Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,474,138 (A)6→7 coding (15/594 nt) bdcR → transcriptional repressor for divergent bdcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,474,1321.A98.7% 253.0 / ‑4.1 78coding (9/594 nt)bdcRtranscriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (44/33);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTA  >  NC_000913/4474000‑4474265
                                                                                                                                     |                                                                                                                                      
gATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaaaaaa                                                                                                                                  >  2:928807/1‑139 (MQ=255)
        gACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                          <  2:681941/139‑1 (MQ=255)
         aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                         >  2:926852/1‑139 (MQ=255)
                 cGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAg                                                                                                                 >  1:929339/1‑139 (MQ=255)
                            aCTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                      <  1:928807/139‑1 (MQ=255)
                                tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGtt                                                                                                  >  2:545704/1‑139 (MQ=255)
                                 cTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                 <  2:975679/139‑1 (MQ=255)
                                     ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                             >  1:1084954/1‑139 (MQ=255)
                                                gcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGc                                                                                  >  1:594156/1‑139 (MQ=255)
                                                 cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                 <  2:560652/123‑1 (MQ=255)
                                                 cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                 >  1:560652/1‑123 (MQ=255)
                                                   cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                               <  1:926852/139‑1 (MQ=255)
                                                              cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                    >  1:199741/1‑139 (MQ=255)
                                                              cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                    >  2:617539/1‑139 (MQ=255)
                                                                      attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtt                                                            >  1:616533/1‑139 (MQ=255)
                                                                      attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtt                                                            >  1:1087998/1‑139 (MQ=255)
                                                                      attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtt                                                            >  2:347154/1‑139 (MQ=255)
                                                                      attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtt                                                            >  2:1119223/1‑139 (MQ=255)
                                                                               cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                         >  1:1027847/1‑69 (MQ=255)
                                                                               cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                         <  2:1027847/69‑1 (MQ=255)
                                                                               cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa                                                   >  1:651366/1‑139 (MQ=255)
                                                                                 tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCGGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                 >  1:174174/1‑139 (MQ=255)
                                                                                 tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                 >  2:22327/1‑139 (MQ=255)
                                                                                 tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                 >  1:1156409/1‑139 (MQ=255)
                                                                                 tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                 >  1:500120/1‑139 (MQ=255)
                                                                                 tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                 >  1:460866/1‑139 (MQ=255)
                                                                                 tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                 >  1:223300/1‑139 (MQ=255)
                                                                                  gCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                            >  1:811833/1‑95 (MQ=255)
                                                                                  gCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                            <  2:811833/95‑1 (MQ=255)
                                                                                   cATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAg                                               >  2:612901/1‑139 (MQ=255)
                                                                                    aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACg                                                                                                    >  2:161329/1‑85 (MQ=255)
                                                                                    aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACg                                                                                                    <  1:161329/85‑1 (MQ=255)
                                                                                      tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                  <  2:924718/133‑1 (MQ=255)
                                                                                      tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                  >  1:924718/1‑133 (MQ=255)
                                                                                      tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                            >  1:107998/1‑139 (MQ=255)
                                                                                      tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                            >  1:1228801/1‑139 (MQ=255)
                                                                                        cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                    <  2:1136054/113‑1 (MQ=255)
                                                                                        cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                    >  1:1136054/1‑113 (MQ=255)
                                                                                        cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAg                                                                 >  2:834679/1‑116 (MQ=255)
                                                                                        cAAAACTGTTTTAACTTTCTGTCAACACCTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGATCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAg                                                                 <  1:834679/116‑1 (MQ=255)
                                                                                        cAAAACTGTTTTAACTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCg                                         <  1:1302117/139‑1 (MQ=255)
                                                                                         aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                       >  2:703114/1‑93 (MQ=255)
                                                                                         aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                       <  1:703114/93‑1 (MQ=255)
                                                                                          aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                        <  1:617539/139‑1 (MQ=255)
                                                                                          aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                        <  2:199741/139‑1 (MQ=255)
                                                                                          aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                        <  1:1119223/139‑1 (MQ=255)
                                                                                           aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCTAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAATGTTTCGAt                                       >  1:780313/1‑139 (MQ=255)
                                                                                             cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                       >  1:620683/1‑137 (MQ=255)
                                                                                             cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                       <  2:620683/137‑1 (MQ=255)
                                                                                              tGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCt                                    <  2:500120/139‑1 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                            <  2:635098/81‑1 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                            >  1:635098/1‑81 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAtctc                                                                            <  1:172700/97‑1 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                               <  2:861596/94‑1 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                               >  1:861596/1‑94 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                  >  2:230834/1‑139 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                  >  2:815781/1‑139 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                  >  1:771114/1‑139 (MQ=255)
                                                                                                ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACCAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAtctc                                                                            >  2:172700/1‑97 (MQ=255)
                                                                                                   tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg                               <  2:260877/139‑1 (MQ=255)
                                                                                                          cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                       >  1:885929/1‑76 (MQ=255)
                                                                                                          cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                       <  2:885929/76‑1 (MQ=255)
                                                                                                               aaCAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt                   <  1:230834/139‑1 (MQ=255)
                                                                                                               aaCAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt                   <  1:612901/139‑1 (MQ=255)
                                                                                                                 cAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTa                 <  2:107998/139‑1 (MQ=255)
                                                                                                                     ttttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa             <  2:594156/139‑1 (MQ=255)
                                                                                                                     ttttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa             <  2:771114/139‑1 (MQ=255)
                                                                                                                       ttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt           >  1:378429/1‑139 (MQ=255)
                                                                                                                          tAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc        <  1:22327/139‑1 (MQ=255)
                                                                                                                          tAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc        <  2:616533/139‑1 (MQ=255)
                                                                                                                              ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                 <  2:1162365/46‑1 (MQ=255)
                                                                                                                              ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                 >  1:1162365/1‑46 (MQ=255)
                                                                                                                              ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt                   >  2:1023202/1‑124 (MQ=255)
                                                                                                                              ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt                   <  1:1023202/124‑1 (MQ=255)
                                                                                                                              ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg    >  2:1214609/1‑139 (MQ=255)
                                                                                                                              ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg    >  1:408221/1‑139 (MQ=255)
                                                                                                                              ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg    >  1:1161857/1‑139 (MQ=255)
                                                                                                                               gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGt   >  1:972190/1‑139 (MQ=255)
                                                                                                                                tCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTa  <  2:972190/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTA  >  NC_000913/4474000‑4474265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: