Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 323,355 C→T P340S (CCA→TCA)  ykgF → ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913323,3550CT100.0% 113.9 / NA 37P340S (CCA→TCA) ykgFferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/16);  total (21/16)

GGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTG  >  NC_000913/323227‑323486
                                                                                                                                |                                                                                                                                   
ggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTATATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                             >  1:1215478/1‑137 (MQ=255)
ggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTATATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                             <  2:1215478/137‑1 (MQ=255)
    ttCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTg                                                                                                                       >  1:598856/1‑139 (MQ=255)
                     ctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCAGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                      <  2:892188/139‑1 (MQ=255)
                                      ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                     <  2:598856/139‑1 (MQ=255)
                                              tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                                     <  2:355855/99‑1 (MQ=255)
                                              tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                                     >  1:355855/1‑99 (MQ=255)
                                              tATTCGCTGCGGGGCTTGTATGAATACTTGTCCAGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTa                                                                             >  2:467134/1‑139 (MQ=255)
                                                      gCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAGAGATTTTAAAGATTTACCCTACGc                                                                     >  2:190902/1‑139 (MQ=255)
                                                                      tACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcaca                                                     >  1:936075/1‑139 (MQ=255)
                                                                        cTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                                                   <  2:936075/139‑1 (MQ=255)
                                                                          tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                                                 >  1:81693/1‑139 (MQ=255)
                                                                            tCCAGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgt                                               <  1:467134/139‑1 (MQ=255)
                                                                              cGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGa                                             >  2:1479063/1‑139 (MQ=255)
                                                                                   tatCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                      >  2:137271/1‑77 (MQ=255)
                                                                                   tatCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                      <  1:137271/77‑1 (MQ=255)
                                                                                          atatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc                                 <  1:931087/139‑1 (MQ=255)
                                                                                                gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                                                <  1:226890/86‑1 (MQ=255)
                                                                                                gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                                                >  2:226890/1‑86 (MQ=255)
                                                                                                   gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                                                          >  2:1151375/1‑89 (MQ=255)
                                                                                                   gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                                                          <  1:1151375/89‑1 (MQ=255)
                                                                                                   gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTAt                        >  1:1317102/1‑139 (MQ=255)
                                                                                                      aTGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTcc                     <  1:1479063/139‑1 (MQ=255)
                                                                                                              ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                               >  2:1043172/1‑73 (MQ=255)
                                                                                                              ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                               <  1:1043172/73‑1 (MQ=255)
                                                                                                              ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaa             >  1:1490008/1‑139 (MQ=255)
                                                                                                              ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTACGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaa             >  2:1434109/1‑139 (MQ=255)
                                                                                                                ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaaggttttaaagatt                                                                               >  2:1263760/1‑71 (MQ=255)
                                                                                                                ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaaggttttaaagatt                                                                               <  1:1263760/71‑1 (MQ=255)
                                                                                                                   tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                      <  2:1100832/45‑1 (MQ=255)
                                                                                                                   tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                      >  1:1100832/1‑45 (MQ=255)
                                                                                                                   tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTc               >  1:161982/1‑132 (MQ=255)
                                                                                                                   tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTc               <  2:161982/132‑1 (MQ=255)
                                                                                                                         tCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCt                                                                         >  2:949949/1‑68 (MQ=255)
                                                                                                                         tCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCt                                                                         <  1:949949/68‑1 (MQ=255)
                                                                                                                         tCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTg  >  1:995278/1‑139 (MQ=255)
                                                                                                                             gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                       >  1:1499717/1‑82 (MQ=255)
                                                                                                                                |                                                                                                                                   
GGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTG  >  NC_000913/323227‑323486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: