Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 986,671 A→G R104R (CGT→CGC)  ompF ← outer membrane porin 1a (Ia,b,F)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913986,6710AG100.0% 217.9 / NA 61R104R (CGT→CGC) ompFouter membrane porin 1a (Ia,b,F)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/26);  total (35/26)

ATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGG  >  NC_000913/986539‑986801
                                                                                                                                    |                                                                                                                                  
aTCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGtt                                                                                                                              >  1:142557/1‑139 (MQ=255)
   gctgtatgctgtatCACCACCAAATTCTGGCAGCCTATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTg                                                                                                                           <  1:841641/139‑1 (MQ=255)
               aTCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGa                                                                                                               >  1:1228097/1‑139 (MQ=255)
                 caccacCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGc                                                                                                             >  2:840240/1‑139 (MQ=255)
                   ccaccaAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGt                                                                                                           >  1:330803/1‑139 (MQ=255)
                                             gtAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTgg                                                                                 <  2:330803/139‑1 (MQ=255)
                                                aCCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGtt                                                                                                                         >  1:725574/1‑96 (MQ=255)
                                                aCCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGtt                                                                                                                         <  2:725574/96‑1 (MQ=255)
                                                 cccAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGt                                                                             >  2:132861/1‑139 (MQ=255)
                                                 cccAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGt                                                                             >  2:398022/1‑139 (MQ=255)
                                                      tGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTgg                                                                                 <  2:111830/130‑1 (MQ=255)
                                                      tGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTgg                                                                                 >  1:111830/1‑130 (MQ=255)
                                                      tGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTatat                                                                        >  1:133155/1‑139 (MQ=255)
                                                      tGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTatat                                                                        >  1:877820/1‑139 (MQ=255)
                                                      tGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTatat                                                                        >  1:1091575/1‑139 (MQ=255)
                                                           catAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCATGGAAGTTATATTCCCa                                                                   <  2:751821/139‑1 (MQ=255)
                                                                aCCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGAc                                                              <  1:132861/139‑1 (MQ=255)
                                                                     acCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATaa                                                         >  1:494698/1‑139 (MQ=255)
                                                                     acCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATaa                                                         >  1:946421/1‑139 (MQ=255)
                                                                      ccGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAAc                                                        >  1:24423/1‑139 (MQ=255)
                                                                       cGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCa                                                                   >  1:246664/1‑127 (MQ=255)
                                                                       cGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCa                                                                   <  2:246664/127‑1 (MQ=255)
                                                                         tAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCa                                                                   <  2:1008114/125‑1 (MQ=255)
                                                                         tAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCa                                                                   >  1:1008114/1‑125 (MQ=255)
                                                                         tAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCgg                                                     >  1:498675/1‑139 (MQ=255)
                                                                          aGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGt                                                    >  1:688400/1‑139 (MQ=255)
                                                                            ttACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGt                                                                                                                               >  1:832088/1‑62 (MQ=255)
                                                                            ttACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGt                                                                                                                               <  2:832088/62‑1 (MQ=255)
                                                                            ttACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCa                                                  <  1:840240/139‑1 (MQ=255)
                                                                                 gCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCa                                                                                                         <  1:910513/79‑1 (MQ=255)
                                                                                 gCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCa                                                                                                         >  2:910513/1‑79 (MQ=255)
                                                                                 gCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGAt                                               >  1:1001311/1‑137 (MQ=255)
                                                                                 gCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGAt                                               <  2:1001311/137‑1 (MQ=255)
                                                                                   cGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGt                                                                                                                   >  2:56501/1‑67 (MQ=255)
                                                                                   cGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGt                                                                                                                   <  1:56501/67‑1 (MQ=255)
                                                                                   cGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTATCCTGGAAGTTATATTCCCa                                                                   >  1:573277/1‑115 (MQ=255)
                                                                                   cGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTATCCTGGAAGTTATATTCCCa                                                                   <  2:573277/115‑1 (MQ=255)
                                                                                      aaTCGTAAGAACCAACGTCAGCG‑ATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCa                                                                                                <  2:940537/82‑1 (MQ=255)
                                                                                      aaTCGAAAGAACCAACGTCAGCG‑ATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCa                                                                                                >  1:940537/1‑82 (MQ=255)
                                                                                         cGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGAt                                     <  2:1091575/139‑1 (MQ=255)
                                                                                          gAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGAtt                                    <  1:398022/139‑1 (MQ=255)
                                                                                            aaGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTg                                  >  1:860969/1‑139 (MQ=255)
                                                                                                         aGCGTATTTAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTa                     >  2:1177319/1‑139 (MQ=255)
                                                                                                                ttAAGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCaa              >  2:988961/1‑139 (MQ=255)
                                                                                                                  aaGACCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCCTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCACCTTTAAAACCAAGa            <  2:877820/139‑1 (MQ=255)
                                                                                                                     aCCCGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACgg         >  1:1119381/1‑139 (MQ=255)
                                                                                                                      cccGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAgc                                                                                                       >  2:122891/1‑44 (MQ=255)
                                                                                                                      cccGCGAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAgc                                                                                                       <  1:122891/44‑1 (MQ=255)
                                                                                                                        cgcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTgg                                                                                 <  2:815568/64‑1 (MQ=255)
                                                                                                                        cgcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGAc                                                              <  1:689556/83‑1 (MQ=255)
                                                                                                                        cgcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGAc                                                              >  2:689556/1‑83 (MQ=255)
                                                                                                                        cgcgAATGCCAGGCGCGTTTTGTTACCACTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTgg                                                                                 >  1:815568/1‑64 (MQ=255)
                                                                                                                         gcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCa                                                                                                <  1:759750/48‑1 (MQ=255)
                                                                                                                         gcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAgttg                                                                                          <  2:216220/54‑1 (MQ=255)
                                                                                                                         gcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAgttg                                                                                          <  1:1212111/54‑1 (MQ=255)
                                                                                                                         gcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAgttg                                                                                          >  1:216220/1‑54 (MQ=255)
                                                                                                                         gcgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAgttg                                                                                          >  2:1212111/1‑54 (MQ=255)
                                                                                                                         gcgAATGCCAGGCGCGTTTTGTTACCAGTGTGAGCGTCAGCGCCTGCa                                                                                                >  2:759750/1‑48 (MQ=255)
                                                                                                                          cgAATGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATa    >  1:395216/1‑139 (MQ=255)
                                                                                                                           gAATGCCAGGCGCGTTTTGCTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACTGGCATAg   <  2:860969/139‑1 (MQ=255)
                                                                                                                            aaTGCCAGGCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAgg  <  1:351980/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
ATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGG  >  NC_000913/986539‑986801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑