Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 911,132 A→G intergenic (‑83/+50) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913911,1320AG100.0% 168.2 / NA 46intergenic (‑83/+50)poxB/hcrpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

CCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATT  >  NC_000913/911004‑911266
                                                                                                                                |                                                                                                                                      
ccGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTa                                                                                                                              >  2:103537/1‑139 (MQ=255)
      cGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGc                                                                                                                        <  2:919592/139‑1 (MQ=255)
           gtTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGAt                                                                                                                   <  1:103537/139‑1 (MQ=255)
              ttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                                <  2:290011/139‑1 (MQ=255)
              ttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                                <  2:397315/139‑1 (MQ=255)
                      ataAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACgg                                                                                                                                 >  2:86463/1‑114 (MQ=255)
                      ataAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACgg                                                                                                                                 <  1:86463/114‑1 (MQ=255)
                              cAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGc                                                                                                                        >  1:1077235/1‑115 (MQ=255)
                              cAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGc                                                                                                                        <  2:1077235/115‑1 (MQ=255)
                              cAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGc                                                                                                >  1:1168423/1‑139 (MQ=255)
                              cAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGc                                                                                                >  1:401428/1‑139 (MQ=255)
                               aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa                                                                                               >  2:968584/1‑139 (MQ=255)
                               aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa                                                                                               >  1:642656/1‑139 (MQ=255)
                                   gtttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg                                                                                           <  1:153344/139‑1 (MQ=255)
                                    tttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAgg                                                                                          <  2:1117755/139‑1 (MQ=255)
                                     ttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                                >  1:64023/1‑116 (MQ=255)
                                     ttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                                <  2:64023/116‑1 (MQ=255)
                                                ttCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgag                                                                              <  2:401428/139‑1 (MQ=255)
                                                  ctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGaa                                                                            >  2:854410/1‑139 (MQ=255)
                                                                 gtgATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCg                                                             <  1:968584/139‑1 (MQ=255)
                                                                  tgATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg                                                            >  1:37641/1‑139 (MQ=255)
                                                                    aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                                >  1:501754/1‑85 (MQ=255)
                                                                    aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                                <  2:501754/85‑1 (MQ=255)
                                                                    aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa                                                                       >  2:1012789/1‑126 (MQ=255)
                                                                    aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa                                                                       <  1:1012789/126‑1 (MQ=255)
                                                                       aCGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                                       <  1:854410/139‑1 (MQ=255)
                                                                       aCGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                                       <  2:1168423/139‑1 (MQ=255)
                                                                        cGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt                                                                                                                                      <  1:7828/59‑1 (MQ=255)
                                                                            aaCAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg                                                  >  2:22018/1‑139 (MQ=255)
                                                                                 gTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc                                             <  2:159755/139‑1 (MQ=255)
                                                                                         cATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGt                                     <  2:642656/139‑1 (MQ=255)
                                                                                            cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat                                                                                                     <  1:452981/72‑1 (MQ=255)
                                                                                            cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat                                                                                                     >  2:452981/1‑72 (MQ=255)
                                                                                                    gggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTa                                                                                    <  1:305508/81‑1 (MQ=255)
                                                                                                    gggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTa                                                                                    >  2:305508/1‑81 (MQ=255)
                                                                                                     ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGa                         >  1:49285/1‑139 (MQ=255)
                                                                                                             gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCg                 >  1:231089/1‑139 (MQ=255)
                                                                                                               tGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa                                                                       >  1:1000380/1‑83 (MQ=255)
                                                                                                               tGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa                                                                       <  2:1000380/83‑1 (MQ=255)
                                                                                                               tGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                                       >  1:894255/1‑99 (MQ=255)
                                                                                                               tGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                                       <  2:894255/99‑1 (MQ=255)
                                                                                                                   agagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAata                                                                                                         <  2:262371/45‑1 (MQ=255)
                                                                                                                   agagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAata                                                                                                         >  1:262371/1‑45 (MQ=255)
                                                                                                                       aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCg                                                                                >  1:83644/1‑66 (MQ=255)
                                                                                                                       aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCg                                                                                <  2:83644/66‑1 (MQ=255)
                                                                                                                            aggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCAtt  <  2:37641/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                      
CCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATT  >  NC_000913/911004‑911266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: