Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,297,668 C→T D567N (GAC→AAC)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,297,6680CT100.0% 189.0 / NA 58D567N (GAC→AAC) fdhFformate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/26);  total (32/26)

TATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCC  >  NC_000913/4297533‑4297797
                                                                                                                                       |                                                                                                                                 
tATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGAt                                                                                                                                <  1:1479962/139‑1 (MQ=255)
         gtgCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTAc                                                                                                                       <  2:1474255/139‑1 (MQ=255)
               aGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTc                                                                                                                 >  1:1219447/1‑139 (MQ=255)
                          tcaGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaaga                                                                                                      >  2:15654/1‑139 (MQ=255)
                                          gTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGtt                                                                                      <  2:148955/139‑1 (MQ=255)
                                            gCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                    >  1:1225817/1‑139 (MQ=255)
                                                 acaGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTa                                                                               >  1:788924/1‑139 (MQ=255)
                                                     ttACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCaa                                                                           >  2:54468/1‑139 (MQ=255)
                                                        ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                        <  2:1219447/139‑1 (MQ=255)
                                                             cATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTctct                                                                   <  1:54468/139‑1 (MQ=255)
                                                               tCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCttt                                                                 <  1:189729/139‑1 (MQ=255)
                                                               tCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGGGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCttt                                                                 >  1:825096/1‑139 (MQ=255)
                                                               tCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTCGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCGGGTgaagaa                                                                                                     <  2:681736/103‑1 (MQ=255)
                                                               tCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTCGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCGGGTgaagaa                                                                                                     >  1:681736/1‑103 (MQ=255)
                                                                  aaCGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTaaa                                                              <  1:15654/139‑1 (MQ=255)
                                                                      gCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGa                                                          >  2:1120370/1‑139 (MQ=255)
                                                                               gtGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                        <  1:797020/116‑1 (MQ=255)
                                                                               gtGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                        >  2:797020/1‑116 (MQ=255)
                                                                               gtGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTc                                                 >  1:1363877/1‑139 (MQ=255)
                                                                                tGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     >  2:321789/1‑86 (MQ=255)
                                                                                tGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     <  1:321789/86‑1 (MQ=255)
                                                                                 gACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTccc                                               >  1:349117/1‑139 (MQ=255)
                                                                                    cAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTg                                            >  2:457680/1‑139 (MQ=255)
                                                                                        ttCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCg                                        <  1:457680/139‑1 (MQ=255)
                                                                                            cGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAAc                                                                         <  1:282931/102‑1 (MQ=255)
                                                                                            cGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAAc                                                                         >  2:282931/1‑102 (MQ=255)
                                                                                                 cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGgcgc                                                                                                                          >  2:1412980/1‑48 (MQ=255)
                                                                                                 cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGgcgc                                                                                                                          <  1:1412980/48‑1 (MQ=255)
                                                                                                  gTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAg                              >  1:857160/1‑139 (MQ=255)
                                                                                                     gACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTAt                           >  2:676791/1‑139 (MQ=255)
                                                                                                      aCAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGAt                                                                                                                                >  2:683719/1‑37 (MQ=38)
                                                                                                      aCAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGAt                                                                                                                                <  1:683719/37‑1 (MQ=38)
                                                                                                          tACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCgg                      <  1:1165516/139‑1 (MQ=255)
                                                                                                           aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                                         <  1:1183359/103‑1 (MQ=255)
                                                                                                           aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                                         >  2:1183359/1‑103 (MQ=255)
                                                                                                             cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCaa                   >  2:213089/1‑139 (MQ=255)
                                                                                                             cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCaa                   >  2:1195011/1‑139 (MQ=255)
                                                                                                                cGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCggg                                                                                  >  1:8583/1‑73 (MQ=255)
                                                                                                                cGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCggg                                                                                  <  2:8583/73‑1 (MQ=255)
                                                                                                                 gggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                      <  1:698995/116‑1 (MQ=255)
                                                                                                                 gggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                      >  2:698995/1‑116 (MQ=255)
                                                                                                                  ggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                         >  1:1019138/1‑112 (MQ=255)
                                                                                                                  ggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                         <  2:1019138/112‑1 (MQ=255)
                                                                                                                   gTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCAc             <  2:857160/139‑1 (MQ=255)
                                                                                                                      cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     >  2:1472335/1‑48 (MQ=255)
                                                                                                                      cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     >  2:638424/1‑48 (MQ=255)
                                                                                                                      cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     <  1:638424/48‑1 (MQ=255)
                                                                                                                      cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     <  1:1472335/48‑1 (MQ=255)
                                                                                                                      cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTa                                                                <  2:821645/85‑1 (MQ=255)
                                                                                                                      cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTa                                                                >  1:821645/1‑85 (MQ=255)
                                                                                                                      cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACtga          >  1:1032036/1‑139 (MQ=255)
                                                                                                                         gtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     >  2:1320904/1‑45 (MQ=255)
                                                                                                                         gtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     <  1:1320904/45‑1 (MQ=255)
                                                                                                                         gtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACtgaatg       <  2:788924/139‑1 (MQ=255)
                                                                                                                             gtaaGTTTGTTAATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCTCCAGACCGTTCGGGGTATCAAACCTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGAGGCAAGGCCACTGAATGAAGc   >  2:875949/4‑139 (MQ=255)
                                                                                                                              tGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGcc  >  2:76406/1‑139 (MQ=255)
                                                                                                                                  tttGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     <  2:171372/36‑1 (MQ=255)
                                                                                                                                  tttGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                                     >  1:171372/1‑36 (MQ=255)
                                                                                                                                       |                                                                                                                                 
TATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCC  >  NC_000913/4297533‑4297797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑