Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,438	A→G	T155T (ACA→ACG)	yhbO →	stress‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,438	0	A	G	100.0%	195.6 / NA	54	T155T (ACA→ACG)	yhbO	stress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

CAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACA  >  NC_000913/3299306‑3299572                                                                                                                                     |                                                                                                                                       cAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAgccggacgccggac                                                                                                                                  >  2:1320408/1‑139 (MQ=255)    cgcCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGAt                                                                                                                               >  2:19484/1‑139 (MQ=255)          tgtgATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                         <  1:395494/139‑1 (MQ=255)                      gcgcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACcgc                                                                                                             >  1:1312654/1‑139 (MQ=255)                       cgcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACcgcg                                                                                                            <  2:1312654/139‑1 (MQ=255)                        gcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                           >  1:1056064/1‑139 (MQ=255)                        gcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                           >  2:184626/1‑139 (MQ=255)                                aCCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTAc                                                                                                   >  1:848004/1‑139 (MQ=255)                                        tAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                           <  1:1320408/139‑1 (MQ=255)                                           aCCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGt                                                                                        <  2:609680/139‑1 (MQ=255)                                                     attGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCt                                                                                            <  1:861609/125‑1 (MQ=255)                                                     attGATGTTAAAAATGCAGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCt                                                                                            >  2:861609/1‑125 (MQ=255)                                                           gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                           <  2:834045/104‑1 (MQ=255)                                                           gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                           >  1:834045/1‑104 (MQ=255)                                                           gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc                                                                        >  2:810241/1‑139 (MQ=255)                                                              aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGAt                                                                                                                               >  1:491101/1‑81 (MQ=255)                                                              aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGAt                                                                                                                               <  2:491101/81‑1 (MQ=255)                                                              aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCaa                                                                     >  1:1469729/1‑139 (MQ=255)                                                              aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCaa                                                                     >  1:702292/1‑139 (MQ=255)                                                                       ggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAttttt                                                            <  2:1056064/139‑1 (MQ=255)                                                                         cgcgGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAttttttt                                                          >  2:1218143/1‑139 (MQ=255)                                                                            ggAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTAcc                                                       <  1:19484/139‑1 (MQ=255)                                                                                       aTCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCg                                            <  2:702292/139‑1 (MQ=255)                                                                                        tCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGttt                                                                                                                   >  2:88058/1‑67 (MQ=255)                                                                                        tCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGttt                                                                                                                   <  1:88058/67‑1 (MQ=255)                                                                                            gAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGtgttg                                       <  1:11667/139‑1 (MQ=255)                                                                                             tataagagtcagATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGtgttgt                                      >  1:1089320/12‑139 (MQ=255)                                                                                                  gTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTg                                 >  1:1363900/1‑139 (MQ=255)                                                                                                  gTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTg                                 >  2:1361308/1‑139 (MQ=255)                                                                                                  gTGGTTAATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                                   >  1:563298/1‑41 (MQ=255)                                                                                                  gTGGTTAATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                                   <  2:563298/41‑1 (MQ=255)                                                                                                    ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                                     >  2:691011/1‑37 (MQ=38)                                                                                                    ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                                     <  1:691011/37‑1 (MQ=38)                                                                                                    ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                                      >  1:1190472/1‑68 (MQ=255)                                                                                                    ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                                      <  2:1190472/68‑1 (MQ=255)                                                                                                              gATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCa                     >  2:28755/1‑139 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                           >  1:1034127/1‑64 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                           <  2:1034127/64‑1 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCt                                                                                    <  1:529691/71‑1 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCt                                                                                    >  2:529691/1‑71 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTcgcg                                                                            <  1:543304/79‑1 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTcgcg                                                                            >  2:543304/1‑79 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTAcc                                                       >  1:23811/1‑100 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTAcc                                                       <  2:23811/100‑1 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAg                <  2:848004/139‑1 (MQ=255)                                                                                                                   gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAg                <  1:184626/139‑1 (MQ=255)                                                                                                                    cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGc                                                                                      >  2:312220/1‑68 (MQ=255)                                                                                                                    cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGc                                                                                      <  1:312220/68‑1 (MQ=255)                                                                                                                          aCCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGAtt         >  1:357174/1‑139 (MQ=255)                                                                                                                            cAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAtttt                                                             >  2:188556/1‑85 (MQ=255)                                                                                                                            cAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAtttt                                                             <  1:188556/85‑1 (MQ=255)                                                                                                                            cAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTc       >  2:1423717/1‑139 (MQ=255)                                                                                                                              gccggacgccggacGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGGCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGcc                                                 <  2:1046350/95‑1 (MQ=255)                                                                                                                              gccggacgccggacGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGcc                                                 >  1:1046350/1‑95 (MQ=255)                                                                                                                                 ggACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGc                                                  <  2:151890/91‑1 (MQ=255)                                                                                                                                 ggACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGc                                                  >  1:151890/1‑91 (MQ=255)                                                                                                                                 ggACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCcaca  >  2:911878/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       CAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACA  >  NC_000913/3299306‑3299572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑