Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,074	C→T	*257* (TAG→TAA)	bioH ←	pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,074	0	C	T	100.0%	192.9 / NA	59	*257* (TAG→TAA)	bioH	pimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

AGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCA  >  NC_000913/3543940‑3544204                                                                                                                                       |                                                                                                                                   aGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTaca                                                                                                                                <  1:913461/139‑1 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCctc                                                                                                                           >  1:39394/1‑139 (MQ=255)      tGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGCCTCGCCATTTCAAAAGCCACTTACAccccc                                                                                                                           >  1:471207/1‑137 (MQ=255)                  aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                              <  2:391439/124‑1 (MQ=255)                  aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                              >  1:391439/1‑124 (MQ=255)                  aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGcc                                                                                                               <  2:1463539/139‑1 (MQ=255)                  aaaTAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGcc                                                                                                               >  1:1463539/1‑139 (MQ=255)                         gCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGcc                                                                                                               >  2:967161/1‑132 (MQ=255)                         gCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGcc                                                                                                               <  1:967161/132‑1 (MQ=255)                          cTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACcagcag                                                                                                       <  1:760884/139‑1 (MQ=255)                             gAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTg                                                                                                    >  1:1155696/1‑139 (MQ=255)                                     aGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACt                                                                                            >  2:272542/1‑139 (MQ=255)                                         ccTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGc                                                                                        >  1:1417518/1‑139 (MQ=255)                                             ttGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGa                                                                                    <  1:272542/139‑1 (MQ=255)                                              tGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                   <  2:39394/139‑1 (MQ=255)                                                cAGACATCACGCGCTTTACCGGGCTGTCAGAAGATGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGc                                                                                 <  1:966018/139‑1 (MQ=255)                                                   aCATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaa                                                                              >  2:3238/1‑139 (MQ=255)                                                     atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGAc                                                                                                  >  2:697909/1‑117 (MQ=255)                                                     atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGAc                                                                                                  <  1:697909/117‑1 (MQ=255)                                                     atcatGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaat                                                                            >  1:1507246/1‑139 (MQ=255)                                                        atGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaataaa                                                                         <  2:1362130/139‑1 (MQ=255)                                                             cTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGgcg                                                                    >  2:1418657/1‑139 (MQ=255)                                                               ttACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCa                                                                  >  1:1391880/1‑139 (MQ=255)                                                                tACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAAc                                                                                             >  1:135271/1‑111 (MQ=255)                                                                tACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAAc                                                                                             <  2:135271/111‑1 (MQ=255)                                                                 aCCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCa                                                                  <  1:912942/137‑1 (MQ=255)                                                                 aCCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTATGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCa                                                                  >  2:912942/1‑137 (MQ=255)                                                                       cTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                              <  1:671130/71‑1 (MQ=255)                                                                       cTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACAcc                                                                                                                              >  2:671130/1‑71 (MQ=255)                                                                          tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCttt                                                       >  2:151978/1‑139 (MQ=255)                                                                           cAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTg                                                      <  2:471207/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                   <  2:1417518/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                   <  1:3238/139‑1 (MQ=255)                                                                              aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                   <  1:682444/139‑1 (MQ=255)                                                                               agaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGa                                                  <  2:1507246/139‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCa                                                                                                                    <  2:1177110/68‑1 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCa                                                                                                                    >  1:1177110/1‑68 (MQ=255)                                                                                    agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGata                                             >  2:577885/1‑139 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACcagca                                                                                                        <  2:896773/76‑1 (MQ=255)                                                                                        ggCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACcagca                                                                                                        >  1:896773/1‑76 (MQ=255)                                                                                           tGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGAtt                                      >  1:841643/1‑139 (MQ=255)                                                                                           tGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGAtt                                      >  1:707257/1‑139 (MQ=255)                                                                                                 gAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCtgt                                <  1:1418657/139‑1 (MQ=255)                                                                                                      aGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGc                           <  1:577885/139‑1 (MQ=255)                                                                                                             ttCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTg                                                      <  2:1347081/105‑1 (MQ=255)                                                                                                             ttCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTg                                                      >  1:1347081/1‑105 (MQ=255)                                                                                                             ttCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGtt                    >  2:1250402/1‑139 (MQ=255)                                                                                                               cTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaa                                                                             >  2:1162731/1‑80 (MQ=255)                                                                                                               cTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGaaa                                                                             <  1:1162731/80‑1 (MQ=255)                                                                                                               cTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                   >  2:165947/1‑106 (MQ=255)                                                                                                               cTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                   <  1:165947/106‑1 (MQ=255)                                                                                                                 gTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATc                >  1:532222/1‑139 (MQ=255)                                                                                                                    tcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACa                                                                                                 >  1:73350/1‑55 (MQ=255)                                                                                                                    tcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACa                                                                                                 <  2:73350/55‑1 (MQ=255)                                                                                                                       ccATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                   <  1:394676/98‑1 (MQ=255)                                                                                                                       ccATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCg                                                   >  2:394676/1‑98 (MQ=255)                                                                                                                            tCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                   <  1:1226193/61‑1 (MQ=255)                                                                                                                            tCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGAt                                                                                   >  2:1226193/1‑61 (MQ=255)                                                                                                                               aaaGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGcacca  <  1:151978/139‑1 (MQ=255)                                                                                                                               aaaGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGcacca  <  2:841643/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   AGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCA  >  NC_000913/3543940‑3544204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑