Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,206	G→A	intergenic (‑241/‑1)	ygiF ← / → ygiM	inorganic triphosphatase/SH3 domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,206	0	G	A	100.0%	226.3 / NA	69	intergenic (‑241/‑1)	ygiF/ygiM	inorganic triphosphatase/SH3 domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/35);  total (34/35)

GGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTGATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATC  >  NC_000913/3201103‑3201339                                                                                                        |                                                                                                                                      ggCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  2:1447226/139‑1 (MQ=255)            acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  <  2:646725/98‑1 (MQ=255)            acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGcc                                                                                                                                  >  1:646725/1‑98 (MQ=255)            acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAgcg                                                                                         >  1:1037293/1‑139 (MQ=255)            acacAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAgcg                                                                                         >  2:328240/1‑139 (MQ=255)                  agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   <  2:114474/139‑1 (MQ=255)                  agGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   >  1:887322/1‑139 (MQ=255)                      ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGa                                                                                                                    >  2:409791/1‑102 (MQ=255)                      ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGa                                                                                                                    <  1:409791/102‑1 (MQ=255)                      ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  1:1301175/118‑1 (MQ=255)                      ttCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    >  2:1301175/1‑118 (MQ=255)                            ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               >  2:565459/1‑101 (MQ=255)                            ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGa                                                                                                               <  1:565459/101‑1 (MQ=255)                            ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:662844/139‑1 (MQ=255)                            ttGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:328240/139‑1 (MQ=255)                                 gTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   >  2:1495950/1‑92 (MQ=255)                                 gTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGAt                                                                                                                   <  1:1495950/92‑1 (MQ=255)                                      aTGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTgaagaa                                                               <  2:1037293/139‑1 (MQ=255)                                         cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTa                                                                                                      <  2:633612/97‑1 (MQ=255)                                         cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTa                                                                                                      >  1:633612/1‑97 (MQ=255)                                         cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  2:97846/1‑124 (MQ=255)                                         cTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  1:97846/124‑1 (MQ=255)                                          tttGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAAcgc                                                           >  1:453315/1‑139 (MQ=255)                                          tttGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAAcgc                                                           >  2:304290/1‑139 (MQ=255)                                              cGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         >  2:1344206/1‑121 (MQ=255)                                              cGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:1344206/121‑1 (MQ=255)                                                   cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAAc                                                                                                          <  1:177985/83‑1 (MQ=255)                                                   cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAAc                                                                                                          >  2:177985/1‑83 (MQ=255)                                                   cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGTCGTCTca                                                                           <  1:299761/114‑1 (MQ=255)                                                   cGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGTCGTCTca                                                                           >  2:299761/1‑114 (MQ=255)                                                        cTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcg                                               >  1:1047141/1‑137 (MQ=255)                                                        cTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCcg                                               <  2:1047141/137‑1 (MQ=255)                                                               cACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGa                                      <  2:453315/139‑1 (MQ=255)                                                                actactATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCt                                                        >  1:623005/1‑120 (MQ=255)                                                                actactATCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCt                                                        <  2:623005/120‑1 (MQ=255)                                                                   actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  1:1261694/98‑1 (MQ=255)                                                                   actaTCGTTCCACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  2:1261694/1‑98 (MQ=255)                                                                            ccACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCg                         >  2:606482/1‑139 (MQ=255)                                                                             cACTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGc                        <  2:810464/139‑1 (MQ=255)                                                                               cTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         <  1:1086269/88‑1 (MQ=255)                                                                               cTTTCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTcaca                                                                         >  2:1086269/1‑88 (MQ=255)                                                                                 ttCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  1:1251366/1‑84 (MQ=255)                                                                                 ttCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  2:1251366/84‑1 (MQ=255)                                                                                  tCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  1:1417132/58‑1 (MQ=255)                                                                                  tCCATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    >  2:1417132/1‑58 (MQ=255)                                                                                    cATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      <  2:635127/134‑1 (MQ=255)                                                                                    cATGACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      >  1:635127/1‑134 (MQ=255)                                                                                       gACAATAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCgg              <  2:887322/139‑1 (MQ=255)                                                                                          aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  1:12166/1‑75 (MQ=255)                                                                                          aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  2:12166/75‑1 (MQ=255)                                                                                          aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTc                           <  1:837791/123‑1 (MQ=255)                                                                                          aaTAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTc                           >  2:837791/1‑123 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    <  2:1387867/48‑1 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACt                                                                                                    >  1:1387867/1‑48 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   <  1:1248991/65‑1 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTg                                                                                   >  2:1248991/1‑65 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      >  2:665003/1‑126 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      <  1:665003/126‑1 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGAt         >  1:1438123/1‑139 (MQ=255)                                                                                            tAACGACAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGAt         >  1:1005755/1‑139 (MQ=255)                                                                                                 aCAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCa                                                                                                <  1:1257628/47‑1 (MQ=255)                                                                                                 aCAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCa                                                                                                >  2:1257628/1‑47 (MQ=255)                                                                                                  cAGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTAt   <  1:304290/139‑1 (MQ=255)                                                                                                   aGCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATc  >  2:910066/1‑139 (MQ=255)                                                                                                    gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           >  2:155407/1‑65 (MQ=255)                                                                                                    gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTca                                                                           <  1:155407/65‑1 (MQ=255)                                                                                                    gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      >  2:520817/1‑118 (MQ=255)                                                                                                    gCCTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAg                      <  1:520817/118‑1 (MQ=255)                                                                                                     ccTAATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACt                                                                                    <  2:128253/55‑1 (MQ=255)                                                                                                        |                                                                                                                                      GGCAAGACCTGACACAGAGGATTCCGATTGATGTCGAATGCTTTGCGTAGCGACACTGATGCCACTACTATCGTTCCACTTTCCATGACAATAACGACAGCCTGATGCCAAAATTACGCCTGATCGGATTAACTTTACTCGCACTTAGCGCGACTGCCGTCTCACACGCTGAAGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAGATCATTATC  >  NC_000913/3201103‑3201339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑