Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,199,764 (T)5→6 coding (1043/1128 nt) intE ← e14 prophage, putative integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,199,7591.T98.2% 180.5 / ‑3.6 57coding (1048/1128 nt)intEe14 prophage, putative integrase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (24/32);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

TTAGTTTTTATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCAATGTAT  >  NC_000913/1199623‑1199896
                                                                                                                                         |                                                                                                                                         
ttAGTTTTTATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGtt                                                                                                                                         >  2:329385/1‑139 (MQ=255)
    tttttATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGtttttt                                                                                                                                     >  2:366034/1‑138 (MQ=255)
      tttATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGttttttgt                                                                                                                                   >  1:403285/1‑136 (MQ=255)
       ttATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGttttttgtg                                                                                                                                  <  1:167520/139‑5 (MQ=255)
               tgCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAAc                                                                                                                           <  1:219475/139‑1 (MQ=255)
                 cGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAAccc                                                                                                                         <  1:329385/139‑1 (MQ=255)
                   gTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  2:998165/139‑1 (MQ=255)
                   gTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  2:503900/139‑1 (MQ=255)
                   gTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  2:403285/139‑1 (MQ=255)
                   gTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:830609/139‑1 (MQ=255)
                     gagaAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGt                                                                                                                     >  1:982842/1‑139 (MQ=255)
                      agaAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  1:1210365/139‑1 (MQ=255)
                        aaaTTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCt                                                                                                                  <  1:793303/139‑1 (MQ=255)
                         aaTTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGttttttg                                                                                                                                    >  2:1257542/1‑117 (MQ=255)
                         aaTTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGttttttg                                                                                                                                    <  1:1257542/119‑3 (MQ=255)
                                tgtgGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAg                                                                                                          >  2:1231003/1‑139 (MQ=255)
                                 gtgGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGa                                                                                                         <  1:357192/139‑1 (MQ=255)
                                            aTGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATaa                                                                                              <  1:1225468/139‑1 (MQ=255)
                                               cTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGa                                                                                           >  2:503966/1‑139 (MQ=255)
                                                         cacaACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGtt                                                                                                     <  1:1263685/119‑1 (MQ=255)
                                                         cacaACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGtt                                                                                                     >  2:1263685/1‑119 (MQ=255)
                                                                cTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCtt                                                                                       <  1:323892/126‑1 (MQ=255)
                                                                cTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCtt                                                                                       >  2:323892/1‑126 (MQ=255)
                                                                   cTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCg                                                                       <  1:366034/139‑1 (MQ=255)
                                                                   cTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCg                                                                       <  1:1231003/139‑1 (MQ=255)
                                                                      tGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAgg                                                                   >  2:81485/1‑139 (MQ=255)
                                                                        cTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGt                                                                  <  1:355998/139‑1 (MQ=255)
                                                                          ccATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGa                                                                <  2:982842/139‑1 (MQ=255)
                                                                             tCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:215626/84‑1 (MQ=255)
                                                                             tCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    >  1:215626/1‑84 (MQ=255)
                                                                              ccTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAAccc                                                            <  1:503966/139‑1 (MQ=255)
                                                                                  tACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCtctc                                                        >  2:1062516/1‑139 (MQ=255)
                                                                                        gATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       >  2:891379/1‑70 (MQ=255)
                                                                                        gATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:891379/70‑1 (MQ=255)
                                                                                        gATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGa                                                                             >  1:317047/1‑112 (MQ=255)
                                                                                        gATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGa                                                                             <  2:317047/112‑1 (MQ=255)
                                                                                         atcatcATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCt                                                                                                                              >  2:305722/1‑62 (MQ=255)
                                                                                         atcatcATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCt                                                                                                                              <  1:305722/62‑1 (MQ=255)
                                                                                         atcatcATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACg                                                                                                       <  2:258746/85‑1 (MQ=255)
                                                                                         atcatcATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACg                                                                                                       >  1:258746/1‑85 (MQ=255)
                                                                                         atcatcATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTc                                                 >  2:1033596/1‑139 (MQ=255)
                                                                                             tcatGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAAtt                                             <  2:871177/139‑1 (MQ=255)
                                                                                                     aCGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTaa                                     >  1:127667/1‑139 (MQ=255)
                                                                                                      cGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       >  2:285631/1‑56 (MQ=255)
                                                                                                      cGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:285631/56‑1 (MQ=255)
                                                                                                      cGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTc                                                                                                    <  2:1276034/75‑1 (MQ=255)
                                                                                                      cGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTc                                                                                                    >  1:1276034/1‑75 (MQ=255)
                                                                                                        aTCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAAcc                                                             >  2:516583/1‑112 (MQ=255)
                                                                                                        aTCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAAcc                                                             <  1:516583/112‑1 (MQ=255)
                                                                                                         tCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAAcc                                                             >  1:59060/1‑111 (MQ=255)
                                                                                                         tCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAAcc                                                             <  2:59060/111‑1 (MQ=255)
                                                                                                           ggTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAAtctct                               >  2:1071430/1‑139 (MQ=255)
                                                                                                            gTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:745936/50‑1 (MQ=255)
                                                                                                            gTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       >  2:349460/1‑50 (MQ=255)
                                                                                                            gTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       >  2:745936/1‑50 (MQ=255)
                                                                                                            gTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:349460/50‑1 (MQ=255)
                                                                                                              ttgttgttgTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTg                                                                 <  2:460830/102‑1 (MQ=255)
                                                                                                              ttgttgttgTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTg                                                                 >  1:460830/1‑102 (MQ=255)
                                                                                                                gttgttgtGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCtt                          <  1:1062516/139‑1 (MQ=255)
                                                                                                                      gtgtTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTaa                    >  1:898498/1‑139 (MQ=255)
                                                                                                                         ttttATGACCAAGTAGTTTTTTGTGTGTCTGACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGTTCATGGAATGTTGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATGTTTCGCTTTATTAAAAt                 >  1:444862/1‑139 (MQ=255)
                                                                                                                                   aaGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTc         <  2:164211/137‑1 (MQ=255)
                                                                                                                                   aaGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTc         >  1:164211/1‑137 (MQ=255)
                                                                                                                                       agtTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCAATGTAt  <  1:81485/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TTAGTTTTTATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCAATGTAT  >  NC_000913/1199623‑1199896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: