Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,229	A→G	S143S (AGT→AGC)	yijF ←	DUF1287 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,229	0	A	G	100.0%	220.1 / NA	60	S143S (AGT→AGC)	yijF	DUF1287 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/31);  total (29/31)

CTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAA  >  NC_000913/4137093‑4137358                                                                                                                                         |                                                                                                                                  cttcttCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCt                                                                                                                                 >  2:708844/1‑139 (MQ=255)    cttcCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTggg                                                                                                                              >  1:372703/1‑139 (MQ=255)           cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTg                                                                                                                       >  2:689742/1‑139 (MQ=255)           cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTg                                                                                                                       >  2:1415098/1‑139 (MQ=255)            gcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGc                                                                                                                      <  1:708844/139‑1 (MQ=255)                    gcCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                              <  2:612628/139‑1 (MQ=255)                          gTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGtt                                                                                                        <  1:1415098/139‑1 (MQ=255)                               gTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTgggg                                                                                                                             >  2:542819/1‑113 (MQ=255)                               gTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTgggg                                                                                                                             <  1:542819/113‑1 (MQ=255)                                 aTCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCAt                                                                                                 <  2:372703/139‑1 (MQ=255)                                  tCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                <  1:598627/139‑1 (MQ=255)                                  tCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                <  1:1217849/139‑1 (MQ=255)                                     ccAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                             <  1:1010416/123‑1 (MQ=255)                                     ccAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                             >  2:1010416/1‑123 (MQ=255)                                      cAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcgg                                                                                            <  1:217184/139‑1 (MQ=255)                                       aGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATTGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcggc                                                                                           <  2:1065885/139‑1 (MQ=255)                                            cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcggc                                                                                           >  2:734530/1‑134 (MQ=255)                                            cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcggc                                                                                           <  1:734530/134‑1 (MQ=255)                                            cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAc                                                                                      >  1:250688/1‑139 (MQ=255)                                                   tCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCg                                                                                                            >  2:1195081/1‑110 (MQ=255)                                                   tCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCg                                                                                                            <  1:1195081/110‑1 (MQ=255)                                                   tCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGttt                                                                               <  2:1055413/139‑1 (MQ=255)                                                        ggCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTggg                                                                                                               >  1:833341/1‑102 (MQ=255)                                                        ggCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTggg                                                                                                               <  2:833341/102‑1 (MQ=255)                                                             aGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTg                                                                                                                                >  2:1316392/1‑80 (MQ=255)                                                             aGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTg                                                                                                                                <  1:1316392/80‑1 (MQ=255)                                                                   cTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCg                                                               >  2:1124145/1‑139 (MQ=255)                                                                    tGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGa                                                              <  1:1124145/139‑1 (MQ=255)                                                                      aaaCCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACg                                                            <  2:250688/139‑1 (MQ=255)                                                                            ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATc                                                      >  1:630809/1‑139 (MQ=255)                                                                            ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATc                                                      >  1:1391175/1‑139 (MQ=255)                                                                              ccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGa                                                    <  1:689742/139‑1 (MQ=255)                                                                                       gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                              <  1:1204647/72‑1 (MQ=255)                                                                                       gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                              >  2:1204647/1‑72 (MQ=255)                                                                                       gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGt                                           >  2:326703/1‑139 (MQ=255)                                                                                          aGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCg                                        <  2:1391175/139‑1 (MQ=255)                                                                                           gCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCgg                                       >  2:1040214/1‑139 (MQ=255)                                                                                            cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                <  1:1298200/81‑1 (MQ=255)                                                                                            cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                >  2:1298200/1‑81 (MQ=255)                                                                                                ttATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCt                                                                                                                                 >  1:557869/1‑44 (MQ=255)                                                                                                ttATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCg                                                                                                            <  1:1507105/65‑1 (MQ=255)                                                                                                ttATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCg                                                                                                            >  2:1507105/1‑65 (MQ=255)                                                                                                ttATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCt                                  >  2:1181635/1‑139 (MQ=255)                                                                                                 tATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCtt                                 <  2:10319/139‑1 (MQ=255)                                                                                                 tATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCtt                                 <  1:1181635/139‑1 (MQ=255)                                                                                                       gTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTcc                                         >  1:273831/1‑125 (MQ=255)                                                                                                       gTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTcc                                         <  2:273831/125‑1 (MQ=255)                                                                                                          gCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCa                        >  2:661760/1‑139 (MQ=255)                                                                                                            cAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCAtt                      <  1:661760/139‑1 (MQ=255)                                                                                                             aGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCAttt                     >  1:92575/1‑139 (MQ=255)                                                                                                              ggAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCAtttt                    <  1:1040214/139‑1 (MQ=255)                                                                                                                    aaTATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                              <  2:144876/43‑1 (MQ=255)                                                                                                                    aaTATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                              >  1:144876/1‑43 (MQ=255)                                                                                                                    aaTATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCggg              >  2:206132/1‑139 (MQ=255)                                                                                                                      tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTa            <  2:630809/139‑1 (MQ=255)                                                                                                                           cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAg       >  1:1382590/1‑139 (MQ=255)                                                                                                                              gCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATCAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCaa    <  1:993291/139‑1 (MQ=255)                                                                                                                               cTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcggc                                                                                           <  2:549009/51‑1 (MQ=255)                                                                                                                               cTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcggc                                                                                           >  1:549009/1‑51 (MQ=255)                                                                                                                                ttGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCaaaa  >  2:1282645/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                  CTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAA  >  NC_000913/4137093‑4137358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑