Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,696,452 (A)5→4 intergenic (‑381/+10) uidA ← / ← uidR beta‑D‑glucuronidase/transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,696,4480A.100.0% 262.5 / NA 63intergenic (‑377/+14)uidA/uidRbeta‑D‑glucuronidase/transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (31/32);  total (31/32)

ATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696313‑1696580
                                                                                                                                       |                                                                                                                                    
aTTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTGTCACCAGCCCGCCGaaaa                                                                                                                                   >  2:16135/1‑139 (MQ=255)
    ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTGTCACCAGCCCGCCGaaaagaga                                                                                                                               <  1:16135/139‑5 (MQ=255)
    ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  1:1079355/139‑5 (MQ=255)
    ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  1:631785/139‑5 (MQ=255)
         gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGaa                                                                                                                         >  1:451008/1‑139 (MQ=255)
          aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        >  2:1068245/1‑139 (MQ=255)
          aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        <  2:659362/139‑1 (MQ=255)
                tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  2:433588/139‑1 (MQ=255)
                  tcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGc                                                                                                                <  1:1068245/139‑1 (MQ=255)
                   cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCg                                                                                                               >  2:717306/1‑139 (MQ=255)
                   cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCg                                                                                                               >  1:794942/1‑139 (MQ=255)
                   cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCg                                                                                                               >  1:632620/1‑139 (MQ=255)
                     tGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGt                                                                                                             <  1:333732/139‑1 (MQ=255)
                          tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGa                                                                                                        >  1:1293902/1‑139 (MQ=255)
                                   aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                               <  1:717306/139‑1 (MQ=255)
                                   aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                               >  2:556373/1‑139 (MQ=255)
                                    gTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAat                                                                                              <  2:451008/139‑1 (MQ=255)
                                     tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               >  1:402756/1‑102 (MQ=255)
                                     tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:402756/106‑5 (MQ=255)
                                     tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:782491/106‑5 (MQ=255)
                                     tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               >  1:782491/1‑102 (MQ=255)
                                       aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        >  2:59952/1‑110 (MQ=255)
                                       aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAAt                                                                                                                        <  1:59952/110‑1 (MQ=255)
                                             ttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcgcgcg                                                                                     >  2:667538/1‑139 (MQ=255)
                                             ttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcgcgcg                                                                                     >  1:994/1‑139 (MQ=255)
                                               ggggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:384608/96‑5 (MQ=255)
                                               ggggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               >  1:384608/1‑92 (MQ=255)
                                               ggggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    <  2:175264/138‑1 (MQ=255)
                                               ggggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    >  1:175264/1‑138 (MQ=255)
                                                   ggAATTCTTCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCATCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATcc                                                                               <  1:781424/139‑1 (MQ=255)
                                                      aTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTg                                                                            <  2:794942/139‑1 (MQ=255)
                                                        tctcCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAg                                                                          <  2:1149505/139‑1 (MQ=255)
                                                                    ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGc                                                                                                                <  2:46879/89‑1 (MQ=255)
                                                                    ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGc                                                                                                                >  1:46879/1‑89 (MQ=255)
                                                                    ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                              >  1:1039417/1‑139 (MQ=255)
                                                                            aaTTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                      <  1:531555/139‑1 (MQ=255)
                                                                              tttttGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAg                                                    <  2:106534/139‑1 (MQ=255)
                                                                                    cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                              <  2:1293902/139‑1 (MQ=255)
                                                                                         agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    >  1:38449/1‑96 (MQ=255)
                                                                                         agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    <  2:38449/96‑1 (MQ=255)
                                                                                         agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCtt                                         >  1:1222274/1‑139 (MQ=255)
                                                                                                   atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  >  1:123623/1‑56 (MQ=255)
                                                                                                   atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  2:123623/56‑1 (MQ=255)
                                                                                                   atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg                                                                 >  1:261062/1‑105 (MQ=255)
                                                                                                   atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg                                                                 <  2:261062/105‑1 (MQ=255)
                                                                                                   atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  2:116532/134‑1 (MQ=255)
                                                                                                   atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    >  1:116532/1‑134 (MQ=255)
                                                                                                     atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  >  2:94886/1‑54 (MQ=255)
                                                                                                     atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  1:94886/54‑1 (MQ=255)
                                                                                                       gAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      >  1:1105439/1‑64 (MQ=255)
                                                                                                       gAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      <  2:1105439/64‑1 (MQ=255)
                                                                                                            gcgGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATGGCCAGTACACCg                     <  2:1039417/139‑1 (MQ=255)
                                                                                                                   caGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTc               <  1:556373/139‑1 (MQ=255)
                                                                                                                      ttATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGa            <  2:994/139‑1 (MQ=255)
                                                                                                                           aCCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:1312499/115‑1 (MQ=255)
                                                                                                                           aCCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               >  1:1312499/1‑115 (MQ=255)
                                                                                                                              aGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      >  1:818712/1‑41 (MQ=38)
                                                                                                                              aGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      <  2:818712/41‑1 (MQ=38)
                                                                                                                              aGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag    >  1:855917/1‑139 (MQ=255)
                                                                                                                               gCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                            >  1:1184792/1‑82 (MQ=255)
                                                                                                                               gCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                            <  2:1184792/82‑1 (MQ=255)
                                                                                                                               gCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGt   >  1:507562/1‑139 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                               >  1:47285/1‑46 (MQ=39)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                               <  2:47285/46‑1 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    <  2:591660/57‑1 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                    >  1:591660/1‑57 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                            <  1:472229/81‑1 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                            >  2:472229/1‑81 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    >  1:133325/1‑105 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  2:133325/105‑1 (MQ=255)
                                                                                                                                cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:196121/1‑139 (MQ=255)
                                                                                                                                  cgccgAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    >  2:425830/1‑119 (MQ=255)
                                                                                                                                  cgccgAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    <  1:425830/119‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
ATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696313‑1696580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: