Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,255,020 G→A *234* (TAG→TAA yhaK → redox‑sensitive bicupin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,255,0200GA100.0% 95.5 / NA 30*234* (TAG→TAAyhaKredox‑sensitive bicupin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/16);  total (14/16)

TCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAT  >  NC_000913/3254888‑3255129
                                                                                                                                    |                                                                                                             
tCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGttt                                                                                                         <  1:30802/139‑1 (MQ=255)
   tGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTa                                                                                                      <  2:1352224/139‑1 (MQ=255)
        ttACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGTTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                 >  2:151280/1‑139 (MQ=255)
        ttACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                 <  1:202265/139‑1 (MQ=255)
                  tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTa                                                                                                      >  1:1507048/1‑124 (MQ=255)
                  tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTa                                                                                                      <  2:1507048/124‑1 (MQ=255)
                  tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                       <  1:1062471/139‑1 (MQ=255)
                                          cGGTGATGGGGCGTTTATTCGTGACGAGGTTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcg                                                               <  1:151280/139‑1 (MQ=255)
                                          cGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTATCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcg                                                               <  2:952748/139‑1 (MQ=255)
                                               aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                       <  2:562756/110‑1 (MQ=255)
                                               aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                       >  1:562756/1‑110 (MQ=255)
                                               aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGc                                                          >  2:1035225/1‑139 (MQ=255)
                                                 ggggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCg                                                        >  2:1114051/1‑139 (MQ=255)
                                                    gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAgg                                                                                                  <  1:490648/94‑1 (MQ=255)
                                                    gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAgg                                                                                                  >  2:490648/1‑94 (MQ=255)
                                                       tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGttttt                                                                                                       >  1:855595/1‑86 (MQ=255)
                                                       tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGttttt                                                                                                       <  2:855595/86‑1 (MQ=255)
                                                         tATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGcc                                                >  2:560463/1‑139 (MQ=255)
                                                                 aCGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGtggtgg                                        <  1:1114051/139‑1 (MQ=255)
                                                                                cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                       <  2:417942/77‑1 (MQ=255)
                                                                                cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                       >  1:417942/1‑77 (MQ=255)
                                                                                                      gcgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                 >  1:563497/1‑45 (MQ=255)
                                                                                                      gcgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                 <  2:563497/45‑1 (MQ=255)
                                                                                                      gcgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGc                                                          >  2:450717/1‑84 (MQ=255)
                                                                                                      gcgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGc                                                          <  1:450717/84‑1 (MQ=255)
                                                                                                       cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                   >  2:549074/1‑42 (MQ=255)
                                                                                                       cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                   <  1:549074/42‑1 (MQ=255)
                                                                                                       cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt  >  1:575049/1‑139 (MQ=255)
                                                                                                         cgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                       <  2:204012/52‑1 (MQ=255)
                                                                                                         cgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                       >  1:204012/1‑52 (MQ=255)
                                                                                                                                    |                                                                                                             
TCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAT  >  NC_000913/3254888‑3255129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: