Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,695,997 C→T *63* (TAG→TAA)  yhjR ← DUF2629 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,695,9970CT100.0% 161.5 / NA 50*63* (TAG→TAA) yhjRDUF2629 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/22);  total (28/22)

TGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695862‑3696132
                                                                                                                                       |                                                                                                                                       
tGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTAc                                                                                                                                      >  1:126791/1‑139 (MQ=255)
     gggCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttg                                                                                                                                 <  2:126791/139‑1 (MQ=255)
     gggCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttg                                                                                                                                 >  1:830355/1‑139 (MQ=255)
      ggcaggcaTCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttgt                                                                                                                                <  2:406968/139‑1 (MQ=255)
          ggcaTCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATTCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            <  1:723285/139‑1 (MQ=255)
                     ccaGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCa                                                                                                                          >  2:555633/1‑130 (MQ=255)
                     ccaGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCa                                                                                                                          <  1:555633/130‑1 (MQ=255)
                                   cccAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            <  2:937911/114‑1 (MQ=255)
                                   cccAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            >  1:937911/1‑114 (MQ=255)
                                                  gACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGCACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            >  1:1326425/1‑99 (MQ=255)
                                                  gACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGCACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            <  2:1326425/99‑1 (MQ=255)
                                                   aCCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   >  1:414659/1‑139 (MQ=255)
                                                    ccAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCa                                                                                  <  1:1214144/139‑1 (MQ=255)
                                                          tAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                            <  2:414659/139‑1 (MQ=255)
                                                                       aTGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAat                                                               >  1:244710/1‑139 (MQ=255)
                                                                            tgttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCa                                                                                                                          <  1:425414/75‑1 (MQ=255)
                                                                            tgttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCa                                                                                                                          >  2:425414/1‑75 (MQ=255)
                                                                              ttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                        >  2:481720/1‑139 (MQ=255)
                                                                               tgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCAt                                                       >  2:1071785/1‑139 (MQ=255)
                                                                                gtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGcc                                                                                         >  1:1488022/1‑104 (MQ=255)
                                                                                gtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGcc                                                                                         <  2:1488022/104‑1 (MQ=255)
                                                                                 tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAata                                                              >  2:1495132/1‑130 (MQ=255)
                                                                                 tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAata                                                              <  1:1495132/130‑1 (MQ=255)
                                                                                 tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATaa                                                     >  2:4131/1‑139 (MQ=255)
                                                                                        gCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                           >  1:1387552/1‑94 (MQ=255)
                                                                                        gCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                           <  2:1387552/94‑1 (MQ=255)
                                                                                           tCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCATCGTTTTAATGCCGCGGCCAACTGtt                                                                            <  2:688153/106‑1 (MQ=255)
                                                                                           tCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                            >  1:688153/1‑106 (MQ=255)
                                                                                             cccGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttgt                                                                                                                                >  1:1347787/1‑52 (MQ=255)
                                                                                             cccGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttgt                                                                                                                                <  2:1347787/52‑1 (MQ=255)
                                                                                                      ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                                  <  1:856207/57‑1 (MQ=255)
                                                                                                      ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                                  >  2:856207/1‑57 (MQ=255)
                                                                                                      ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAAt                                >  2:1005542/1‑139 (MQ=255)
                                                                                                      ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAAt                                >  2:1411976/1‑139 (MQ=255)
                                                                                                       cTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATg                               <  1:1005542/139‑1 (MQ=255)
                                                                                                        tGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcg                                                                          <  2:382316/95‑1 (MQ=255)
                                                                                                        tGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcg                                                                          >  1:382316/1‑95 (MQ=255)
                                                                                                              cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCtt                        >  1:363973/1‑139 (MQ=255)
                                                                                                                 aGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTaa                     <  1:4131/139‑1 (MQ=255)
                                                                                                                       gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCcac               >  2:7244/1‑139 (MQ=255)
                                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                           >  1:783866/1‑62 (MQ=255)
                                                                                                                        ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                           <  2:783866/62‑1 (MQ=255)
                                                                                                                            tCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAatat          <  2:830355/139‑1 (MQ=255)
                                                                                                                             cACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATc         >  1:159443/1‑139 (MQ=255)
                                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCa                                        <  1:624660/103‑1 (MQ=255)
                                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCa                                        >  2:624660/1‑103 (MQ=255)
                                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCa                                        <  1:167809/103‑1 (MQ=255)
                                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCa                                        >  2:167809/1‑103 (MQ=255)
                                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCt    >  1:413245/1‑139 (MQ=255)
                                                                                                                                  aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATAACATTCt    >  2:423583/1‑139 (MQ=255)
                                                                                                                                    ccctTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg  <  2:159443/139‑1 (MQ=255)
                                                                                                                                    ccctTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg  <  1:7244/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695862‑3696132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑