Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,162,308 | G→A | S121L (TCG→TTG) | ftsP ← | septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts) |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,162,308 | 0 | G | A | 100.0% | 127.1 / NA | 40 | S121L (TCG→TTG) | ftsP | septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts) |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/21); total (19/21) |
GCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGG > NC_000913/3162197‑3162433 | gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCa > 1:336640/1‑139 (MQ=255) ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc < 2:702219/139‑1 (MQ=255) ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCggg < 1:1057979/124‑1 (MQ=255) ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCggg > 2:1057979/1‑124 (MQ=255) ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc > 2:1297099/1‑139 (MQ=255) gCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg > 1:1384173/1‑120 (MQ=255) gCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg < 2:1384173/120‑1 (MQ=255) gCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAcc < 1:569937/139‑1 (MQ=255) tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCg < 2:558157/84‑1 (MQ=255) tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCg > 1:558157/1‑84 (MQ=255) tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAtt > 1:106828/1‑139 (MQ=255) tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAtt > 2:327638/1‑139 (MQ=255) tGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAtt < 2:106828/139‑1 (MQ=255) agagTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt < 2:384841/139‑1 (MQ=255) agagTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt < 2:1329797/139‑1 (MQ=255) agagTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt < 1:1037508/139‑1 (MQ=255) gTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCgg > 1:271136/1‑83 (MQ=255) gTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCgg < 2:271136/83‑1 (MQ=255) tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat > 2:646179/1‑58 (MQ=255) tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat < 1:646179/58‑1 (MQ=255) tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCg > 2:676850/1‑81 (MQ=255) tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCg < 1:676850/81‑1 (MQ=255) tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt > 1:554174/1‑139 (MQ=255) tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt > 2:254197/1‑139 (MQ=255) tGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTaa < 2:336640/139‑1 (MQ=255) cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc < 1:247769/132‑1 (MQ=255) cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc > 2:247769/1‑132 (MQ=255) cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt < 1:254197/139‑1 (MQ=255) aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGc < 2:554174/139‑1 (MQ=255) gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCggg > 2:128592/1‑53 (MQ=255) gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCggg < 1:128592/53‑1 (MQ=255) gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt > 2:22626/1‑109 (MQ=255) gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt < 1:22626/109‑1 (MQ=255) gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCa > 2:1283539/1‑139 (MQ=255) gTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCa > 2:357047/1‑139 (MQ=255) cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc < 2:32564/103‑1 (MQ=255) cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGc > 1:32564/1‑103 (MQ=255) gTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCgg < 1:327638/139‑1 (MQ=255) cAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGt < 1:861148/56‑1 (MQ=255) cAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGt > 2:861148/1‑56 (MQ=255) | GCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGG > NC_000913/3162197‑3162433 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |