Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,503,952	G→A	*262* (TAG→TAA)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,503,952	0	G	A	100.0%	168.1 / NA	53	*262* (TAG→TAA)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/31);  total (22/31)

ATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAA  >  NC_000913/3503824‑3504081                                                                                                                                 |                                                                                                                                  aTGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGtt                                                                                                                         >  1:1121546/1‑139 (MQ=255)     gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCg                                                                                                                     <  2:375927/139‑1 (MQ=255)          ggAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGc                                                                                                                >  1:106625/1‑139 (MQ=255)               ttCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCtt                                                                                                           >  2:1490980/1‑139 (MQ=255)                tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTc                                                                                                          >  1:94826/1‑139 (MQ=255)                tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTc                                                                                                          >  1:1398304/1‑139 (MQ=255)                   ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                       <  2:1168473/139‑1 (MQ=255)                   ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                       >  1:1395597/1‑139 (MQ=255)                      aTTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                    <  1:320138/139‑1 (MQ=255)                      aTTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                    <  1:1117709/139‑1 (MQ=255)                      aTTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                    <  2:106625/139‑1 (MQ=255)                       ttGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGAGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCaa                                                                                                   <  1:592426/139‑1 (MQ=255)                          ccGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGc                                                                                                                <  2:1184389/123‑1 (MQ=255)                          ccGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGc                                                                                                                >  1:1184389/1‑123 (MQ=255)                           cGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTg                                                                                               <  2:741632/139‑1 (MQ=255)                           cGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTg                                                                                               <  1:761467/139‑1 (MQ=255)                              aTTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTg                                                                                            >  2:532970/1‑139 (MQ=255)                                             gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                       <  1:826242/129‑1 (MQ=255)                                             gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                       >  2:826242/1‑129 (MQ=255)                                             gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                             >  1:338918/1‑139 (MQ=255)                                             gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                             <  1:1490980/139‑1 (MQ=255)                                             gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                             <  1:1118946/139‑1 (MQ=255)                                             gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                             <  2:1231547/139‑1 (MQ=255)                                                tGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                          <  2:751953/139‑1 (MQ=255)                                                       aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGTCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCtata                                                                   >  1:1205729/1‑139 (MQ=255)                                                         gACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAAt                                                                 <  1:1372560/139‑1 (MQ=255)                                                             ttACCGCAGGCGATAGCGCAGGGAACGGCCTGGGCGGCGAAAACCATTTAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTCCACGCCAACGTGCTGTTACTTGCCGGAATACGACCCTATAAGCCGa                                                             <  2:338918/139‑1 (MQ=255)                                                               aCCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTgg                                                                                                                       <  2:1270546/79‑1 (MQ=255)                                                               aCCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTgg                                                                                                                       >  1:1270546/1‑79 (MQ=255)                                                                 cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                          >  2:726099/1‑122 (MQ=255)                                                                 cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                          <  1:726099/122‑1 (MQ=255)                                                                   cAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAatt                                                       >  1:1484978/1‑139 (MQ=255)                                                                     ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                             >  1:1076251/1‑115 (MQ=255)                                                                     ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                             <  2:1076251/115‑1 (MQ=255)                                                                     ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                     <  2:94826/139‑1 (MQ=255)                                                                       cGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                   <  2:1484978/139‑1 (MQ=255)                                                                       cGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                   <  2:1504537/139‑1 (MQ=255)                                                                                  ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                       <  2:1476226/92‑1 (MQ=255)                                                                                  ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                       >  1:1476226/1‑92 (MQ=255)                                                                                       ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                    >  2:1078700/1‑74 (MQ=255)                                                                                       ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                    <  1:1078700/74‑1 (MQ=255)                                                                                            gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCTATCGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                       >  2:879757/1‑130 (MQ=255)                                                                                            gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                          <  1:1352527/95‑1 (MQ=255)                                                                                            gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                          >  2:1352527/1‑95 (MQ=255)                                                                                            gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                       <  1:879757/130‑1 (MQ=255)                                                                                              gcggcgAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                           >  2:510321/1‑92 (MQ=255)                                                                                              gcggcgAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                           <  1:510321/92‑1 (MQ=255)                                                                                              gcggcgAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                            >  1:456612/1‑139 (MQ=255)                                                                                                                        tgtcTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa  <  2:1205729/136‑1 (MQ=255)                                                                                                                        tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                           >  2:96118/1‑66 (MQ=255)                                                                                                                        tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                           <  1:96118/66‑1 (MQ=255)                                                                                                                        tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa  <  2:456612/139‑1 (MQ=255)                                                                                                                        tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa  <  2:1395597/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  ATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAA  >  NC_000913/3503824‑3504081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑