Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,309,007 G→A intergenic (‑83/+26) nlpI ← / ← pnp lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,0070GA100.0% 135.5 / NA 43intergenic (‑83/+26)nlpI/pnplipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/20);  total (23/20)

TGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTCCAGAA  >  NC_000913/3308889‑3309142
                                                                                                                      |                                                                                                                                       
tGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGc                                                                                                                    >  2:1272586/1‑139 (MQ=255)
                  gcgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGtt                                                                                                  >  1:806983/1‑139 (MQ=255)
                   cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTc                                                                                                 >  2:813238/1‑139 (MQ=255)
                    gcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTca                                                                                                >  2:1071216/1‑139 (MQ=255)
                           aGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                                         <  1:1071216/139‑1 (MQ=255)
                                     tCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCgg                                                                               <  2:806983/139‑1 (MQ=255)
                                       ccACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtg                                                                             >  1:1423044/1‑139 (MQ=255)
                                           tCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                                       >  2:1160271/1‑109 (MQ=255)
                                           tCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                                       <  1:1160271/109‑1 (MQ=255)
                                             ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                                       <  2:1423044/139‑1 (MQ=255)
                                                       cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                            <  2:279370/92‑1 (MQ=255)
                                                       cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                            >  1:279370/1‑92 (MQ=255)
                                                       cGGTTGAATGAACGTCCTGATCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                             >  1:1006231/1‑139 (MQ=255)
                                                             aatgaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                                       <  1:1442914/91‑1 (MQ=255)
                                                             aatgaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                                       >  2:1442914/1‑91 (MQ=255)
                                                                      ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgct                                                                           <  1:1477204/110‑1 (MQ=255)
                                                                      ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgct                                                                           >  2:1477204/1‑110 (MQ=255)
                                                                          ttCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGATCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTa                                          <  2:1069117/139‑1 (MQ=255)
                                                                               ggTTGCTTACAAGGCGTCCTGCCCAGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt                                     >  1:920701/1‑139 (MQ=255)
                                                                               ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                         <  1:1297578/71‑1 (MQ=255)
                                                                               ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                         >  2:1297578/1‑71 (MQ=255)
                                                                               ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt                                     >  2:585607/1‑139 (MQ=255)
                                                                                gTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGcc                                                                                          <  1:636768/85‑1 (MQ=255)
                                                                                gTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGcc                                                                                          >  2:636768/1‑85 (MQ=255)
                                                                                 ttGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCa                                   <  2:920701/139‑1 (MQ=255)
                                                                                    cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCgg                                                                                                                                >  2:27442/1‑43 (MQ=255)
                                                                                    cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCgg                                                                                                                                <  1:27442/43‑1 (MQ=255)
                                                                                    cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGa                                                                                                                               >  2:199551/1‑44 (MQ=255)
                                                                                    cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGa                                                                                                                               <  1:199551/44‑1 (MQ=255)
                                                                                    cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                                       >  1:430424/1‑68 (MQ=255)
                                                                                    cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                                       <  2:430424/68‑1 (MQ=255)
                                                                                         aaGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATa                           <  1:585607/139‑1 (MQ=255)
                                                                                           ggCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACg                         >  1:768904/1‑139 (MQ=255)
                                                                                               tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                                       <  2:1195886/89‑1 (MQ=255)
                                                                                               tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                                       >  1:1195886/1‑89 (MQ=255)
                                                                                               tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCt                                                        <  1:242443/104‑1 (MQ=255)
                                                                                               tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCt                                                        >  2:242443/1‑104 (MQ=255)
                                                                                               tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGccc                     <  1:360004/139‑1 (MQ=255)
                                                                                                      cGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGAt              <  2:768904/139‑1 (MQ=255)
                                                                                                         ttAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                         >  2:88998/1‑45 (MQ=255)
                                                                                                         ttAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                         <  1:88998/45‑1 (MQ=255)
                                                                                                         ttAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCaa           >  1:611115/1‑139 (MQ=255)
                                                                                                                   cccACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTCCAGaa  >  1:581446/1‑139 (MQ=255)
                                                                                                                      |                                                                                                                                       
TGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTCCAGAA  >  NC_000913/3308889‑3309142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: