| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,250,629 | (C)5→6 | coding (210/1071 nt) | dhaK ← | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,250,624 | 1 | . | C | 96.2% | 178.8 / ‑0.1 | 52 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (22/28); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
CTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑C‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG > NC_000913/1250493‑1250754 | cTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c‑cccccg < 1:370846/139‑3 (MQ=255) tCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGc < 1:448192/139‑1 (MQ=255) cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGatat > 2:843912/1‑139 (MQ=255) ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑aa > 1:897010/1‑139 (MQ=255) ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑aa > 1:473313/1‑139 (MQ=255) cGGTTTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AAc < 1:969456/139‑1 (MQ=255) cGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTg < 1:8556/128‑1 (MQ=255) cGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTg > 2:8556/1‑128 (MQ=255) gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGAc > 1:32925/1‑128 (MQ=255) gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGAc < 2:32925/128‑1 (MQ=255) tAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGa < 1:1290516/128‑1 (MQ=255) tAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGa > 2:1290516/1‑128 (MQ=255) gATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGa > 1:599467/1‑116 (MQ=255) gATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGa < 2:599467/116‑1 (MQ=255) gATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGAcc < 1:585288/118‑1 (MQ=255) gATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGAcc > 2:585288/1‑118 (MQ=255) gATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGAc < 1:719022/117‑1 (MQ=255) gATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGAc > 2:719022/1‑117 (MQ=255) gTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGc > 2:782771/1‑96 (MQ=255) gTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGc < 1:782771/96‑1 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 1:644498/139‑1 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 1:647621/139‑1 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTgccgc > 2:178776/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTgccgc > 2:584273/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTATCCGCTgccgc > 1:851264/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCG‑‑AT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 2:400709/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑C‑CCCCCAAAGGATTCCCTTGCCGATTTAAACCACA‑TTCCTCGGGCTGTTTCCGCTgccgcc > 1:73429/1‑139 (MQ=255) cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CACCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc > 2:939764/1‑139 (MQ=255) ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAccc > 2:988859/1‑139 (MQ=255) aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAcag < 1:648700/139‑1 (MQ=255) ttGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGc < 2:108209/139‑1 (MQ=255) gCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCG‑‑AT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACt < 1:400709/139‑1 (MQ=255) aTGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATc > 1:176095/1‑100 (MQ=255) aTGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATc < 2:176095/100‑1 (MQ=255) tGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtt < 1:594368/139‑1 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc < 2:1257404/139‑1 (MQ=255) gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc < 1:843912/139‑1 (MQ=255) cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCt > 1:615913/1‑98 (MQ=255) cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCt < 2:615913/98‑1 (MQ=255) aGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAg < 2:509725/139‑1 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCg < 2:149202/117‑1 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCg > 1:149202/1‑117 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt < 2:994559/139‑1 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 2:1225695/1‑139 (MQ=255) gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg < 2:622478/139‑1 (MQ=255) tCGGTGAGGTGAAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCggg < 1:584273/139‑1 (MQ=255) gaggtgaAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACACGACAGCAGGGCGATTTTTCTTGCAACAGGGGCATCAGCTCGGGTGACa < 2:851264/139‑1 (MQ=255) aggtgaAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACAt < 2:92682/139‑1 (MQ=255) ggtgaAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg > 2:221536/1‑131 (MQ=255) ggtgaAAATTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg < 1:221536/131‑1 (MQ=255) aaaaTTTCGCCCGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACAcc > 2:57158/1‑139 (MQ=255) ccGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGCGCATCGGCTCATGTCCGCTGCCGCCACCCGACAGCAGCGCGACTTTTCCTGCAACAGCGGCATCAGCTCGGGTGACATAAACCGGATCCTGac > 2:306649/1‑138 (MQ=255) cGGACAGGCC‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATg < 2:897010/139‑1 (MQ=255) | CTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑C‑CCCCGAAAGCATCCCCTGACCGATAT‑AACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG > NC_000913/1250493‑1250754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |