Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,277,867 C→T V267I (GTC→ATC)  agaR ← transcriptional repressor of the aga regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,8670CT100.0% 253.2 / NA 77V267I (GTC→ATC) agaRtranscriptional repressor of the aga regulon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/38);  total (39/38)

AAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAA  >  NC_000913/3277739‑3278000
                                                                                                                                |                                                                                                                                     
aaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACt                                                                                                                             >  1:54769/1‑139 (MQ=255)
               cctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCGTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCttt                                                                                                              >  2:336270/1‑139 (MQ=255)
                ctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTc                                                                                                             <  1:1236327/139‑1 (MQ=255)
                  gCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGc                                                                                                           >  2:28544/1‑139 (MQ=255)
                      aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATc                                                                                                                                >  2:1051478/1‑114 (MQ=255)
                      aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATc                                                                                                                                <  1:1051478/114‑1 (MQ=255)
                      aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc                                                                                                       >  2:776600/1‑139 (MQ=255)
                      aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc                                                                                                       >  2:1425611/1‑139 (MQ=255)
                      aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc                                                                                                       >  1:1218338/1‑139 (MQ=255)
                        tGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGt                                                                                                        >  1:118057/1‑136 (MQ=255)
                        tGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGt                                                                                                        <  2:118057/136‑1 (MQ=255)
                         gggAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCGTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCtt                                                                                                    <  1:336270/139‑1 (MQ=255)
                          ggAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         <  1:666044/133‑1 (MQ=255)
                          ggAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         >  2:666044/1‑133 (MQ=255)
                          ggAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTc                                                                                                   >  2:145944/1‑139 (MQ=255)
                           gAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTcc                                                                                                  <  2:54769/139‑1 (MQ=255)
                                         agaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                    >  1:1369371/1‑139 (MQ=255)
                                         agaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                    >  1:449189/1‑139 (MQ=255)
                                          gaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGa                                                                                   <  2:1332256/139‑1 (MQ=255)
                                           aaaCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGaa                                                                                  <  1:1425611/139‑1 (MQ=255)
                                              cAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGAGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGc                                                                               >  1:1258307/1‑139 (MQ=255)
                                                        ccATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaac                                                                     <  2:1369371/139‑1 (MQ=255)
                                                         cATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    <  1:78005/139‑1 (MQ=255)
                                                         cATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    <  1:1396778/139‑1 (MQ=255)
                                                           ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGcc                                                                                                                 >  1:422253/1‑92 (MQ=255)
                                                           ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGcc                                                                                                                 <  2:422253/92‑1 (MQ=255)
                                                           ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         <  1:1491288/100‑1 (MQ=255)
                                                           ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         >  2:1491288/1‑100 (MQ=255)
                                                           ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    <  2:1347716/137‑1 (MQ=255)
                                                           ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    >  1:1347716/1‑137 (MQ=255)
                                                           ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAAt                                                                  >  2:251433/1‑139 (MQ=255)
                                                              atagtgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                               <  1:251433/136‑1 (MQ=255)
                                                                 gtgtTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCAt                                                            <  2:1218338/139‑1 (MQ=255)
                                                                    ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         >  2:1241685/1‑123 (MQ=255)
                                                                    ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         <  1:1241685/123‑1 (MQ=255)
                                                                    ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTc                                                         <  2:804786/139‑1 (MQ=255)
                                                                    ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTc                                                         >  1:697711/1‑139 (MQ=255)
                                                                     tCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCg                                                        <  1:776600/139‑1 (MQ=255)
                                                                      cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCa                                                                                                          <  2:1062825/88‑1 (MQ=255)
                                                                      cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCa                                                                                                          >  1:1062825/1‑88 (MQ=255)
                                                                          aTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                   <  1:1183248/139‑1 (MQ=255)
                                                                           tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGTAATGCCTTCATCAACAATGa                                                                <  2:888051/125‑1 (MQ=255)
                                                                           tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCGACAATGa                                                                >  1:888051/1‑125 (MQ=255)
                                                                            gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                                 <  2:848704/139‑1 (MQ=255)
                                                                              ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAAt                                                                                                                                 <  2:1158796/57‑1 (MQ=255)
                                                                              ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAAt                                                                                                                                 >  1:1158796/1‑57 (MQ=255)
                                                                              ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         <  2:591578/81‑1 (MQ=255)
                                                                              ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         >  1:591578/1‑81 (MQ=255)
                                                                              ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTg                                                >  1:1506706/1‑138 (MQ=255)
                                                                              ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTg                                                <  2:1506706/138‑1 (MQ=255)
                                                                                gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAAt                                                                                                                                 <  1:1414464/55‑1 (MQ=255)
                                                                                gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAAt                                                                                                                                 >  2:1414464/1‑55 (MQ=255)
                                                                                gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAAAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                    <  2:399873/100‑1 (MQ=255)
                                                                                gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAAAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                    >  1:399873/1‑100 (MQ=255)
                                                                                 ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCGACCCCAGCCTTTCGCAg                                                                                                         >  1:1420866/1‑78 (MQ=255)
                                                                                 ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCGACCCCAGCCTTTCGCAg                                                                                                         <  2:1420866/78‑1 (MQ=255)
                                                                                 ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGc                                                                                                                  <  2:131034/69‑1 (MQ=255)
                                                                                 ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGc                                                                                                                  >  1:131034/1‑69 (MQ=255)
                                                                                     aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                               >  2:339084/1‑84 (MQ=255)
                                                                                     aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                               <  1:339084/84‑1 (MQ=255)
                                                                                     aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                        <  1:145944/139‑1 (MQ=255)
                                                                                               gtTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATg                              <  2:449189/139‑1 (MQ=255)
                                                                                                       gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTgg                      >  1:1485204/1‑139 (MQ=255)
                                                                                                       gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTgg                      >  2:1233802/1‑139 (MQ=255)
                                                                                                              tCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGAtt               <  2:1258307/139‑1 (MQ=255)
                                                                                                                  ttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt                                                                                             >  1:600284/1‑57 (MQ=255)
                                                                                                                  ttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt                                                                                             <  2:600284/57‑1 (MQ=255)
                                                                                                                         tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                      >  1:510849/1‑89 (MQ=255)
                                                                                                                         tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                      <  2:510849/89‑1 (MQ=255)
                                                                                                                         tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCACAGGAATGCCTTCATCAACAATGATCATGTCGa                                                       >  2:1477174/1‑88 (MQ=255)
                                                                                                                         tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCACAGGAATGCCTTCATCAACAATGATCATGTCGa                                                       <  1:1477174/88‑1 (MQ=255)
                                                                                                                          ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGa   >  1:207832/1‑139 (MQ=255)
                                                                                                                          ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGa   >  2:576876/1‑139 (MQ=255)
                                                                                                                           cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc                                                                           <  2:468453/66‑1 (MQ=255)
                                                                                                                           cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc                                                                           >  1:468453/1‑66 (MQ=255)
                                                                                                                           cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGaa  >  1:454074/1‑139 (MQ=255)
                                                                                                                             cGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCt                                                                                                     >  1:903879/1‑38 (MQ=255)
                                                                                                                                |                                                                                                                                     
AAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAA  >  NC_000913/3277739‑3278000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑