Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	302,193	(C)5→6	coding (381/690 nt)	paoA ←	PaoABC aldehyde oxidoreductase, 2Fe‑2S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	302,188	1	.	C	100.0%	168.6 / NA	49	A129G (GCC→GGC)	paoA	PaoABC aldehyde oxidoreductase, 2Fe‑2S subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (25/24);  total (25/24)

CTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑CCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCA  >  NC_000913/302051‑302317                                                                                                                                           |                                                                                                                                  ctTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑c                                                                                                                                  >  1:992013/1‑139 (MQ=255)          ccGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑cccccctgat                                                                                                                         >  1:388366/1‑134 (MQ=255)           cGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATg                                                                                                                         <  2:388366/139‑1 (MQ=255)                        cAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGc                                                                                                            >  1:884219/1‑139 (MQ=255)                          aaTTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGt                                                                                                          <  2:992013/139‑1 (MQ=255)                                  cGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTcaggcagg                                                                                                  >  2:1020023/1‑139 (MQ=255)                                    gAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAggcaggca                                                                                                >  1:754976/1‑139 (MQ=255)                                     aGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAt                                                                                               >  2:1298969/1‑139 (MQ=255)                                        tGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTaa                                                                                            >  1:1273390/1‑139 (MQ=255)                                              aGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGc                                                                                      <  2:754976/139‑1 (MQ=255)                                              aGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGc                                                                                      <  1:1298969/139‑1 (MQ=255)                                               gCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCg                                                                                     >  2:437556/1‑139 (MQ=255)                                               gCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑c                                                                                                                                  <  2:1015993/93‑1 (MQ=255)                                               gCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑c                                                                                                                                  >  1:1015993/1‑93 (MQ=255)                                                        gAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGGATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGAc                                                                            <  2:335206/139‑1 (MQ=255)                                                            ccATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGc                                                                        <  2:687903/139‑1 (MQ=255)                                                             catcatGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTcaggca                                                                                                    >  2:692660/1‑110 (MQ=255)                                                             catcatGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTcaggca                                                                                                    <  1:692660/110‑1 (MQ=255)                                                                         tAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTcaggca                                                                                                    >  1:410675/1‑98 (MQ=255)                                                                         tAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTcaggca                                                                                                    <  2:410675/98‑1 (MQ=255)                                                                         tAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTc                                                           >  1:140581/1‑139 (MQ=255)                                                                             ggCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCa                                                       <  2:1273390/139‑1 (MQ=255)                                                                             ggCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCa                                                       <  2:228100/139‑1 (MQ=255)                                                                              gccgccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCAc                                                      >  1:721118/1‑139 (MQ=255)                                                                                 gccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGc                                                                                                                >  2:188926/1‑78 (MQ=255)                                                                                 gccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGc                                                                                                                <  1:188926/78‑1 (MQ=255)                                                                                  ccTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTc                                                  >  1:317352/1‑139 (MQ=255)                                                                                   cTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGAc                                                                            <  2:656454/112‑1 (MQ=255)                                                                                   cTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGAc                                                                            >  1:656454/1‑112 (MQ=255)                                                                                   cTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCa                                                                       >  2:635524/1‑117 (MQ=255)                                                                                   cTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCa                                                                       <  1:635524/117‑1 (MQ=255)                                                                                       aTGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGAc                                                                                   <  1:1034704/101‑1 (MQ=255)                                                                                       aTGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGAc                                                                                   >  2:1034704/1‑101 (MQ=255)                                                                                               aaGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGCAAcc                                     >  2:110496/1‑139 (MQ=255)                                                                                                 gATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGt                                                                                                          <  1:393232/68‑1 (MQ=255)                                                                                                 gATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGt                                                                                                          >  2:393232/1‑68 (MQ=255)                                                                                                     aTCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCa                                                       <  1:318055/115‑1 (MQ=255)                                                                                                     aTCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCa                                                       >  2:318055/1‑115 (MQ=255)                                                                                                      tCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑cc                                                                                                                                 >  1:34686/1‑39 (MQ=255)                                                                                                      tCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑cc                                                                                                                                 <  2:34686/39‑1 (MQ=255)                                                                                                         ggCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAtt                                                                                              <  1:826604/72‑1 (MQ=255)                                                                                                         ggCGAACCCAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCAtt                                                                                              >  2:826604/1‑72 (MQ=255)                                                                                                               cccAGGCCTTCAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATc                     >  1:678479/1‑139 (MQ=255)                                                                                                                         cAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCAc                                                      >  1:817324/1‑96 (MQ=255)                                                                                                                         cAATGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCAc                                                      <  2:817324/96‑1 (MQ=255)                                                                                                                          aaTGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGc                                         >  2:312693/1‑108 (MQ=255)                                                                                                                          aaTGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGc                                         <  1:312693/108‑1 (MQ=255)                                                                                                                          aaTGGTGGTGATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGAtt          <  1:110496/139‑1 (MQ=255)                                                                                                                                 gtgATCTCGGCCCCCCTGATGCATGACTGCAAGCGt                                                                                                          >  1:1287216/1‑36 (MQ=255)                                                                                                                                 gtgATCTCGGCCCCCCTGATGCATGACTGCAAGCGt                                                                                                          <  2:1287216/36‑1 (MQ=255)                                                                                                                                 gtgATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGc                                                             >  1:637012/1‑81 (MQ=255)                                                                                                                                 gtgATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGc                                                             <  2:637012/81‑1 (MQ=255)                                                                                                                                  tgATCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCa  <  2:721118/139‑1 (MQ=255)                                                                                                                                    aTCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCa                                                       >  1:674476/1‑84 (MQ=255)                                                                                                                                    aTCTCGGCCCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCa                                                       <  2:674476/84‑1 (MQ=255)                                                                                                                                           |                                                                                                                                  CTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACCCAGGCCTTCAATGGTGGTGATCTCGG‑CCCCCTGATGCATGACTGCAAGCGTCAGGCAGGCATTAAGCCTGCGACCATTGACCAGCACGGTACAGGCTCCGCACTGTCCGTGATCGCAACCTTTCTTGGTACCGATCAAATGCAGATTTTCACGCA  >  NC_000913/302051‑302317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑