Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,421,650 (C)7→8 coding (532/858 nt) ydaU → Rac prophage, conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,421,6431.C91.8% 161.6 / 6.0 49coding (525/858 nt)ydaURac prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (25/20);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CAGATGTTCAAACAAAACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTCGAA  >  NC_000913/1421507‑1421770
                                                                                                                                         |                                                                                                                                
cAGATGTTCAAACAAAACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑cc                                                                                                                               <  2:774026/139‑1 (MQ=255)
  gATGTTCAAACAAAACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑cccc                                                                                                                             >  1:854596/1‑139 (MQ=255)
    tGTTCAAACAAAACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑cccccc                                                                                                                           <  1:528129/139‑1 (MQ=255)
        caaacaaaACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑ccccccccgg                                                                                                                       <  1:1206437/139‑4 (MQ=255)
        caaacaaaACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCcgg                                                                                                                       <  1:203101/139‑1 (MQ=255)
          aacaaaacaaGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCcgggg                                                                                                                     <  2:854596/139‑1 (MQ=255)
                acaaGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCg                                                                                                                <  1:1278493/139‑1 (MQ=255)
                                      gtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          <  2:924875/139‑1 (MQ=255)
                                      gtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          <  1:66199/139‑1 (MQ=255)
                                       ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAt                                                                                                                                   >  2:721613/1‑98 (MQ=255)
                                       ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAt                                                                                                                                   <  1:721613/98‑1 (MQ=255)
                                                      aaGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCg                                                                                                                >  2:62923/1‑101 (MQ=255)
                                                      aaGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCg                                                                                                                <  1:62923/101‑1 (MQ=255)
                                                          ggtgTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               <  1:1021876/114‑1 (MQ=255)
                                                          ggtgTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               >  2:1021876/1‑114 (MQ=255)
                                                          ggtgTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGaa                                                                      >  2:1077024/1‑139 (MQ=255)
                                                           gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               <  1:563024/113‑1 (MQ=255)
                                                           gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               >  2:563024/1‑113 (MQ=255)
                                                           gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               <  1:395324/113‑1 (MQ=255)
                                                           gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               >  2:395324/1‑113 (MQ=255)
                                                                      tgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          <  1:164208/107‑1 (MQ=255)
                                                                      tgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          >  2:164208/1‑107 (MQ=255)
                                                                      tgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAAACGAAGTGTCAAAAAGTTTGACCCTCTGGATATTACCTTGCCGTACCGGAGTTCTGt                                                          >  1:1044389/1‑139 (MQ=255)
                                                                            cAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAg                                                                                                              <  2:77173/80‑1 (MQ=255)
                                                                            cAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAg                                                                                                              >  1:77173/1‑80 (MQ=255)
                                                                                    aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTTTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCg                                              >  2:809429/1‑137 (MQ=255)
                                                                                    aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCg                                              <  1:809429/137‑1 (MQ=255)
                                                                                         aCTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAACCTCTGGATATTACTTTGCCGAACCGGATTTCTGTCTCGCTTTGGCggtggtgg                                       >  2:19425/1‑132 (MQ=255)
                                                                                              taaAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGa                                  >  1:546057/1‑139 (MQ=255)
                                                                                                      cAGGTAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt                                                    >  1:894291/1‑113 (MQ=255)
                                                                                                      cAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt                                                    <  2:894291/113‑1 (MQ=255)
                                                                                                       aGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCa                         >  1:979991/1‑139 (MQ=255)
                                                                                                       aGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCa                         >  1:1165038/1‑139 (MQ=255)
                                                                                                         aTAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                                                                         <  1:259118/73‑1 (MQ=255)
                                                                                                         aTAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                                                                         >  2:259118/1‑73 (MQ=255)
                                                                                                             aGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               <  1:505601/63‑1 (MQ=255)
                                                                                                             aGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                               >  2:505601/1‑63 (MQ=255)
                                                                                                                 cTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                                                                        >  1:757911/1‑66 (MQ=255)
                                                                                                                 cTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctct                                                                                        <  2:757911/66‑1 (MQ=255)
                                                                                                                   aaaaaCAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGa                                                                                     <  1:1093466/67‑1 (MQ=255)
                                                                                                                   aaaaaCAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGa                                                                                     >  2:1093466/1‑67 (MQ=255)
                                                                                                                   aaaaaCAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGt                                     <  2:587053/115‑1 (MQ=255)
                                                                                                                   aaaaaCAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGt                                     >  1:587053/1‑115 (MQ=255)
                                                                                                                          gATCCCCCCCTAAATCCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          >  2:1119031/1‑56 (MQ=255)
                                                                                                                          gATCCCCCCCTAAATCCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          <  1:1119031/56‑1 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          <  2:187789/54‑1 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                          >  1:187789/1‑54 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGc                                               <  2:1129593/97‑1 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGc                                               >  1:1129593/1‑97 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGAt       <  2:1263719/137‑1 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGAt       >  1:1263719/1‑137 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTc     >  1:964373/1‑139 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTc     >  2:1188228/1‑139 (MQ=255)
                                                                                                                           aTCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTc     >  1:66804/1‑139 (MQ=255)
                                                                                                                              ccccccTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTCGaa  <  1:1077024/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                
CAGATGTTCAAACAAAACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAAT‑‑CCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTCGAA  >  NC_000913/1421507‑1421770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: