Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	879,857	G→A	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI → gstB ←	soluble aldose sugar dehydrogenase glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	879,857	0	G	A	100.0%	174.6 / NA	55	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI gstB	soluble aldose sugar dehydrogenase glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

AAGTGACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879721‑879987                                                                                                                                         |                                                                                                                                   aaGTGACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACt                                                                                                                                  <  2:159251/139‑1 (MQ=255)      aCAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACgg                                                                                                                             >  1:489114/1‑139 (MQ=255)          aagaTGGCCGTATTTTAACGGACAGAGGGCAGCGAATTTGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAat                                                                                                                         <  2:369039/139‑1 (MQ=255)              tGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAatcatc                                                                                                                     >  2:1023580/1‑139 (MQ=255)              tGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCGCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGAAatcatc                                                                                                                     >  2:431405/1‑139 (MQ=255)                                   agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATcacc                                                                                                                  <  1:133551/121‑1 (MQ=255)                                   agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATcacc                                                                                                                  >  2:133551/1‑121 (MQ=255)                                         gCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCa                                                                                          <  2:489114/139‑1 (MQ=255)                                              ttCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAg                                                                                     <  2:493148/139‑1 (MQ=255)                                               tCGTGATGTTCGCACTGGACCCGACGGTTATTTATACTTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGt                                                                                    >  2:870229/1‑139 (MQ=255)                                                       ttCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACAAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATa                                                                            >  2:794842/1‑139 (MQ=255)                                                                   ccGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAg                                                                                     <  2:1218605/118‑1 (MQ=255)                                                                   ccGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAg                                                                                     >  1:1218605/1‑118 (MQ=255)                                                                      aCGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                        >  2:290052/1‑112 (MQ=255)                                                                      aCGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                        <  1:290052/112‑1 (MQ=255)                                                                          ttatttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACg                                                                                                                              <  1:806892/70‑1 (MQ=255)                                                                          ttatttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACg                                                                                                                              >  2:806892/1‑70 (MQ=255)                                                                           tatttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           <  2:1100781/88‑1 (MQ=255)                                                                           tatttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           >  1:1100781/1‑88 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:144697/139‑1 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:1194443/139‑1 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:1016530/139‑1 (MQ=255)                                                                              ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:746098/139‑1 (MQ=255)                                                                              ttatACGTTCTCACCGACAAGTCCAGTGGGGAATTTCTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:794842/139‑1 (MQ=255)                                                                                  aCGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGgtgt                                                 <  1:431405/139‑1 (MQ=255)                                                                                   cGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTc                                                >  2:139032/1‑139 (MQ=255)                                                                                     ttCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCa                                              >  1:252926/1‑139 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAAtcatca                                                                                                                    <  1:784908/68‑1 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAAtcatca                                                                                                                    >  2:784908/1‑68 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:205147/131‑1 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:205147/1‑131 (MQ=255)                                                                                      tctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAg                                             >  2:361591/1‑139 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGc                                                                                               <  2:895465/88‑1 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGc                                                                                               >  1:895465/1‑88 (MQ=255)                                                                                       ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAgg                                            >  1:368536/1‑139 (MQ=255)                                                                                        tcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           <  1:1023580/139‑1 (MQ=255)                                                                                        tcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           <  1:361591/139‑1 (MQ=255)                                                                                          aCCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCa                                         >  1:91927/1‑139 (MQ=255)                                                                                               cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:699163/122‑1 (MQ=255)                                                                                               cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTAAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:699163/1‑122 (MQ=255)                                                                                                  gTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  2:1190394/1‑119 (MQ=255)                                                                                                  gTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:1190394/119‑1 (MQ=255)                                                                                                             aaTTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           >  1:1314601/1‑54 (MQ=255)                                                                                                             aaTTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           <  2:1314601/54‑1 (MQ=255)                                                                                                                  cTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          >  2:423988/1‑50 (MQ=255)                                                                                                                  cTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                          <  1:423988/50‑1 (MQ=255)                                                                                                                      aaGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGaa             <  2:368536/139‑1 (MQ=255)                                                                                                                       aGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                >  1:786776/1‑55 (MQ=255)                                                                                                                       aGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                <  2:786776/55‑1 (MQ=255)                                                                                                                            gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           <  1:423107/103‑1 (MQ=255)                                                                                                                            gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           >  2:423107/1‑103 (MQ=255)                                                                                                                             cccACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcg      >  1:124756/1‑139 (MQ=255)                                                                                                                                 cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatag  >  1:179703/1‑139 (MQ=255)                                                                                                                                  gCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           >  2:1027251/1‑97 (MQ=255)                                                                                                                                  gCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGt                                           <  1:1027251/97‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   AAGTGACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879721‑879987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑