Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,810,304	G→A	*160* (TAG→TAA)	coaD →	pantetheine‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,810,304	0	G	A	100.0%	141.5 / NA	44	*160* (TAG→TAA)	coaD	pantetheine‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

AGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTC  >  NC_000913/3810192‑3810429                                                                                                                 |                                                                                                                              agTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATcc                                                                                                     >  2:623208/1‑139 (MQ=255)   tGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCgg                                                                                                   >  1:523731/1‑139 (MQ=255)   tGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCgg                                                                                                   <  1:623208/139‑1 (MQ=255)    ggTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa                                                                                                                  <  2:313414/123‑1 (MQ=255)    ggTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGa                                                                                                                  >  1:313414/1‑123 (MQ=255)    ggTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGAt                                                                                                                 <  1:1501876/124‑1 (MQ=255)    ggTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGAt                                                                                                                 >  2:1501876/1‑124 (MQ=255)     gTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcg                                                                                                 >  1:1161968/1‑139 (MQ=255)     gTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcg                                                                                                 >  2:779464/1‑139 (MQ=255)         tttATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCa                                                                                             <  1:779464/139‑1 (MQ=255)         tttATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCa                                                                                             <  2:523731/139‑1 (MQ=255)                 ttCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTAc                                                                                     >  1:802472/1‑139 (MQ=255)                           gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGttt                                                                                                                          <  1:65270/92‑1 (MQ=255)                           gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGttt                                                                                                                          >  2:65270/1‑92 (MQ=255)                           gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTa                                                                                                                         <  1:1178385/93‑1 (MQ=255)                           gTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTa                                                                                                                         >  2:1178385/1‑93 (MQ=255)                                agagGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGa                                                                      >  1:1243885/1‑139 (MQ=255)                                                       aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATaaa                                                               <  1:410886/123‑1 (MQ=255)                                                       aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATaaa                                                               >  2:410886/1‑123 (MQ=255)                                                       aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTgcgc                                               >  2:554561/1‑139 (MQ=255)                                                       aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTgc                                                 >  1:851322/1‑137 (MQ=255)                                                       aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTgc                                                 <  2:851322/137‑1 (MQ=255)                                                        tGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgcgc                                                                                              <  1:652242/91‑1 (MQ=255)                                                        tGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgcgc                                                                                              >  2:652242/1‑91 (MQ=255)                                                                  ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg                                                                                                                    >  2:617231/1‑59 (MQ=255)                                                                  ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCg                                                                                                                    <  1:617231/59‑1 (MQ=255)                                                                  ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAAt                                                                  <  2:284617/109‑1 (MQ=255)                                                                  ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAAt                                                                  >  1:284617/1‑109 (MQ=255)                                                                      tGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCa                                                                                             >  2:759971/1‑78 (MQ=255)                                                                      tGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCa                                                                                             <  1:759971/78‑1 (MQ=255)                                                                        ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgc                                                                                                  <  1:686547/71‑1 (MQ=255)                                                                        ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgc                                                                                                  >  2:686547/1‑71 (MQ=255)                                                                           gagaATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCg                           <  2:1243885/139‑1 (MQ=255)                                                                                  tCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATg                                                                                                                       >  2:1186760/1‑40 (MQ=255)                                                                                  tCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATg                                                                                                                       <  1:1186760/40‑1 (MQ=255)                                                                                  tCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgcg                                                                                               >  1:937901/1‑64 (MQ=255)                                                                                  tCCATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGgcgcg                                                                                               <  2:937901/64‑1 (MQ=255)                                                                                    cATCAGGCGCTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGc                  <  1:660703/139‑1 (MQ=255)                                                                                            gcTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                        >  1:765560/1‑61 (MQ=255)                                                                                            gcTGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                        <  2:765560/61‑1 (MQ=255)                                                                                              tGATGGCGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGaca        <  2:740181/139‑1 (MQ=255)                                                                                                    cGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTc  >  2:552785/1‑139 (MQ=255)                                                                                                    cGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTc  >  2:428356/1‑139 (MQ=255)                                                                                                    cGAAGTTAGCGTAACGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTc  <  2:802472/139‑1 (MQ=255)                                                                                                                 |                                                                                                                              AGTGGTCGTTTATCTCTTCATCGTTGGTGAAAGAGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGACACGGCTC  >  NC_000913/3810192‑3810429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑