Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,232,174 C→T N170N (AAC→AAT)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,232,1740CT100.0% 134.2 / NA 42N170N (AAC→AAT) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/21);  total (21/21)

CGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTT  >  NC_000913/3232045‑3232293
                                                                                                                                 |                                                                                                                       
cGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTg                                                                                                                >  1:635881/1‑139 (MQ=255)
     cccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCt                                                                                                           <  2:1173734/139‑1 (MQ=255)
                                    aCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggc                                                                            >  2:3982/1‑139 (MQ=255)
                                        aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGCTCAATGAATTTCTGa                                                                                                               <  2:1007874/100‑1 (MQ=255)
                                        aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGa                                                                                                               >  1:1007874/1‑100 (MQ=255)
                                        aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAg                                                                                  <  2:615289/129‑1 (MQ=255)
                                        aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAg                                                                                  >  1:615289/1‑129 (MQ=255)
                                        aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGgcggcg                                                                        >  2:401607/1‑139 (MQ=255)
                                        aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGgcggcg                                                                        >  1:658851/1‑139 (MQ=255)
                                         tCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                       <  1:3982/139‑1 (MQ=255)
                                                     cccGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCg                                                           >  2:626299/1‑139 (MQ=255)
                                                         cTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCa                                                                                                      <  2:336031/92‑1 (MQ=255)
                                                         cTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCa                                                                                                      >  1:336031/1‑92 (MQ=255)
                                                            cgcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGa                                                    <  1:626299/139‑1 (MQ=255)
                                                                   cTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                             <  2:436312/139‑1 (MQ=255)
                                                                    tGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGt                                            >  2:1014865/1‑139 (MQ=255)
                                                                       cgcgGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGAGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGa                                         >  2:916515/1‑139 (MQ=255)
                                                                           ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCg                                                                                            <  1:842533/84‑1 (MQ=255)
                                                                           ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCg                                                                                            >  2:842533/1‑84 (MQ=255)
                                                                           ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagta                                     >  1:595369/1‑139 (MQ=255)
                                                                              ggCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtg                                  <  1:1014865/139‑1 (MQ=255)
                                                                                  ggATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                              <  1:401607/139‑1 (MQ=255)
                                                                                        gacgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                                   >  2:1130156/1‑112 (MQ=255)
                                                                                        gacgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                                   <  1:1130156/112‑1 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGATTTTCTGACGCTGCGCACCAATCAGCGTa                                                                                          >  2:870636/1‑58 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCAcc                                                                                                    >  2:1183512/1‑48 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCAcc                                                                                                    <  1:1183512/48‑1 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAAt                                                                                                 >  2:105527/1‑51 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAAt                                                                                                 <  1:105527/51‑1 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                          <  1:870636/58‑1 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTAc                                                                   >  2:119594/1‑81 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTAc                                                                   <  1:119594/81‑1 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                              <  1:1303214/118‑1 (MQ=255)
                                                                                                       aTGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                              >  2:1303214/1‑118 (MQ=255)
                                                                                                         gggTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                  <  1:370491/96‑1 (MQ=255)
                                                                                                         gggTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                  >  2:370491/1‑96 (MQ=255)
                                                                                                         gggTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAAc       <  2:635881/139‑1 (MQ=255)
                                                                                                              ccGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGc                                                                <  1:1219265/77‑1 (MQ=255)
                                                                                                              ccGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGc                                                                >  2:1219265/1‑77 (MQ=255)
                                                                                                              ccGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGAGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACtt  <  1:916515/139‑1 (MQ=255)
                                                                                                               cGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAAc                                                             <  1:1284055/79‑1 (MQ=255)
                                                                                                               cGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAAc                                                             >  2:1284055/1‑79 (MQ=255)
                                                                                                                                 |                                                                                                                       
CGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTT  >  NC_000913/3232045‑3232293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑