Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	478,533	C→T	A29T (GCC→ACC)	ylaB ←	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	478,533	0	C	T	100.0%	282.2 / NA	87	A29T (GCC→ACC)	ylaB	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/41);  total (46/41)

AGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGT  >  NC_000913/478402‑478663                                                                                                                                    |                                                                                                                                   aGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGTCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             >  1:378757/1‑139 (MQ=255)             tCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCg                                                                                                                 >  1:1110816/1‑139 (MQ=255)                ttCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGTCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                              <  2:378757/139‑1 (MQ=255)                  ccAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                            <  2:1110816/139‑1 (MQ=255)                    aGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgca                                                                                                          >  2:112655/1‑139 (MQ=255)                       tCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcagca                                                                                                       >  1:1303766/1‑139 (MQ=255)                          tGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGGGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATa                                                                                                    >  1:880867/1‑139 (MQ=255)                                gcATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcca                                                                                                                              <  2:855537/107‑1 (MQ=255)                                gcATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcca                                                                                                                              >  1:855537/1‑107 (MQ=255)                                gcATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaaa                                                                                              >  1:98182/1‑139 (MQ=255)                                   tCTTTCCCTTGTGTCACCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGaa                                                                                           >  1:834939/1‑139 (MQ=255)                                    cTTTCCCTTGTGTCGCCAGTTGATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAGACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAAt                                                                                          <  2:880867/139‑1 (MQ=255)                                             gtgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                                 <  2:341970/139‑1 (MQ=255)                                              tgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                              <  2:769889/109‑1 (MQ=255)                                              tgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                              >  1:769889/1‑109 (MQ=255)                                              tgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTccc                                                                                >  1:1020069/1‑139 (MQ=255)                                                tCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCGATAAACGATGTTTCTACCTTCTGATGGGTCAGCCAGATGCTTAAGCCCACAgg                                                                                                            >  2:997866/1‑109 (MQ=255)                                                tCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGAc                                                                                                               >  2:412029/1‑106 (MQ=255)                                                tCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGAc                                                                                                               <  1:412029/106‑1 (MQ=255)                                                tCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                            <  1:997866/109‑1 (MQ=255)                                                tCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcagc                                                                                                        <  1:467085/113‑1 (MQ=255)                                                tCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcagc                                                                                                        >  2:467085/1‑113 (MQ=255)                                                   ccACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAAt                                                                           >  2:140154/1‑139 (MQ=255)                                                        ttATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACt                                                                                                  >  2:543778/1‑111 (MQ=255)                                                        ttATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACt                                                                                                  <  1:543778/111‑1 (MQ=255)                                                          aTTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCg                                                                    <  1:112655/139‑1 (MQ=255)                                                             ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGAcc                                                                 >  1:1301024/1‑139 (MQ=255)                                                               cTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                               <  1:140154/139‑1 (MQ=255)                                                                 cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             <  2:42012/75‑1 (MQ=255)                                                                 cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             >  1:42012/1‑75 (MQ=255)                                                                 cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGAt                                                             >  2:410794/1‑139 (MQ=255)                                                                  gAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcca                                                                                                                              >  2:1146567/1‑73 (MQ=255)                                                                  gAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcca                                                                                                                              <  1:1146567/73‑1 (MQ=255)                                                                   aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGt                                                           >  1:1453759/1‑139 (MQ=255)                                                                   aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGt                                                           >  1:1359818/1‑139 (MQ=255)                                                                   aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTACCGAAATAAGGCCGACCAGATGt                                                           >  1:793604/1‑139 (MQ=255)                                                                       gCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtg                                                       <  1:494210/139‑1 (MQ=255)                                                                        cGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGCTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     <  1:1020480/108‑1 (MQ=255)                                                                        cGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     >  2:1020480/1‑108 (MQ=255)                                                                        cGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtgt                                                      >  2:47137/1‑139 (MQ=255)                                                                        cGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtgt                                                      >  2:283820/1‑139 (MQ=255)                                                                             cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         >  2:418076/1‑83 (MQ=255)                                                                             cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         <  1:418076/83‑1 (MQ=255)                                                                             cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                 >  1:406798/1‑139 (MQ=255)                                                                             cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                 <  2:1020069/139‑1 (MQ=255)                                                                                   gaATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa                                                                                               <  2:1335326/87‑1 (MQ=255)                                                                                   gaATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa                                                                                               >  1:1335326/1‑87 (MQ=255)                                                                                       aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       <  2:1453759/139‑1 (MQ=255)                                                                                         gTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCt                                     <  2:1359818/139‑1 (MQ=255)                                                                                            tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAATCCGACAGGCAGCAATACTGAAAGAATCAg                                                                                       >  1:785218/1‑86 (MQ=255)                                                                                            tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAATCCGACAGGCAGCAATACTGAAAGAATCAg                                                                                       <  2:785218/86‑1 (MQ=255)                                                                                            tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                            <  2:148441/65‑1 (MQ=255)                                                                                            tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                            >  1:148441/1‑65 (MQ=255)                                                                                            tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttat                                  >  2:384768/1‑139 (MQ=255)                                                                                             ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       >  2:534494/1‑133 (MQ=255)                                                                                             ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       <  1:534494/133‑1 (MQ=255)                                                                                              cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAg                                                                                       <  2:324026/84‑1 (MQ=255)                                                                                              cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAg                                                                                       >  1:324026/1‑84 (MQ=255)                                                                                              cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttatta                                >  1:486464/1‑139 (MQ=255)                                                                                                gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAac                              <  1:384768/139‑1 (MQ=255)                                                                                                 ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:410794/139‑1 (MQ=255)                                                                                                 ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  2:834939/139‑1 (MQ=255)                                                                                                 ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:283820/139‑1 (MQ=255)                                                                                                 ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:1447619/139‑1 (MQ=255)                                                                                                  tctTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             <  2:661000/42‑1 (MQ=255)                                                                                                  tctTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             >  1:661000/1‑42 (MQ=255)                                                                                                  tctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAacac                            <  2:406798/139‑1 (MQ=255)                                                                                                   ctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttat                                  <  2:974122/132‑1 (MQ=255)                                                                                                   ctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttat                                  >  1:974122/1‑132 (MQ=255)                                                                                                   ctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  2:698980/137‑1 (MQ=255)                                                                                                   ctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             >  1:698980/1‑137 (MQ=255)                                                                                                    ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                            >  1:288014/1‑57 (MQ=255)                                                                                                    ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                            <  2:288014/57‑1 (MQ=255)                                                                                                      cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAac                              >  1:1059085/1‑133 (MQ=255)                                                                                                      cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAac                              <  2:1059085/133‑1 (MQ=255)                                                                                                      cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAgtg                        >  2:188418/1‑139 (MQ=255)                                                                                                            aCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                  <  2:793604/139‑1 (MQ=255)                                                                                                              gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGaaa                >  2:111993/1‑139 (MQ=255)                                                                                                               aTGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         >  1:1458384/1‑49 (MQ=255)                                                                                                               aTGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         <  2:1458384/49‑1 (MQ=255)                                                                                                                tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCg                                                                                                                 >  2:1034706/1‑40 (MQ=255)                                                                                                                tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCg                                                                                                                 <  1:1034706/40‑1 (MQ=255)                                                                                                                tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAg                                                                                       >  2:132443/1‑66 (MQ=255)                                                                                                                tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAg                                                                                       <  1:132443/66‑1 (MQ=255)                                                                                                                tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       <  1:792995/114‑1 (MQ=255)                                                                                                                tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       >  2:792995/1‑114 (MQ=255)                                                                                                                tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGt              >  2:671059/1‑139 (MQ=255)                                                                                                                          tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     >  1:1259452/1‑58 (MQ=255)                                                                                                                          tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     <  2:1259452/58‑1 (MQ=255)                                                                                                                           gctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCata                                         >  1:432717/1‑101 (MQ=255)                                                                                                                           gctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCata                                         <  2:432717/101‑1 (MQ=255)                                                                                                                            ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAAt                                                                                          >  1:105638/1‑51 (MQ=255)                                                                                                                            ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAAt                                                                                          <  2:105638/51‑1 (MQ=255)                                                                                                                            ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAacac                            <  1:876360/113‑1 (MQ=255)                                                                                                                            ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAacac                            >  2:876360/1‑113 (MQ=255)                                                                                                                            ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATtgt  >  1:616053/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                   AGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGT  >  NC_000913/478402‑478663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑