Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,850,810 (C)5→4 intergenic (+117/‑50) sppA → / → ansA protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,8060C.100.0% 152.2 / NA 37intergenic (+113/‑54)sppA/ansAprotease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/17);  total (20/17)

TGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGG  >  NC_000913/1850676‑1850926
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tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCGCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 <  2:45016/139‑1 (MQ=255)
                 aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGc                                                                                                >  1:628878/1‑139 (MQ=255)
                          cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGat                                                                                       <  1:1170992/139‑1 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 >  1:1176236/1‑110 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 <  2:1176236/110‑1 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                    >  1:445221/1‑139 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                    >  1:1277780/1‑139 (MQ=255)
                               tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcat                                                                                  >  1:126043/1‑139 (MQ=255)
                                ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                 <  2:628878/139‑1 (MQ=255)
                                  ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                               <  1:1058923/139‑1 (MQ=255)
                                       aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 <  2:1275018/100‑1 (MQ=255)
                                       aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 >  1:1275018/1‑100 (MQ=255)
                                         aTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccct                                                                                                                      >  2:338189/1‑93 (MQ=255)
                                         aTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccct                                                                                                                      <  1:338189/94‑2 (MQ=255)
                                            gtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 <  2:313562/95‑1 (MQ=255)
                                            gtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 >  1:313562/1‑95 (MQ=255)
                                                gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGc                                                                 >  1:403280/1‑139 (MQ=255)
                                                       tattTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 >  2:400204/1‑84 (MQ=255)
                                                       tattTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 <  1:400204/84‑1 (MQ=255)
                                                               aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt                                                  >  2:872319/1‑139 (MQ=255)
                                                                  tCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                               <  2:403280/139‑1 (MQ=255)
                                                                  tCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                               <  2:126043/139‑1 (MQ=255)
                                                                   cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGt                                              >  1:254909/1‑139 (MQ=255)
                                                                     acgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc                                                                                                                       <  2:40653/65‑1 (MQ=255)
                                                                     acgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc                                                                                                                       >  1:40653/1‑65 (MQ=255)
                                                                                    gCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 <  1:762961/55‑1 (MQ=255)
                                                                                    gCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                 >  2:762961/1‑55 (MQ=255)
                                                                                     cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtca                                                                                   <  1:1037211/84‑1 (MQ=255)
                                                                                     cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtca                                                                                   >  2:1037211/1‑84 (MQ=255)
                                                                                       gTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtca                                                                                   <  2:1283478/82‑1 (MQ=255)
                                                                                       gTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtca                                                                                   >  1:1283478/1‑82 (MQ=255)
                                                                                        tttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCaaaa                                                             >  2:1040153/1‑103 (MQ=255)
                                                                                        tttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCaaaa                                                             <  1:1040153/103‑1 (MQ=255)
                                                                                                          tATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGa       >  1:628011/1‑139 (MQ=255)
                                                                                                            tCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc     >  1:938200/1‑139 (MQ=255)
                                                                                                             ccGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa    <  2:628011/139‑1 (MQ=255)
                                                                                                               gCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAgg  >  1:1105974/1‑139 (MQ=255)
                                                                                                                       tCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                 <  2:1288465/52‑1 (MQ=255)
                                                                                                                       tCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                 >  1:1288465/1‑52 (MQ=255)
                                                                                                                             gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa    <  2:167882/123‑1 (MQ=255)
                                                                                                                             gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa    >  1:167882/1‑123 (MQ=255)
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TGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGG  >  NC_000913/1850676‑1850926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: