| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,285,690 | C→T | V71V (GTC→GTT) | agaD → | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,285,690 | 0 | C | T | 100.0% | 168.7 / NA | 54 | V71V (GTC→GTT) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/25); total (29/25) | |||||||||||
CGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACT > NC_000913/3285555‑3285816 | cGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa > 2:1322465/1‑139 (MQ=255)cGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa > 1:1470546/1‑139 (MQ=255) gAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa > 2:1200162/1‑138 (MQ=255) gAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa < 1:1200162/138‑1 (MQ=255) gAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGAt < 1:373258/139‑1 (MQ=255) ggcgggcgGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATgggg > 1:1493671/1‑139 (MQ=255) aCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa < 1:1322465/139‑1 (MQ=255) cTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATg < 2:1493671/139‑1 (MQ=255) ggCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGAt > 2:302637/1‑117 (MQ=255) ggCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGAt < 1:302637/117‑1 (MQ=255) gCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa < 1:414259/133‑1 (MQ=255) gCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa > 2:414259/1‑133 (MQ=255) tGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGaaaa > 2:1070365/1‑139 (MQ=255) ccGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATg > 1:18466/1‑108 (MQ=255) ccGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATg < 2:18466/108‑1 (MQ=255) cGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATg > 2:845069/1‑125 (MQ=255) cGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATg < 1:845069/125‑1 (MQ=255) tCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGaaaa > 1:1167486/1‑139 (MQ=255) ccTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATggg < 1:1233888/105‑1 (MQ=255) ccTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATggg > 2:1233888/1‑105 (MQ=255) tGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACcg < 2:1048264/139‑1 (MQ=255) tGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACcg > 1:1048264/1‑139 (MQ=255) aGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGacac < 2:1167486/139‑1 (MQ=255) gacgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGgaaga < 2:116756/107‑1 (MQ=255) gacgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGgaaga > 1:116756/1‑107 (MQ=255) acgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa < 2:458104/99‑1 (MQ=255) acgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa > 1:458104/1‑99 (MQ=255) gggCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa < 1:1070365/139‑1 (MQ=255) aGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTg > 2:263686/1‑139 (MQ=255) cggcgATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTAGTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAGGGCCTCAAAGTGACACTGTTTGGccg > 2:331415/1‑138 (MQ=255) gATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGa < 1:331415/139‑1 (MQ=255) aCCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa > 2:163400/1‑113 (MQ=255) aCCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa < 1:163400/113‑1 (MQ=255) aCCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTgg < 2:1470546/139‑1 (MQ=255) gAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGgcgatg < 1:263686/139‑1 (MQ=255) attaATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGAttt > 1:1266915/1‑139 (MQ=255) tACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAAc > 2:7323/1‑67 (MQ=255) tACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAAc < 1:7323/67‑1 (MQ=255) acccacccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGac < 1:268782/72‑1 (MQ=255) acccacccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGac > 2:268782/1‑72 (MQ=255) gcccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGtt > 1:225049/2‑136 (MQ=255) gcccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGtt < 2:225049/135‑1 (MQ=255) acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc < 2:165674/109‑1 (MQ=255) acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc > 1:165674/1‑109 (MQ=255) acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGAttt > 2:1393854/1‑129 (MQ=255) acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGAttt < 1:1393854/129‑1 (MQ=255) acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTAc > 1:657282/1‑139 (MQ=255) acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTAc > 2:452345/1‑139 (MQ=255) cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc < 2:469331/108‑1 (MQ=255) cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc > 1:469331/1‑108 (MQ=255) cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACt > 2:621697/1‑139 (MQ=255) cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTCGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACt > 2:903156/1‑139 (MQ=255) ccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa > 2:306135/1‑90 (MQ=255) ccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa < 1:306135/90‑1 (MQ=255) | CGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACT > NC_000913/3285555‑3285816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |