Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	825,744	T→C	D122G (GAC→GGC)	ybhR ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	825,744	0	T	C	100.0%	200.6 / NA	55	D122G (GAC→GGC)	ybhR	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

TTGCGTACCACCAGCTCGCTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGTCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGCTTTTCAGCAGCAG  >  NC_000913/825621‑825877                                                                                                                            |                                                                                                                                      ttGCGTACCACCAGCTCGCTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCa                                                                                                                        >  2:1120057/1‑139 (MQ=255)     gTACCACCAGCTCGCTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAgg                                                                                                                    <  2:1096706/139‑1 (MQ=255)       accaccAGCTCGCTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGTTCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGgcg                                                                                                                  <  1:48163/139‑1 (MQ=255)               cTCGCTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTgg                                                                                                          >  1:417802/1‑139 (MQ=255)                tCGCTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGa                                                                                                         <  1:649906/139‑1 (MQ=255)                   cTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGGTAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCATAGgcg                                                                                                                  >  1:807594/1‑127 (MQ=255)                   cTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGgcg                                                                                                                  <  2:807594/127‑1 (MQ=255)                            ggTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGtt                                                                                             <  2:574954/139‑1 (MQ=255)                                      ttccttccAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGgcgc                                                                                                                 >  1:151775/1‑109 (MQ=255)                                      ttccttccAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGgcgc                                                                                                                 <  2:151775/109‑1 (MQ=255)                                       tccttccAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAg                                                                                  >  2:307754/1‑139 (MQ=255)                                                 agcTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGa                                                                        <  1:1120057/139‑1 (MQ=255)                                                   cTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGaaa                                                                      >  2:801764/1‑139 (MQ=255)                                                   cTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGaaa                                                                      <  1:307754/139‑1 (MQ=255)                                                     ccTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACg                                                                    <  2:1025608/139‑1 (MQ=255)                                                            aTAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGt                                                                                 <  1:958431/119‑1 (MQ=255)                                                            aTAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGt                                                                                 >  2:958431/1‑119 (MQ=255)                                                              aaTTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCagtag                                                           >  1:321618/1‑139 (MQ=255)                                                                      aCGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACg                                                                    <  2:215408/122‑1 (MQ=255)                                                                      aCGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACg                                                                    >  1:215408/1‑122 (MQ=255)                                                                          tCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTtg                                               <  2:134824/139‑1 (MQ=255)                                                                                gCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTc                                         <  2:321618/139‑1 (MQ=255)                                                                                   ggTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCagtagt                                                          >  2:1130756/1‑119 (MQ=255)                                                                                   ggTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCagtagt                                                          <  1:1130756/119‑1 (MQ=255)                                                                                    gTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATg                                     >  1:341731/1‑139 (MQ=255)                                                                                    gTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGACGATg                                     >  1:362955/1‑139 (MQ=255)                                                                                      aGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTtgt                                              >  2:96600/1‑128 (MQ=255)                                                                                      aGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTtgt                                              <  1:96600/128‑1 (MQ=255)                                                                                       gTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGtt                                  >  1:166718/1‑139 (MQ=255)                                                                                        ttGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGgcgc                                                                                                                 >  1:70609/1‑59 (MQ=255)                                                                                        ttGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGgcgc                                                                                                                 <  2:70609/59‑1 (MQ=255)                                                                                         tGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGaaa                                                                      >  2:596452/1‑101 (MQ=255)                                                                                         tGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGaaa                                                                      <  1:596452/101‑1 (MQ=255)                                                                                          ggcggcAATTTGCGCACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTggg                               <  2:805387/139‑1 (MQ=255)                                                                                                      cgcACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATgg                                    >  1:1309015/1‑122 (MQ=255)                                                                                                      cgcACTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATgg                                    <  2:1309015/122‑1 (MQ=255)                                                                                                         aCTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTggg                <  2:417802/139‑1 (MQ=255)                                                                                                          cTGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGc               <  2:166718/139‑1 (MQ=255)                                                                                                           tGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCtgtg                  >  1:63915/1‑135 (MQ=255)                                                                                                           tGTTGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCtgtg                  <  2:63915/135‑1 (MQ=255)                                                                                                             ttGGAGTTACGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGCttt            >  1:10405/1‑139 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCagtagt                                                          <  2:476419/85‑1 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCagtagt                                                          >  1:476419/1‑85 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGAt                          <  1:621311/117‑1 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGAt                          >  2:1190286/1‑117 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGAt                          <  1:1190286/117‑1 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGAt                          <  2:848780/117‑1 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGAt                          >  1:848780/1‑117 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGCTTTTcagcagc    >  1:322995/1‑139 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGCTTTTcagcagc    >  1:829907/1‑139 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGCTTTTcagcagc    >  1:1185377/1‑139 (MQ=255)                                                                                                                     aCGCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGACTTTTGTGTGTCGATGGTTGGGCGGAt                          >  2:621311/1‑117 (MQ=255)                                                                                                                       gCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACg                                                                    >  2:670827/1‑73 (MQ=255)                                                                                                                       gCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACg                                                                    <  1:670827/73‑1 (MQ=255)                                                                                                                       gCCCGCCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGCTTTTcagcagcag  >  2:17562/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                      TTGCGTACCACCAGCTCGCTGTTGTTAGGTTTCGGTTTTCCTTCCAGCAGCTCCTGCTGATAATTTTTGACGATCTGTTGCAGGTAGTTGGCGGCAATTTGCGCACTGTTGGAGTTACGCCCGTCGAGGATCAACTGCAAAGGCGCGGTCTGGAAGGTATCCAGTTTGCGCGAGAAGTCAGCCGGGAAACGCACCAGTAGTAACGCCTTTTGTGTGTCGATGGTTGGGCGGATCTCCTGTGGGCTTTTCAGCAGCAG  >  NC_000913/825621‑825877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑