Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,146	C→T	W104* (TGG→TGA)	fecE ←	ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,146	0	C	T	100.0%	197.2 / NA	62	W104* (TGG→TGA)	fecE	ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/31);  total (31/31)

CCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAA  >  NC_000913/4511010‑4511280                                                                                                                                         |                                                                                                                                       ccAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCa                                                                                                                                      <  2:933579/139‑1 (MQ=255) ccAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCa                                                                                                                                      <  2:603466/139‑1 (MQ=255)     aaaTGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACCCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGa                                                                                                                                  >  1:299384/1‑139 (MQ=255)                              gAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCA‑CTCCTg                                                                                                        >  1:1184771/1‑139 (MQ=255)                                   cTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACt                                                                                                    >  2:743197/1‑139 (MQ=255)                                        ttAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                               <  1:1228675/139‑1 (MQ=255)                                                        aaGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTaaa                                                                               <  1:743197/139‑1 (MQ=255)                                                         aGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAAt                                                                              <  2:387574/139‑1 (MQ=255)                                                                       ggTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAg                                                                                                              >  2:589543/1‑93 (MQ=255)                                                                       ggTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAg                                                                                                              <  1:589543/93‑1 (MQ=255)                                                                             gTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaa                                                          <  1:1490176/139‑1 (MQ=255)                                                                              ttCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGaaa                                                         >  2:349191/1‑139 (MQ=255)                                                                                   ggCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                               <  2:388111/96‑1 (MQ=255)                                                                                   ggCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGAt                                                                                               >  1:388111/1‑96 (MQ=255)                                                                                     cGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCg                                                  >  1:669794/1‑139 (MQ=255)                                                                                       aCATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcat                                                                 >  1:337773/1‑121 (MQ=255)                                                                                       aCATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                                 <  2:337773/122‑1 (MQ=255)                                                                                        cATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGc                                               >  1:401108/1‑139 (MQ=255)                                                                                         aTTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGcc                                              <  1:252309/139‑1 (MQ=255)                                                                                                  tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          >  2:1417173/1‑86 (MQ=255)                                                                                                  tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          <  2:1145085/86‑1 (MQ=255)                                                                                                  tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          <  1:1417173/86‑1 (MQ=255)                                                                                                  tGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          >  1:1145085/1‑86 (MQ=255)                                                                                                     aTTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCA‑CTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATg                                 <  2:1184771/139‑1 (MQ=255)                                                                                                       tGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                >  1:540486/1‑139 (MQ=255)                                                                                                        gTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATgag                               <  1:349191/139‑1 (MQ=255)                                                                                                            tCAGCGGAGAGACGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa                           <  2:401108/139‑1 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                                >  2:574057/1‑47 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAAcc                                                                                                                <  1:574057/47‑1 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGc                                                                         <  2:932758/86‑1 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGc                                                                         >  1:932758/1‑86 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGa                                                           <  1:642406/100‑1 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGa                                                           >  2:642406/1‑100 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCaa                                            >  1:532210/1‑115 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCaa                                            <  2:532210/115‑1 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGAt                                  >  2:916565/1‑125 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt                    >  1:596133/1‑139 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt                    >  2:1232299/1‑139 (MQ=255)                                                                                                                   agagaCGCCCCCAGAGTGACAGTCACGGATTACGACCATACGAAACCAGCTCATGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGTCCAACTGGCGCGAt                                  <  1:916565/125‑1 (MQ=255)                                                                                                                    gagaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATg                   <  2:540486/139‑1 (MQ=255)                                                                                                                     agaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                >  2:612931/1‑125 (MQ=255)                                                                                                                     agaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                <  1:467643/125‑1 (MQ=255)                                                                                                                     agaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                >  2:467643/1‑125 (MQ=255)                                                                                                                     agaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATga                                <  1:612931/125‑1 (MQ=255)                                                                                                                     agaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            <  2:1261523/129‑1 (MQ=255)                                                                                                                     agaCGCCCCCAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGc                            >  1:1261523/1‑129 (MQ=255)                                                                                                                             ccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCgg                                                 >  1:1047483/1‑100 (MQ=255)                                                                                                                             ccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCgg                                                 <  2:1047483/100‑1 (MQ=255)                                                                                                                             ccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt                    >  2:1252868/1‑129 (MQ=255)                                                                                                                             ccAGAGTGACAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTAt                    <  1:1252868/129‑1 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          >  1:855332/1‑51 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCct                                                                                          <  2:855332/51‑1 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                                 <  2:1001830/76‑1 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGgcag                                                                 >  1:1001830/1‑76 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACt                                          <  1:780608/99‑1 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACt                                          >  2:780608/1‑99 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa                           >  2:472992/1‑114 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCa                           <  1:472992/114‑1 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCgaga    >  2:1424660/1‑137 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCgaga    <  1:1424660/137‑1 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGaaa  >  1:91765/1‑139 (MQ=255)                                                                                                                                     aCAGTCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGaaa  >  1:1369214/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       CCAGAAATGCGCGCTGGCGCTGACCGCCGGAAAGCTCGGTTAACCGACGAACGGCAAGATGATTGATCCGGGTCTGGTTCATGGCGACATTAACTCGTGCATTGTCTTCAGCGGAGAGACGCCCCCAGAGTGACAGCCAGGGATTACGACCATACGAAACCAGCTCCTGGACTGTGATCCCCTCTGGCGTTAAATGGTGCTGAGGCAGCAGCGAAAGCCTGCGGGCCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAA  >  NC_000913/4511010‑4511280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑