Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,399,985 (C)7→8 coding (252/516 nt) ogt ← O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,399,9781.C100.0% 127.5 / NA 36T87A (ACG→GCG) ogtO‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/19);  total (17/19)

GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAAT  >  NC_000913/1399841‑1400103
                                                                                                                                          |                                                                                                                             
gggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑c                                                                                                                              <  2:698015/139‑1 (MQ=255)
 ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc                                                                                                                             <  1:715621/139‑1 (MQ=255)
   aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc                                                                                                                           <  1:415517/139‑1 (MQ=255)
    atCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccc                                                                                                                          >  2:820595/2‑139 (MQ=255)
     tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc                                                                                                                         >  1:603086/1‑139 (MQ=255)
        aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccg                                                                                                                      <  2:1056745/139‑3 (MQ=255)
            gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                   <  1:859478/139‑1 (MQ=255)
            gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑c                                                                                                                              <  1:1114393/127‑1 (MQ=255)
            gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑c                                                                                                                              >  2:1114393/1‑127 (MQ=255)
              ttGCCGCA‑CAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGt                                                                                                                <  2:1014376/139‑1 (MQ=255)
              ctccagCACCAGCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAg                                                                                                                 >  1:1067574/6‑139 (MQ=255)
                   gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                            <  1:399988/139‑1 (MQ=255)
                   gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                            <  1:820595/139‑1 (MQ=255)
                        agcGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGt                                                                                                       <  2:1067574/137‑1 (MQ=255)
                             cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                  <  1:400365/139‑1 (MQ=255)
                             cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                  <  1:375038/139‑1 (MQ=255)
                             cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                  <  2:542484/139‑1 (MQ=255)
                             cACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                  <  1:565440/139‑1 (MQ=255)
                                                 cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCg                                                                                 >  1:499207/1‑136 (MQ=255)
                                                 cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCg                                                                                 <  2:499207/136‑1 (MQ=255)
                                                      ttGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAata                                                                         >  2:1028498/1‑139 (MQ=255)
                                                                  ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                             >  2:551612/1‑139 (MQ=255)
                                                                  ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                             <  1:224317/139‑1 (MQ=255)
                                                                       cGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGc                                                        <  1:1028498/139‑1 (MQ=255)
                                                                       cGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGc                                                        >  1:1043228/1‑139 (MQ=255)
                                                                                       cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccccccgtag                                                                                                                  >  1:386710/1‑58 (MQ=255)
                                                                                       cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccccccgtag                                                                                                                  <  2:386710/64‑7 (MQ=255)
                                                                                         cGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc                                                                                                                             <  2:1173236/51‑1 (MQ=255)
                                                                                         cGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc                                                                                                                             >  1:1173236/1‑51 (MQ=255)
                                                                                             ggggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                   <  1:339144/58‑1 (MQ=255)
                                                                                             ggggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                   >  2:339144/1‑58 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                   <  1:556166/47‑1 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGc                                                                                                                   >  2:556166/1‑47 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg                                                                                                             <  1:1019169/53‑1 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg                                                                                                             >  2:1019169/1‑53 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa                                                    >  1:545247/1‑110 (MQ=255)
                                                                                                        gTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa                                                    <  2:545247/110‑1 (MQ=255)
                                                                                                         tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTc                      >  2:756589/1‑139 (MQ=255)
                                                                                                               tttCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGATTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGcc                >  2:626880/1‑139 (MQ=255)
                                                                                                                 tCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCctt              >  1:822196/1‑139 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTAt                                                                                                     >  2:127220/1‑43 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTAt                                                                                                     <  1:127220/43‑1 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt     >  2:536665/1‑139 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt     >  1:1183591/1‑139 (MQ=255)
                                                                                                                          cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt     >  1:1131394/1‑139 (MQ=255)
                                                                                                                             gcTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGc                                               >  2:466301/1‑94 (MQ=255)
                                                                                                                             gcTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGc                                               <  1:466301/94‑1 (MQ=255)
                                                                                                                             gcTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAAt  <  1:536665/139‑1 (MQ=255)
                                                                                                                             gcTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAAt  <  2:967713/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                             
GGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAAT  >  NC_000913/1399841‑1400103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑