Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,328,103 A→C intergenic (+20/+136) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,328,1030AC100.0% 164.4 / NA 46intergenic (+20/+136)yhbY/greARNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/26);  total (20/26)

CCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGG  >  NC_000913/3327968‑3328232
                                                                                                                                       |                                                                                                                                 
ccTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCAt                                                                                                                                <  2:744686/139‑1 (MQ=255)
ccTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCAt                                                                                                                                <  1:787057/139‑1 (MQ=255)
      tCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTGATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCtg                                                                                                                          <  2:1280470/139‑1 (MQ=255)
         tGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTg                                                                                                                             >  1:741433/1‑133 (MQ=255)
         tGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTg                                                                                                                             <  2:741433/133‑1 (MQ=255)
                     tGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGt                                                                                                           <  1:239145/139‑1 (MQ=255)
                          gAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTcc                                                                                                      <  1:1396703/139‑1 (MQ=255)
                                           tGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCaaa                                                                                     <  2:333229/139‑1 (MQ=255)
                                               cAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGc                                                                                                                            >  2:710757/1‑96 (MQ=255)
                                               cAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGc                                                                                                                            <  1:710757/96‑1 (MQ=255)
                                                aGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGcc                                                                                >  2:1427122/1‑139 (MQ=255)
                                                  gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCAc                                                                              >  2:1373849/1‑139 (MQ=255)
                                                  gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCAc                                                                              >  1:93344/1‑139 (MQ=255)
                                                   tCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTCAAGAACGTAAAATCTCGCTGCCACGCTTAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACg                                                                             <  1:909463/139‑1 (MQ=255)
                                                     aTCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGct                                                                           <  1:831709/139‑1 (MQ=255)
                                                            aaaCGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCg                                                                    >  2:1191669/1‑139 (MQ=255)
                                                                  tGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATa                                                              >  2:600721/1‑139 (MQ=255)
                                                                   ggTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATaa                                                             <  1:1427122/139‑1 (MQ=255)
                                                                    gTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATaaa                                                            <  2:93344/139‑1 (MQ=255)
                                                                                 cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCg                                               <  1:600721/139‑1 (MQ=255)
                                                                                            cGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCaaa                                                                                     <  1:518640/90‑1 (MQ=255)
                                                                                            cGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCaaa                                                                                     >  2:518640/1‑90 (MQ=255)
                                                                                              tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGtttt                                                                                                        <  1:1411923/69‑1 (MQ=255)
                                                                                              tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGtttt                                                                                                        >  2:1411923/1‑69 (MQ=255)
                                                                                              tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCcttt                                  <  1:1191669/139‑1 (MQ=255)
                                                                                                      cGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGt                                                                       >  1:297769/1‑94 (MQ=255)
                                                                                                      cGCTGCCACGCTAAGATTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGt                                                                       <  2:297769/94‑1 (MQ=255)
                                                                                                                     aTTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCa                                                                               <  1:166378/71‑1 (MQ=255)
                                                                                                                     aTTATCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCa                                                                               >  2:166378/1‑71 (MQ=255)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAg                                                                                                               <  1:65693/35‑1 (MQ=38)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAg                                                                                                               >  2:65693/1‑35 (MQ=38)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCGAAATGcc                                                                                <  2:331693/66‑1 (MQ=255)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGcc                                                                                >  1:661606/1‑66 (MQ=255)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGcc                                                                                <  2:661606/66‑1 (MQ=255)
                                                                                                                         tCCTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGcc                                                                                >  1:331693/1‑66 (MQ=255)
                                                                                                                          ccTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCgcagg                                                                                                 >  1:1297117/1‑48 (MQ=255)
                                                                                                                          ccTAAAGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCgcagg                                                                                                 <  2:1297117/48‑1 (MQ=255)
                                                                                                                             aaaGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATa                        <  1:185408/118‑1 (MQ=255)
                                                                                                                             aaaGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATa                        >  2:185408/1‑118 (MQ=255)
                                                                                                                             aaaGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTgg   <  1:1373849/139‑1 (MQ=255)
                                                                                                                              aaGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTggg  >  2:859044/1‑139 (MQ=255)
                                                                                                                              aaGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTggg  >  1:658167/1‑139 (MQ=255)
                                                                                                                               aGTTACTCCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGga                                                                                            <  1:927440/48‑1 (MQ=255)
                                                                                                                               aGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGga                                                                                            >  2:927440/1‑48 (MQ=255)
                                                                                                                               aGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctc                                                                          >  2:204359/1‑66 (MQ=255)
                                                                                                                               aGTTACACCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctc                                                                          <  1:204359/66‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
CCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGG  >  NC_000913/3327968‑3328232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: