                                                                                                                    
CGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAA  >  NC_000913/287853‑288093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑