Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,731,980 G→A G284S (GGT→AGT)  xylF → D‑xylose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,9800GA100.0% 185.7 / NA 57G284S (GGT→AGT) xylFD‑xylose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/30);  total (27/30)

GTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGA  >  NC_000913/3731845‑3732112
                                                                                                                                       |                                                                                                                                    
gTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTCAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGCTGGCAAATACTGCAGCAGAAATTGGAGTTGAGTTGGGCAATAGTc                                                                                                                                   >  2:1215551/1‑139 (MQ=255)
        ctcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                           <  2:428019/139‑1 (MQ=255)
         tcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                          >  2:338661/1‑139 (MQ=255)
         tcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                          <  1:757159/139‑1 (MQ=255)
             gCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                      <  1:338661/139‑1 (MQ=255)
                            tcGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                 >  1:1431256/1‑129 (MQ=255)
                            tcGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                 <  2:1431256/129‑1 (MQ=255)
                                          cGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAg                                                                                         <  2:854043/139‑1 (MQ=255)
                                            tATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                                 >  1:345953/1‑97 (MQ=255)
                                            tATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                                 <  2:345953/97‑1 (MQ=255)
                                             aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATg                                                                                      >  2:477860/1‑139 (MQ=255)
                                                       gTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                            <  1:477860/139‑1 (MQ=255)
                                                         aCGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                              <  1:1285785/103‑1 (MQ=255)
                                                         aCGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                              >  2:1285785/1‑103 (MQ=255)
                                                                        gtgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                 <  2:311823/85‑1 (MQ=255)
                                                                        gtgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                 >  1:311823/1‑85 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCa                                                                                                                                  <  2:1098741/67‑1 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCa                                                                                                                                  >  1:1098741/1‑67 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaata                                                                                                     >  1:89573/1‑96 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaata                                                                                                     <  2:89573/96‑1 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                            >  2:1295701/1‑121 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                            <  1:1295701/121‑1 (MQ=255)
                                                                           tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                        <  1:742540/139‑1 (MQ=255)
                                                                             taAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtgtg                                                      >  1:1464607/1‑139 (MQ=255)
                                                                                   tATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                              <  2:1506209/77‑1 (MQ=255)
                                                                                   tATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                              >  1:1506209/1‑77 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaaaa                                                                                                                        <  1:1387452/65‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaaaa                                                                                                                        >  2:1387452/1‑65 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGcc                                                                                               <  1:1054840/90‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGcc                                                                                               >  2:1054840/1‑90 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                            >  1:242863/1‑109 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                            <  2:335880/109‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                            <  2:242863/109‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                            >  1:335880/1‑109 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                              >  2:115144/1‑139 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                              >  2:74605/1‑139 (MQ=255)
                                                                                      tACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaac                                             <  1:115144/139‑1 (MQ=255)
                                                                                           tgttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACAt                                        <  1:74605/139‑1 (MQ=255)
                                                                                             ttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCa                                      <  2:788696/139‑1 (MQ=255)
                                                                                               ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCaaa                                    >  1:1427237/1‑139 (MQ=255)
                                                                                                    aTACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATAc                               <  2:1427237/139‑1 (MQ=255)
                                                                                                      aCTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACgg                             <  1:712315/139‑1 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                           <  2:1460581/44‑1 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                           >  1:1460581/1‑44 (MQ=255)
                                                                                                          ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                 <  2:274210/51‑1 (MQ=255)
                                                                                                          ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                 >  1:274210/1‑51 (MQ=255)
                                                                                                          ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaa                                                >  1:447938/1‑116 (MQ=255)
                                                                                                          ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaa                                                <  2:447938/116‑1 (MQ=255)
                                                                                                            gCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaac                                             >  2:1076387/1‑117 (MQ=255)
                                                                                                            gCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaac                                             <  1:1076387/117‑1 (MQ=255)
                                                                                                             cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataa                                                                                                    <  2:588425/61‑1 (MQ=255)
                                                                                                             cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataa                                                                                                    >  1:588425/1‑61 (MQ=255)
                                                                                                                          tGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcga                                                              >  2:242495/1‑86 (MQ=255)
                                                                                                                          tGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcga                                                              <  1:242495/86‑1 (MQ=255)
                                                                                                                            aGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTcccc                                                                                 <  2:1259139/65‑1 (MQ=255)
                                                                                                                            aGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTcccc                                                                                 >  1:1259139/1‑65 (MQ=255)
                                                                                                                                 ggCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAagaga  >  1:755936/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGA  >  NC_000913/3731845‑3732112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑