Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,634	C→T	A378V (GCG→GTG)	proP →	proline/glycine betaine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,634	0	C	T	100.0%	130.9 / NA	41	A378V (GCG→GTG)	proP	proline/glycine betaine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

CCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTA  >  NC_000913/4331502‑4331766                                                                                                                                     |                                                                                                                                     cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGtt                                                                                                                                >  1:446851/1‑139 (MQ=255)      tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                           <  2:446851/139‑1 (MQ=255)      tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                           >  2:898423/1‑139 (MQ=255)      tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                           >  2:47735/1‑139 (MQ=255)                                   gATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCTCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                              <  2:895943/139‑1 (MQ=255)                                    aTTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcgg                                                                                             >  1:713276/1‑139 (MQ=255)                                     ttAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTTCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcggc                                                                                            <  1:71378/139‑1 (MQ=255)                                                          tGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGt                                                                       >  2:406956/1‑139 (MQ=255)                                                                                gCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                        <  1:107836/68‑1 (MQ=255)                                                                                gCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                        >  2:107836/1‑68 (MQ=255)                                                                                gCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTg                                                 <  2:713276/139‑1 (MQ=255)                                                                                 cTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTgg                                                >  1:442182/1‑139 (MQ=255)                                                                                  tGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTggc                                               >  2:960002/1‑139 (MQ=255)                                                                                   ggCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                        <  2:76704/65‑1 (MQ=255)                                                                                   ggCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                        >  1:76704/1‑65 (MQ=255)                                                                                    gCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTggcgg                                             >  2:367554/1‑139 (MQ=255)                                                                                                    gCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                      >  2:914438/1‑50 (MQ=255)                                                                                                    gCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                      <  1:914438/50‑1 (MQ=255)                                                                                                     cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                              <  2:589555/73‑1 (MQ=255)                                                                                                     cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                              >  2:965190/1‑73 (MQ=255)                                                                                                     cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                              <  1:965190/73‑1 (MQ=255)                                                                                                     cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                              >  1:589555/1‑73 (MQ=255)                                                                                                      tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                           >  2:1018896/1‑139 (MQ=255)                                                                                                          cGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                <  2:508229/66‑1 (MQ=255)                                                                                                          cGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                >  1:508229/1‑66 (MQ=255)                                                                                                                ttATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggc                                                                                               <  1:887294/61‑1 (MQ=255)                                                                                                                ttATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggc                                                                                               >  2:887294/1‑61 (MQ=255)                                                                                                                   tGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTTGAAAGCTCGCAGAATCtgat              <  2:442182/139‑1 (MQ=255)                                                                                                                   tGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtgat              <  1:960002/139‑1 (MQ=255)                                                                                                                       ttCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcggc                                                                                            <  2:1213790/57‑1 (MQ=255)                                                                                                                       ttCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcggc                                                                                            >  1:1213790/1‑57 (MQ=255)                                                                                                                        tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAttt                                                                                 <  2:358296/67‑1 (MQ=255)                                                                                                                        tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAttt                                                                                 >  1:358296/1‑67 (MQ=255)                                                                                                                        tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc         <  1:367554/139‑1 (MQ=255)                                                                                                                         cTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc        <  1:1018896/139‑1 (MQ=255)                                                                                                                          tACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTg                                                 <  2:288574/97‑1 (MQ=255)                                                                                                                          tACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTg                                                 >  1:288574/1‑97 (MQ=255)                                                                                                                             cTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcctgcc    <  1:898423/139‑1 (MQ=255)                                                                                                                               tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                              >  1:703013/1‑47 (MQ=255)                                                                                                                               tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                              <  2:703013/47‑1 (MQ=255)                                                                                                                               tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTa  >  1:424565/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                     CCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTA  >  NC_000913/4331502‑4331766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑