Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,484,897 (C)6→7 coding (524/3594 nt) evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,484,8911.C97.3% 252.5 / ‑1.0 73coding (518/3594 nt)evgShybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (35/36);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC  >  NC_000913/2484754‑2485021
                                                                                                                                          |                                                                                                                                    
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                     <  1:1090917/139‑1 (MQ=255)
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                     >  2:285184/1‑139 (MQ=255)
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                     <  2:1301058/139‑1 (MQ=255)
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                     <  1:197725/139‑1 (MQ=255)
  cgccgcCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccc                                                                                                                                  >  2:1291803/1‑139 (MQ=255)
    ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                               >  2:584217/1‑139 (MQ=255)
         tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          <  2:787797/139‑5 (MQ=255)
         tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          <  1:584217/139‑5 (MQ=255)
          ctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccagac                                                                                                                         >  2:695817/1‑134 (MQ=255)
           ttAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACg                                                                                                                         <  1:159238/139‑1 (MQ=255)
                 atGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTaa                                                                                                                   <  1:1291803/139‑1 (MQ=255)
                   gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAAtt                                                                                                                 >  2:679687/1‑139 (MQ=255)
                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATtc                                                                                                                >  2:696517/1‑139 (MQ=255)
                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                               >  1:275866/1‑123 (MQ=255)
                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                               <  2:275866/123‑1 (MQ=255)
                     cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGTAGCAAATTACCCCCCCAGACGAGGTAATtcat                                                                                                              >  2:528998/1‑139 (MQ=255)
                       ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATtcatc                                                                                                             >  1:1257723/1‑139 (MQ=255)
                        tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                               <  1:297684/119‑1 (MQ=255)
                        tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                               >  2:297684/1‑119 (MQ=255)
                          ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCatcaat                                                                                                          >  2:299683/1‑139 (MQ=255)
                               accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCAttt                                                                                                     >  2:192373/1‑139 (MQ=255)
                                        ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          <  2:705012/108‑5 (MQ=255)
                                        ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          >  1:705012/1‑104 (MQ=255)
                                         tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa                                                                                           >  2:385958/1‑139 (MQ=255)
                                         tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa                                                                                           <  2:1257723/139‑1 (MQ=255)
                                         tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTCACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa                                                                                           <  1:696517/139‑1 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                    >  2:1130706/1‑139 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                    <  2:929675/139‑1 (MQ=255)
                                                     ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt                                                                               >  2:313665/1‑139 (MQ=255)
                                                      tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt                                                                              >  2:372166/1‑139 (MQ=255)
                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:372166/139‑1 (MQ=255)
                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:299683/139‑1 (MQ=255)
                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:385958/139‑1 (MQ=255)
                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCCCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:285184/139‑1 (MQ=255)
                                                          gcgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccccca                                                                                                                            >  1:1137236/1‑86 (MQ=255)
                                                          gcgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccccca                                                                                                                            <  2:1137236/88‑3 (MQ=255)
                                                                    ccaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                <  1:695817/139‑1 (MQ=255)
                                                                    ccaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                <  2:321009/139‑1 (MQ=255)
                                                                      accCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCatcaa                                                                                                           >  2:708943/1‑94 (MQ=255)
                                                                      accCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGCGTAGCAAATTACCCCCCCAGACGAGGTAATTCatcaa                                                                                                           <  1:708943/94‑1 (MQ=255)
                                                                        ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 <  1:75894/134‑1 (MQ=255)
                                                                        ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 >  2:75894/1‑134 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  2:1110813/1‑139 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  2:721114/1‑139 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  2:154578/1‑139 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  1:314919/1‑139 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          >  2:164740/1‑70 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          <  1:164740/74‑5 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                               <  2:45814/69‑1 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                               >  1:45814/1‑69 (MQ=255)
                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                     <  1:172668/139‑1 (MQ=255)
                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                     <  2:486664/139‑1 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTGAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                  >  2:780543/1‑139 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                             >  1:327498/1‑96 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                             <  2:327498/96‑1 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                  >  2:1081357/1‑139 (MQ=255)
                                                                                    aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  2:1129408/111‑1 (MQ=255)
                                                                                    aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            >  1:1129408/1‑111 (MQ=255)
                                                                                     aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          <  1:221315/63‑5 (MQ=255)
                                                                                     aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                          >  2:221315/1‑59 (MQ=255)
                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                              >  1:946016/1‑139 (MQ=255)
                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                              >  2:351479/1‑139 (MQ=255)
                                                                                        cGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc                                                                                   <  2:1154939/100‑1 (MQ=255)
                                                                                        cGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc                                                                                   >  1:1154939/1‑100 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                       <  2:888858/47‑1 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                       >  1:888858/1‑47 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAgg                                                                                                                      >  2:413216/1‑62 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAgg                                                                                                                      <  1:413216/62‑1 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATTAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc                                                   <  2:772111/129‑1 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 >  2:803352/1‑115 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 <  1:803352/115‑1 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc                                                   >  1:772111/1‑129 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                         <  1:1130706/139‑1 (MQ=255)
                                                                                           ccGCTTACCTCACATAAACCAGTAAATATTGCTCTGGTTACAAATTACACCCCCAAACTATCTAATTCATCAAGCTTTTCCAAGTGCAACAATTATCGCTTGTACAAATTTACATCAGGCATTAGCATCCGTTTCATCt                                         <  2:1244140/139‑1 (MQ=255)
                                                                                               ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                     <  2:1025255/139‑1 (MQ=255)
                                                                                                tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca                                    >  1:1208738/1‑139 (MQ=255)
                                                                                                 aCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac                                   <  2:1208738/139‑1 (MQ=255)
                                                                                                  ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  <  1:351479/139‑1 (MQ=255)
                                                                                                  ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  <  1:192373/139‑1 (MQ=255)
                                                                                                    tCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAAt                                <  1:313665/139‑1 (MQ=255)
                                                                                                     cACCAAAACCAGTAAATATTGCTCGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                              <  1:528998/139‑1 (MQ=255)
                                                                                                      aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                     >  1:1078401/1‑132 (MQ=255)
                                                                                                      aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                     <  2:1078401/132‑1 (MQ=255)
                                                                                                       ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                              >  2:60318/1‑74 (MQ=255)
                                                                                                       ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                              <  1:60318/74‑1 (MQ=255)
                                                                                                             ccAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                              >  2:1297642/1‑132 (MQ=255)
                                                                                                             ccAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                              <  1:1297642/132‑1 (MQ=255)
                                                                                                                   aaTATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgt                 <  1:780543/139‑1 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa            >  2:176866/1‑139 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  >  1:945623/1‑115 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  <  2:945623/115‑1 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca          <  2:314919/139‑1 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca          <  2:382946/139‑1 (MQ=255)
                                                                                                                          cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAACATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca          >  2:1027139/1‑139 (MQ=255)
                                                                                                                               gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt                    >  1:19184/1‑124 (MQ=255)
                                                                                                                               gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt                    <  2:19184/124‑1 (MQ=255)
                                                                                                                                 aGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc   <  1:154578/139‑1 (MQ=255)
                                                                                                                                  gCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc  >  1:705574/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                    
TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC  >  NC_000913/2484754‑2485021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: