Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,054,439 A→G T280T (ACT→ACC glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,054,4390AG100.0% 114.1 / NA 33T280T (ACT→ACCglnGfused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

AAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATA  >  NC_000913/4054305‑4054572
                                                                                                                                      |                                                                                                                                     
aaaTGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGgtgggtgg                                                                                                                                   <  2:1486791/139‑1 (MQ=255)
         gcCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATaa                                                                                                                          <  2:482014/139‑1 (MQ=255)
         gcCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATaa                                                                                                                          <  2:1298311/139‑1 (MQ=255)
             gACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCGTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCg                                                                                                                      <  2:1067077/139‑1 (MQ=255)
             gACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCg                                                                                                                      <  2:1352692/139‑1 (MQ=255)
                            cccGACGTTCGCACAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCAc                                                                                                       >  2:1327603/1‑139 (MQ=255)
                              cGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAg                                                                                                                                >  2:896689/1‑112 (MQ=255)
                              cGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAg                                                                                                                                <  1:896689/112‑1 (MQ=255)
                              cGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCg                                                                                                     >  1:627788/1‑139 (MQ=255)
                                aCGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGgc                                                                                                   >  2:1347868/1‑139 (MQ=255)
                                 cGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCAc                                                                                                       >  2:431997/1‑134 (MQ=255)
                                 cGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCAc                                                                                                       <  1:431997/134‑1 (MQ=255)
                                 cGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGgcg                                                                                                  >  2:766128/1‑139 (MQ=255)
                                  gTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGgcgc                                                                                                 >  1:1090385/1‑139 (MQ=255)
                                   ttCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCa                                                                                                <  2:627788/139‑1 (MQ=255)
                                       cgcAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGgc                                                                                                   <  1:592811/132‑1 (MQ=255)
                                       cgcAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGgc                                                                                                   >  2:592811/1‑132 (MQ=255)
                                             ggcggcAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCaa                                                                                      <  2:1090385/139‑1 (MQ=255)
                                                          cgcgGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGgtgggtgg                                                                                                                                   <  2:1242975/81‑1 (MQ=255)
                                                          cgcgGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGgtgggtgg                                                                                                                                   >  1:1242975/1‑81 (MQ=255)
                                                          cgcgGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAAcg                                                                                    >  1:387352/1‑128 (MQ=255)
                                                          cgcgGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAAcg                                                                                    <  2:387352/128‑1 (MQ=255)
                                                           gcgGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGa                                                                        >  1:385049/1‑139 (MQ=255)
                                                             ggATAACGTTCACGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGAcc                                                                      <  2:900610/139‑1 (MQ=255)
                                                                            ggTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGACAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCAcgcg                                                       <  2:1039679/139‑1 (MQ=255)
                                                                                gAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGgtgggtgg                                                                                                                                   >  1:1054668/1‑59 (MQ=255)
                                                                                gAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGgtgggtgg                                                                                                                                   <  2:1054668/59‑1 (MQ=255)
                                                                                 aaCAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCgcagca                                                  <  1:766128/139‑1 (MQ=255)
                                                                                    aGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAAc                                               >  1:1459111/1‑139 (MQ=255)
                                                                                      aTCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGAc                                                                       <  2:1404502/113‑1 (MQ=255)
                                                                                      aTCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGAc                                                                       >  1:1404502/1‑113 (MQ=255)
                                                                                        ccTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGcaca                                                                                                                  <  2:387756/68‑1 (MQ=255)
                                                                                        ccTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGcaca                                                                                                                  >  1:387756/1‑68 (MQ=255)
                                                                                                             gCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAAt                                                                                                                         <  2:799238/40‑1 (MQ=255)
                                                                                                             gCACTCGCTGTTCGAGATTCTGGTGGGTGGCAGCGATAAt                                                                                                                         >  1:799238/1‑40 (MQ=255)
                                                                                                                   gCTGTTCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAAt                >  2:1482016/1‑139 (MQ=255)
                                                                                                                       ttCGAGATTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTtcg            <  1:1347868/139‑1 (MQ=255)
                                                                                                                            gaTTCTGGTGGGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAg       <  1:1482016/139‑1 (MQ=255)
                                                                                                                                 tggtggGTGGCAGCGATAATCCGCACATCCACTTTCAc                                                                                                       >  2:118793/1‑38 (MQ=255)
                                                                                                                                 tggtggGTGGCAGCGATAATCCGCACATCCACTTTCAc                                                                                                       <  1:118793/38‑1 (MQ=255)
                                                                                                                                 tggtggGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaata  >  1:393297/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
AAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATA  >  NC_000913/4054305‑4054572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: