Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,455	G→A	*336* (TAG→TAA)	yhbW →	putative luciferase‑like monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,455	0	G	A	97.3%	107.4 / NA	37	*336* (TAG→TAA)	yhbW	putative luciferase‑like monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (17/19);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCATGGCTTGCAGTCGATCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACC  >  NC_000913/3304321‑3304585                                                                                                                                       |                                                                                                                                   cGTCATGGCTTGCAGTCGATCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAatgtg                                                                                                                                >  2:731788/1‑139 (MQ=255)   tCATGGCTTGCAGTCGATCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTc                                                                                                                              >  2:494040/1‑139 (MQ=255)                   aTCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCt                                                                                                              >  1:503995/1‑139 (MQ=255)                          gcgAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGa                                                                                                       >  2:478242/1‑139 (MQ=255)                              aaCCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTg                                                                                                   >  2:289983/1‑139 (MQ=255)                                    cGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  2:935110/139‑1 (MQ=255)                                    cGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  2:169613/139‑1 (MQ=255)                                    cGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  1:494040/139‑1 (MQ=255)                                    cGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  1:478242/139‑1 (MQ=255)                                    cGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  2:503995/139‑1 (MQ=255)                                                       aaCGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  2:717270/120‑1 (MQ=255)                                                       aaCGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             >  1:717270/1‑120 (MQ=255)                                                                     cGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCAt                                                            <  1:731788/139‑1 (MQ=255)                                                                           ccaGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCa                                                      <  1:289983/139‑1 (MQ=255)                                                                                     cTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACa                                                                                   <  1:1387189/100‑1 (MQ=255)                                                                                     cTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACa                                                                                   >  2:1387189/1‑100 (MQ=255)                                                                                                     tGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGAt                            >  2:321224/1‑139 (MQ=255)                                                                                                       gCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCAt                                                     <  2:1047292/112‑1 (MQ=255)                                                                                                       gCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCAt                                                     >  1:1047292/1‑112 (MQ=255)                                                                                                       gCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATaa                          >  2:829283/1‑139 (MQ=255)                                                                                                        cGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGAt                                                                         <  1:380987/91‑1 (MQ=255)                                                                                                        cGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGAt                                                                         >  2:380987/1‑91 (MQ=255)                                                                                                         gatggatgTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCAGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAgg                        <  1:321224/139‑1 (MQ=255)                                                                                                                 ttAAGGAAGAGTTGTT‑GGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  2:1095863/61‑1 (MQ=255)                                                                                                                 ttAAGGAAGAGTTGTT‑GGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             >  1:1095863/1‑61 (MQ=255)                                                                                                                         gagTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTgg                                                                                                  <  2:1306731/49‑1 (MQ=255)                                                                                                                         gagTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTgg                                                                                                  >  1:1306731/1‑49 (MQ=255)                                                                                                                         gagTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGAc                                                                              >  2:1262744/1‑69 (MQ=255)                                                                                                                         gagTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGAc                                                                              <  1:1262744/69‑1 (MQ=255)                                                                                                                           gttgttGGGATAATGTGTCTTAACGCGGGTAGCCTTATCCGAGCTGGCaa                                                                                               <  2:887392/50‑1 (MQ=255)                                                                                                                           gttgttGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCaa                                                                                               >  1:887392/1‑50 (MQ=255)                                                                                                                           gttgttGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTa                                                                                     >  2:399634/1‑60 (MQ=255)                                                                                                                           gttgttGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTa                                                                                     <  1:399634/60‑1 (MQ=255)                                                                                                                               ttgGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACAcc  >  1:182953/1‑139 (MQ=255)                                                                                                                                 gggATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             <  1:540214/46‑1 (MQ=255)                                                                                                                                 gggATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                             >  2:540214/1‑46 (MQ=255)                                                                                                                                   gATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAgg                                                        >  2:1377896/1‑81 (MQ=255)                                                                                                                                   gATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAgg                                                        <  1:1377896/81‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   CGTCATGGCTTGCAGTCGATCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACC  >  NC_000913/3304321‑3304585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑