Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,991	G→A	*272* (TAG→TAA)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,991	0	G	A	100.0%	237.4 / NA	73	*272* (TAG→TAA)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/35);  total (38/35)

GCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTG  >  NC_000913/344859‑345118                                                                                                                                     |                                                                                                                                gCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                           <  2:1145866/139‑1 (MQ=255)     tctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                          >  1:367653/1‑136 (MQ=255)     tctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                          <  2:367653/136‑1 (MQ=255)      ctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAggg                                                                                                                      >  2:356323/1‑139 (MQ=255)            tGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                    <  1:1256494/135‑1 (MQ=255)            tGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                    >  2:1256494/1‑135 (MQ=255)                 gTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                           >  1:870459/1‑139 (MQ=255)                   aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                          >  2:191323/1‑122 (MQ=255)                   aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                          <  1:191323/122‑1 (MQ=255)                       aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                     <  2:870459/139‑1 (MQ=255)                            cAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                >  2:428343/1‑139 (MQ=255)                                gatgatGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                                                                                            <  1:356323/139‑1 (MQ=255)                                   gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                          <  2:257008/106‑1 (MQ=255)                                   gatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                          >  1:257008/1‑106 (MQ=255)                                    atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                     >  2:1403130/1‑126 (MQ=255)                                    atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                     <  1:1403130/126‑1 (MQ=255)                                         cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGa                                                                                   >  2:340935/1‑139 (MQ=255)                                             tAAATGAATGGATAGCTAATCGAGTGCAGGAATTAGGTGACATATATTATCCTGTAAATAAGCTTGTTAACAAACGCTACGCTCTTTAATTCAGAAAGGGTGTCACCTGAAAGTGGTTATTCCACCTGGAAGTGAATCg                                                                               <  2:954700/139‑1 (MQ=255)                                                        atatCTAATAGAGTGCAGGAATTAGGTGAGAGATATTATCCTGAAAATAAGCACGTTAAAATCCGCTACACTCTATAATTCAGAAAGGGTTCCACCTGAAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                    >  1:1008098/1‑139 (MQ=255)                                                                  gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGATTCGCAATg                                                                          >  2:928301/1‑123 (MQ=255)                                                                  gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATg                                                                          <  1:928301/123‑1 (MQ=255)                                                                   agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                           <  1:1126041/73‑1 (MQ=255)                                                                   agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                           >  2:1126041/1‑73 (MQ=255)                                                                     tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCATAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGc                                                                         <  2:1375646/121‑1 (MQ=255)                                                                     tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTCCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGc                                                                         >  1:1375646/1‑121 (MQ=255)                                                                     tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGATAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                 <  2:1300146/97‑1 (MQ=255)                                                                     tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGATAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                 >  1:1300146/1‑97 (MQ=255)                                                                      gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                      >  2:1438127/1‑139 (MQ=255)                                                                       cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                     >  2:851518/1‑139 (MQ=255)                                                                          gAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtatat                                                  <  1:428343/139‑1 (MQ=255)                                                                                   gACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                         <  1:851518/139‑1 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                            <  1:1087385/88‑1 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                            >  2:1087385/1‑88 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAg                                                                       >  1:424607/1‑93 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAg                                                                       <  2:424607/93‑1 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                     >  2:1285115/1‑111 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                     <  1:1285115/111‑1 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCAt                                >  1:1211908/1‑132 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCAt                                <  2:1211908/132‑1 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                         >  1:1491489/1‑139 (MQ=255)                                                                                                   tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                         >  2:1015931/1‑139 (MQ=255)                                                                                                     gaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                    >  2:322216/2‑94 (MQ=255)                                                                                                     gaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                    <  1:322216/93‑1 (MQ=255)                                                                                                     aaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                    >  1:1281854/1‑46 (MQ=255)                                                                                                     aaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                    <  2:1281854/46‑1 (MQ=255)                                                                                                     aaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATctct                       >  2:1339701/1‑139 (MQ=255)                                                                                                        aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                         >  1:127860/1‑118 (MQ=255)                                                                                                        aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                         <  2:127860/118‑1 (MQ=255)                                                                                                          gCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                 <  1:1321866/76‑1 (MQ=255)                                                                                                          gCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                 >  2:1321866/1‑76 (MQ=255)                                                                                                          gCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                    >  2:236071/1‑105 (MQ=255)                                                                                                          gCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                    <  1:236071/105‑1 (MQ=255)                                                                                                          gCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttttt                  <  2:1491489/139‑1 (MQ=255)                                                                                                             cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                           <  2:370718/47‑1 (MQ=255)                                                                                                             cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                           >  1:370718/1‑47 (MQ=255)                                                                                                             cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                 >  2:1160824/1‑57 (MQ=255)                                                                                                             cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                 <  1:1160824/57‑1 (MQ=255)                                                                                                               ttAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCg             >  2:1179028/1‑139 (MQ=255)                                                                                                                  aaaTCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAAt                                                                           <  1:970350/74‑1 (MQ=255)                                                                                                                  aaaGCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAAt                                                                           >  2:970350/1‑74 (MQ=255)                                                                                                                      ccGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                     >  2:1252483/1‑44 (MQ=255)                                                                                                                      ccGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                     >  2:1033235/1‑44 (MQ=255)                                                                                                                      ccGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                     <  1:1252483/44‑1 (MQ=255)                                                                                                                      ccGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                     <  1:1033235/44‑1 (MQ=255)                                                                                                                        gCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                     >  2:522672/1‑90 (MQ=255)                                                                                                                        gCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                     <  1:522672/90‑1 (MQ=255)                                                                                                                          tACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                         <  1:967852/52‑1 (MQ=255)                                                                                                                          tACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                         >  2:572677/1‑52 (MQ=255)                                                                                                                          tACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                         <  1:572677/52‑1 (MQ=255)                                                                                                                          tACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                                                                                         >  2:967852/1‑52 (MQ=255)                                                                                                                          tACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgttg  <  1:97699/139‑1 (MQ=255)                                                                                                                                 tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa                                        <  2:761227/94‑1 (MQ=255)                                                                                                                                 tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa                                        >  1:761227/1‑94 (MQ=255)                                                                                                                                     |                                                                                                                                GCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTG  >  NC_000913/344859‑345118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑