Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,838,137	G→A	*395* (TAG→TAA)	setC →	putative arabinose efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,838,137	0	G	A	100.0%	121.2 / NA	39	*395* (TAG→TAA)	setC	putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/22);  total (17/22)

GGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGACAAAACAGTATGCAGAGGGAAAAATGGGTTCCACCAGAAAGG  >  NC_000913/3838009‑3838266                                                                                                                                 |                                                                                                                                  ggCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTc                                                                                                                         >  2:932671/1‑139 (MQ=255) ggCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTc                                                                                                                         <  1:52647/139‑1 (MQ=255)                ggCTCTGTTGACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAAt                                                                                                          >  1:1077846/1‑139 (MQ=255)                         gACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGgc                                                                                                 <  2:1077846/139‑1 (MQ=255)                          aCGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGgcg                                                                                                <  1:932671/139‑1 (MQ=255)                            ggAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCa                                                                                              >  1:281805/1‑139 (MQ=255)                                                      ccATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGc                                                                    >  2:867181/1‑139 (MQ=255)                                                             ttgttCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCg                                                                                                     <  2:997498/99‑1 (MQ=255)                                                             ttgttCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCg                                                                                                     >  1:997498/1‑99 (MQ=255)                                                             ttgttCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATg                                                             <  1:1228639/139‑1 (MQ=255)                                                              tgttCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGc                                                            <  2:476912/139‑1 (MQ=255)                                                                ttCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCt                                                          <  1:867181/139‑1 (MQ=255)                                                                         gcGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGcatacata                                                 <  1:1135286/139‑1 (MQ=255)                                                                          cGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATaa                                                >  2:129027/1‑139 (MQ=255)                                                                           gATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATaaa                                               <  2:281805/139‑1 (MQ=255)                                                                              aGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGa                                            <  2:1411401/139‑1 (MQ=255)                                                                                ggATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGgcg                                                                                                >  1:323646/1‑85 (MQ=255)                                                                                ggATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGgcg                                                                                                <  2:323646/85‑1 (MQ=255)                                                                                    gTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGACAAAAc                                      <  1:129027/139‑1 (MQ=255)                                                                                            ttGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTg                                                                     <  1:1510240/100‑1 (MQ=255)                                                                                            ttGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTg                                                                     >  2:1510240/1‑100 (MQ=255)                                                                                             tGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCa                                                                                                                               >  1:230362/1‑41 (MQ=255)                                                                                             tGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAACTCa                                                                                                                               <  2:230362/41‑1 (MQ=255)                                                                                                  tgatTTGCCTGCTGTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGACAAAACAGTATGCAGAGGGa                        >  1:923706/1‑139 (MQ=255)                                                                                                              tgTTTATTAAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGACAAAACAGTATGCAGAGGGAAAAATGGGTTcc            >  1:269154/1‑139 (MQ=255)                                                                                                                  tattaAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCt                                                                                >  2:216315/1‑67 (MQ=255)                                                                                                                  tattaAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCt                                                                                <  1:216315/67‑1 (MQ=255)                                                                                                                    ttaAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCAttttt                                                                      >  1:569365/1‑75 (MQ=255)                                                                                                                    ttaAAGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCAttttt                                                                      <  2:569365/75‑1 (MQ=255)                                                                                                                        aGATATTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGACAAAACAGTATGCAGAGGGAAAAATGGGTTCCACCAGAAAgg  <  2:927185/139‑1 (MQ=255)                                                                                                                          atatTTAACTCACCAGTCTGGGCTAAGAAAAATCGGc                                                                                                      <  2:13805/37‑1 (MQ=255)                                                                                                                          atatTTAACTCACCAGTCTGGGCTAAGAAAAATCGGc                                                                                                      >  1:13805/1‑37 (MQ=255)                                                                                                                          atatTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGTGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATaaa                                               >  2:310091/1‑92 (MQ=255)                                                                                                                          atatTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGTGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATaaa                                               <  1:310091/92‑1 (MQ=255)                                                                                                                          atatTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTc                                                                               <  2:274331/60‑1 (MQ=255)                                                                                                                          atatTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTc                                                                               >  1:274331/1‑60 (MQ=255)                                                                                                                            atTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCt                                                                                <  2:1186772/57‑1 (MQ=255)                                                                                                                            atTTAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCt                                                                                >  1:1186772/1‑57 (MQ=255)                                                                                                                              ttAACTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGACAAAACAGTATGCAGAGGGAAAAATGGGTTccac          <  1:176287/125‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  GGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGAGCCATCCTCGCCATTTTTGCTATCATGCCTGCATACATAAACGACAAAACAGTATGCAGAGGGAAAAATGGGTTCCACCAGAAAGG  >  NC_000913/3838009‑3838266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑