Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,204,645 G→A *442* (TAG→TAA
N429N (AAC→AAT
zraR →
purD ←
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,204,6450GA100.0% 140.4 / NA 44*442* (TAG→TAA
N429N (AAC→AAT
zraR
purD
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

CAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCAC  >  NC_000913/4204517‑4204777
                                                                                                                                |                                                                                                                                    
cAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTcgc                                                                                                                            >  1:916990/1‑139 (MQ=255)
  gCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGt                                                                                                                          >  2:434362/1‑139 (MQ=255)
                  gaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAg                                                                                                                   <  2:965092/130‑1 (MQ=255)
                  gaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAg                                                                                                                   >  1:965092/1‑130 (MQ=255)
                   aaaaaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGcc                                                                                                         >  1:392366/1‑139 (MQ=255)
                          ggTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCt                                                                                                  >  2:398453/1‑139 (MQ=255)
                              aTTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcg                                                                                                               >  2:998709/1‑122 (MQ=255)
                              aTTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcg                                                                                                               <  1:998709/122‑1 (MQ=255)
                                       gcgcTGGAGAAAACGGGCGGCAACAAAACTGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGATAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGc                                                                                     <  1:1018596/139‑1 (MQ=255)
                                         gcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAg                                                                                   <  2:392366/139‑1 (MQ=255)
                                         gcTGGAGAAAACGAGCGGCAACAAAACCGAAGCCGCCCGTAAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATAGATTTCCGACAGAAGCAg                                                                                   >  2:1018596/1‑139 (MQ=255)
                                              agaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                              <  2:337062/139‑1 (MQ=255)
                                                  aaCGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACAGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAg                                                                          >  1:937736/1‑139 (MQ=255)
                                                         ggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGatat                                                                   <  2:937736/139‑1 (MQ=255)
                                                                                       gggATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGCATTAAGGCATAGGCGCGTTTCTgcgc                                    >  2:1243447/1‑139 (MQ=255)
                                                                                            cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                              >  1:867733/1‑93 (MQ=255)
                                                                                            cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                              <  2:867733/93‑1 (MQ=255)
                                                                                              cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTg                                                                                                                                 <  1:1405607/40‑1 (MQ=255)
                                                                                              cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTg                                                                                                                                 >  2:1405607/1‑40 (MQ=255)
                                                                                              cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgtgc                                                                                                              <  2:873502/59‑1 (MQ=255)
                                                                                              cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAgcgcgc                                                                                                              >  1:873502/1‑59 (MQ=255)
                                                                                              cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGaa                                                                                       <  1:941970/82‑1 (MQ=255)
                                                                                              cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGaa                                                                                       >  2:941970/1‑82 (MQ=255)
                                                                                              cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                              >  1:412740/1‑139 (MQ=255)
                                                                                                          tATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAcc                  >  1:717757/1‑139 (MQ=255)
                                                                                                           aTTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                  <  1:917181/74‑1 (MQ=255)
                                                                                                           aTTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                  >  2:917181/1‑74 (MQ=255)
                                                                                                               gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                             <  2:448374/75‑1 (MQ=255)
                                                                                                               gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                             >  1:448374/1‑75 (MQ=255)
                                                                                                               gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                 >  2:244867/1‑87 (MQ=255)
                                                                                                               gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                 <  1:244867/87‑1 (MQ=255)
                                                                                                               gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg             <  2:412740/139‑1 (MQ=255)
                                                                                                                  aaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGc                                                                                                          >  1:847360/1‑43 (MQ=255)
                                                                                                                  aaaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGc                                                                                                          <  2:847360/43‑1 (MQ=255)
                                                                                                                   aaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGc                                                                                                          <  1:1392796/42‑1 (MQ=255)
                                                                                                                   aaCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGc                                                                                                          >  2:1392796/1‑42 (MQ=255)
                                                                                                                    aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat                                                                                                      >  2:322147/1‑45 (MQ=255)
                                                                                                                    aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat                                                                                                      <  1:322147/45‑1 (MQ=255)
                                                                                                                    aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat                                                                                                      <  2:521197/45‑1 (MQ=255)
                                                                                                                    aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat                                                                                                      >  1:521197/1‑45 (MQ=255)
                                                                                                                        tCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                 <  2:791728/78‑1 (MQ=255)
                                                                                                                        tCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                 >  1:791728/1‑78 (MQ=255)
                                                                                                                          gcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGcac  <  2:717757/139‑1 (MQ=255)
                                                                                                                          gcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGcac  <  1:434362/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
CAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCAC  >  NC_000913/4204517‑4204777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: