Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,598,557	A→G	*368Q (TAA→CAA)	livJ ←	branched‑chain amino acid ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,598,557	0	A	G	100.0%	166.2 / NA	46	*368Q (TAA→CAA)	livJ	branched‑chain amino acid ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/20);  total (26/20)

CAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGG  >  NC_000913/3598425‑3598688                                                                                                                                     |                                                                                                                                    cAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGc                                                                                                                               >  1:1281131/1‑139 (MQ=255) cAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGc                                                                                                                               >  2:593697/1‑139 (MQ=255)        tgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCgg                                                                                                                          >  2:698838/1‑137 (MQ=255)        tgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCgg                                                                                                                          <  1:698838/137‑1 (MQ=255)                         ttCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTg                                                                                                       <  1:593697/139‑1 (MQ=255)                                     tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATAc                                                                                           >  2:1444667/1‑139 (MQ=255)                                           ccGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGaa                                                                                     >  1:658398/1‑139 (MQ=255)                                            cGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCg                                                                                                         >  2:1507574/1‑118 (MQ=255)                                            cGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCg                                                                                                         <  1:1507574/118‑1 (MQ=255)                                               aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCa                                                                                 >  1:851472/1‑139 (MQ=255)                                                       tttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                               <  1:1299011/133‑1 (MQ=255)                                                       tttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                               >  2:1299011/1‑133 (MQ=255)                                                         tACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCa                                                                       >  1:138730/1‑139 (MQ=255)                                                                 aTCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCt                                                               >  2:952586/1‑139 (MQ=255)                                                                  tCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCtt                                                              >  1:1159189/1‑139 (MQ=255)                                                                   ccGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCttt                                                             <  1:1115340/139‑1 (MQ=255)                                                                     ggCATGAAGAAACGTACTCGATATTAGCAATTTTGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                           <  2:745028/139‑1 (MQ=255)                                                                        aTGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCAt                                                        <  1:952586/139‑1 (MQ=255)                                                                           aaGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                               >  2:696362/1‑113 (MQ=255)                                                                           aaGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                               <  1:696362/113‑1 (MQ=255)                                                                            aGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTgg                                                                                                           <  1:952061/84‑1 (MQ=255)                                                                            aGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTgg                                                                                                           >  2:952061/1‑84 (MQ=255)                                                                                aCGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTc                                                <  2:851472/139‑1 (MQ=255)                                                                                 cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCa                                               >  2:563795/1‑139 (MQ=255)                                                                                   tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGc                                             >  2:1478743/1‑139 (MQ=255)                                                                                     cTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                           <  1:1478743/139‑1 (MQ=255)                                                                                           atTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCAt                                     >  2:67319/1‑139 (MQ=255)                                                                                             tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                              >  2:939175/1‑128 (MQ=255)                                                                                             tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGACGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                              <  1:939175/128‑1 (MQ=255)                                                                                              aGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTAc                                  <  2:138730/139‑1 (MQ=255)                                                                                               gCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCg                                                                                       >  2:1295097/1‑85 (MQ=255)                                                                                               gCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCg                                                                                       <  1:1295097/85‑1 (MQ=255)                                                                                                cAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                                <  2:658398/139‑1 (MQ=255)                                                                                                    ttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                           <  2:92781/108‑1 (MQ=255)                                                                                                    ttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                           >  1:92781/1‑108 (MQ=255)                                                                                                    ttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATc                            >  2:332396/1‑139 (MQ=255)                                                                                                    ttGGCGGCAACCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATc                            >  2:809613/1‑139 (MQ=255)                                                                                                             aCCCAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGtt                   >  2:761667/1‑139 (MQ=255)                                                                                                               ccAAAGTTGCCGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCg                 <  1:563795/139‑1 (MQ=255)                                                                                                                        ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGc                                                                                                      <  1:1299028/45‑1 (MQ=255)                                                                                                                        ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGc                                                                                                      >  2:1299028/1‑45 (MQ=255)                                                                                                                        ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGc                                             >  2:581619/1‑102 (MQ=255)                                                                                                                        ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGc                                             <  1:581619/102‑1 (MQ=255)                                                                                                                        ccGATTAATGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGt        >  1:794741/1‑139 (MQ=255)                                                                                                                              aaTGATTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTgg  <  1:332396/139‑1 (MQ=255)                                                                                                                                  aTTGCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                           >  2:230515/1‑94 (MQ=255)                                                                                                                                     |                                                                                                                                    CAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGG  >  NC_000913/3598425‑3598688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑