| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 468,510 | A→G | E43G (GAG→GGG) | ybaO → | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 468,510 | 0 | A | G | 97.8% | 153.4 / ‑4.6 | 45 | E43G (GAG→GGG) | ybaO | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (25/19); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
GGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACG > NC_000913/468379‑468637 | ggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga < 2:475467/135‑1 (MQ=255)ggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga > 1:475467/1‑135 (MQ=255) cTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg > 1:116479/1‑139 (MQ=255) aTGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCC‑TGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAt > 2:556887/1‑139 (MQ=255) aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga > 2:519498/1‑115 (MQ=255) aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga < 1:519498/115‑1 (MQ=255) aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGt > 2:1473677/1‑139 (MQ=255) gCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATc > 2:271756/1‑139 (MQ=255) tACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGa < 1:1473677/139‑1 (MQ=255) agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATc > 1:1474793/1‑129 (MQ=255) agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATc < 2:1474793/129‑1 (MQ=255) gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg < 2:682189/112‑1 (MQ=255) gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg > 1:682189/1‑112 (MQ=255) ttGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTc > 2:378951/1‑139 (MQ=255) cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa > 2:803942/1‑89 (MQ=255) cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa < 1:803942/89‑1 (MQ=255) cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCt < 2:116479/139‑1 (MQ=255) ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg < 1:221724/92‑1 (MQ=255) ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg > 2:221724/1‑92 (MQ=255) ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGcc < 1:937465/94‑1 (MQ=255) ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGcc > 2:937465/1‑94 (MQ=255) gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATaa > 2:162086/1‑139 (MQ=255) aGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACg < 1:162086/139‑1 (MQ=255) ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg < 2:1084421/55‑1 (MQ=255) ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg > 1:1084421/1‑55 (MQ=255) ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCgg > 2:1247499/1‑67 (MQ=255) ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCgg < 1:1247499/67‑1 (MQ=255) ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg < 2:1042619/75‑1 (MQ=255) ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg > 1:1042619/1‑75 (MQ=255) ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg < 1:616729/39‑1 (MQ=255) ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg > 2:616729/1‑39 (MQ=255) ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATg > 1:726138/1‑139 (MQ=255) ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATg > 1:384597/1‑139 (MQ=255) ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATg > 1:451745/1‑139 (MQ=255) cccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATgg > 2:1290003/1‑139 (MQ=255) cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTa > 1:602967/1‑139 (MQ=255) tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTAtt > 1:590395/1‑139 (MQ=255) gCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTg > 2:776622/1‑139 (MQ=255) gAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCtt > 1:590366/1‑37 (MQ=255) gAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCtt < 2:590366/37‑1 (MQ=255) aGCGCCTGAAACGGCTGGGGGACGACGGTATCCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCt < 1:556887/139‑1 (MQ=255) ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc < 1:1467739/81‑1 (MQ=255) ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc > 2:1467739/1‑81 (MQ=255) ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCaa < 2:97317/106‑1 (MQ=255) ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCaa > 1:97317/1‑106 (MQ=255) cTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt > 2:18747/1‑92 (MQ=255) cTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt < 1:18747/92‑1 (MQ=255) gAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACg < 2:451745/139‑1 (MQ=255) gAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACg < 1:378951/139‑1 (MQ=255) | GGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACG > NC_000913/468379‑468637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |