Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,012	A→G	S200G (AGT→GGT)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,012	0	A	G	100.0%	219.6 / NA	61	S200G (AGT→GGT)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/29);  total (32/29)

TCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTA  >  NC_000913/3268877‑3269147                                                                                                                                        |                                                                                                                                        tctctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGtt                                                                                                                                      <  1:1455338/139‑1 (MQ=255)   tctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCa                                                                                                                                    >  1:1234327/1‑139 (MQ=255)        aCTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAAcaca                                                                                                                               >  2:1182199/1‑139 (MQ=255)             cGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                          >  1:169308/1‑139 (MQ=255)                 aTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcac                                                                                                                      >  1:1088436/1‑139 (MQ=255)                  tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     <  1:1182199/139‑1 (MQ=255)                       aTTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGtt                                                                                                                                      <  2:235904/117‑1 (MQ=255)                       aTTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGtt                                                                                                                                      >  1:235904/1‑117 (MQ=255)                       aTTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     >  1:1493121/1‑134 (MQ=255)                       aTTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     <  2:1493121/134‑1 (MQ=255)                                      aTTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     <  1:701070/119‑1 (MQ=255)                                      aTTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     >  2:701070/1‑119 (MQ=255)                                         tCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAtctc                                                                                                                >  2:1153514/1‑121 (MQ=255)                                         tCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAtctc                                                                                                                <  1:1153514/121‑1 (MQ=255)                                                       atGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                          <  2:1094362/97‑1 (MQ=255)                                                       atGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                          >  1:1094362/1‑97 (MQ=255)                                                       atGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACaa                                                                                                                    >  2:1259204/1‑103 (MQ=255)                                                       atGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACaa                                                                                                                    <  1:1259204/103‑1 (MQ=255)                                                         ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     >  2:1211349/1‑100 (MQ=255)                                                         ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     <  2:353009/100‑1 (MQ=255)                                                         ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     <  1:1211349/100‑1 (MQ=255)                                                         ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     >  1:353009/1‑100 (MQ=255)                                                            tAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaata                                                                           <  1:1163552/139‑1 (MQ=255)                                                              aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAt                                                                                                                   >  1:505829/1‑97 (MQ=255)                                                              aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAt                                                                                                                   <  2:505829/97‑1 (MQ=255)                                                              aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                               >  1:602044/1‑101 (MQ=255)                                                              aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                               <  2:602044/101‑1 (MQ=255)                                                               tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAAcaca                                                                                                                               >  1:1303866/1‑84 (MQ=255)                                                               tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAAcaca                                                                                                                               <  2:1303866/84‑1 (MQ=255)                                                                    ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCt                                                                                                              <  1:1293737/96‑1 (MQ=255)                                                                    ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCt                                                                                                              >  2:1293737/1‑96 (MQ=255)                                                                    ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                                  >  2:467103/1‑124 (MQ=255)                                                                    ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                                  <  1:467103/124‑1 (MQ=255)                                                                         aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGata                                                              >  2:326848/1‑139 (MQ=255)                                                                              ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     >  2:501013/1‑79 (MQ=255)                                                                              ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                     <  1:501013/79‑1 (MQ=255)                                                                              ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGa                                                                                                          <  2:1014756/90‑1 (MQ=255)                                                                              ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGa                                                                                                          >  1:1014756/1‑90 (MQ=255)                                                                                   aaTTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTga                                                    <  2:1088436/139‑1 (MQ=255)                                                                                        aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                          <  1:651101/64‑1 (MQ=255)                                                                                        aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                          >  2:651101/1‑64 (MQ=255)                                                                                        aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGaaa                                               >  2:160054/1‑139 (MQ=255)                                                                                               caaATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                      >  2:230182/1‑93 (MQ=255)                                                                                               caaATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTGGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                      <  1:230182/93‑1 (MQ=255)                                                                                                 aaTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGt                                      <  1:160054/139‑1 (MQ=255)                                                                                                  aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTaaa                                                                                              >  1:472246/1‑82 (MQ=255)                                                                                                  aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTaaa                                                                                              <  2:472246/82‑1 (MQ=255)                                                                                                            tGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgt                           >  2:1038084/1‑139 (MQ=255)                                                                                                             gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                            >  2:746914/1‑89 (MQ=255)                                                                                                             gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                            <  1:746914/89‑1 (MQ=255)                                                                                                               cAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgttgt                        <  2:1234327/139‑1 (MQ=255)                                                                                                                    tGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                   <  2:169308/139‑1 (MQ=255)                                                                                                                       ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                   <  2:999618/136‑1 (MQ=255)                                                                                                                       ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                   >  1:999618/1‑136 (MQ=255)                                                                                                                       ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGa                >  2:222335/1‑139 (MQ=255)                                                                                                                            gTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTg           >  1:465912/1‑139 (MQ=255)                                                                                                                               aaTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGa                                                                                                          >  1:20000/1‑41 (MQ=255)                                                                                                                               aaTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGa                                                                                                          <  2:20000/41‑1 (MQ=255)                                                                                                                               aaTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGgag        >  2:597346/1‑139 (MQ=255)                                                                                                                                 ttttGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGAc      >  2:888863/1‑139 (MQ=255)                                                                                                                                     gTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTa  <  1:597346/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTA  >  NC_000913/3268877‑3269147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑