| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,395,058 | C→T | G331G (GGC→GGT) | nnr → | bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,395,058 | 0 | C | T | 97.9% | 147.4 / NA | 48 | G331G (GGC→GGT) | nnr | bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/26); minor base G (1/0); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACG > NC_000913/4394929‑4395184 | gACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAagag > 1:1335451/1‑139 (MQ=255) ccGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTgg < 2:1335451/139‑1 (MQ=255) gTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGaa < 2:337472/139‑1 (MQ=255) gcgcCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGc > 2:1015362/1‑139 (MQ=255) ccgcTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCaaa < 2:942207/139‑1 (MQ=255) tGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAAt < 2:311516/139‑1 (MQ=255) aCGACCGGAATTGATGGTGCATGAAATGAAGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGGGGGGATTGGTCCCGGTCTGGGGCAGCAAGAGGGGGGGGAAAAAGGACTGCAAAAAGTTGAGAAtttt > 2:471320/1‑139 (MQ=255) gACCGGAATTGATGGTGCATGAACTGACGCTGGACTCTCTTACCGGAAGCCTCGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCaa > 2:871088/1‑98 (MQ=255) gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCaa < 1:871088/98‑1 (MQ=255) gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg < 1:273685/139‑1 (MQ=255) gAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCa > 2:930815/1‑89 (MQ=255) aTTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg < 1:1094740/139‑1 (MQ=255) gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtg < 1:1015362/139‑1 (MQ=255) aTGGTGGATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGt < 2:235445/91‑1 (MQ=255) aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGt > 1:235445/1‑91 (MQ=255) tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgtt > 1:808732/1‑139 (MQ=255) gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg > 1:1362735/1‑130 (MQ=255) gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg < 2:1362735/130‑1 (MQ=255) gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGAGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg > 2:791825/1‑139 (MQ=255) tGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa < 2:808732/139‑1 (MQ=255) aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg > 1:288150/1‑136 (MQ=255) aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg < 2:288150/136‑1 (MQ=255) ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc > 1:129848/1‑113 (MQ=255) ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc < 2:129848/113‑1 (MQ=255) ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTg < 1:1504689/139‑1 (MQ=255) aCTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGaa > 1:1466148/1‑139 (MQ=255) aCTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGaa > 2:726501/1‑139 (MQ=255) ctctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAAc > 2:939996/1‑139 (MQ=255) ctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc < 1:471320/139‑1 (MQ=255) aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat < 2:1299015/139‑1 (MQ=255) gAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg > 1:873911/1‑102 (MQ=255) gAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg < 2:873911/102‑1 (MQ=255) ggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAg > 2:92721/1‑139 (MQ=255) aTGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTca < 1:452021/139‑1 (MQ=255) tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg < 1:1422932/98‑1 (MQ=255) tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg > 2:1422932/1‑98 (MQ=255) tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg < 2:1047151/115‑1 (MQ=255) tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg > 1:1047151/1‑115 (MQ=255) gtggtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcaca < 2:1466148/139‑1 (MQ=255) ggtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGTGAGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAAt < 1:791825/139‑1 (MQ=255) agagacagggTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATcg < 1:817742/131‑1 (MQ=255) ggtgATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATcgc < 1:939996/139‑1 (MQ=255) gATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTg > 2:1383024/1‑139 (MQ=255) aTTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaaa < 2:386612/69‑1 (MQ=255) aTTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaaa > 1:386612/1‑69 (MQ=255) tCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCCATCCCGATa < 1:1014304/116‑1 (MQ=255) tCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATa > 2:1014304/1‑116 (MQ=255) tCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg > 1:1430850/1‑139 (MQ=255) | GACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACG > NC_000913/4394929‑4395184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |