Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,056,923	A→G	E221E (GAA→GAG)	torT →	periplasmic sensory protein associated with the TorRS two‑component regulatory system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,056,923	0	A	G	100.0%	180.4 / NA	49	E221E (GAA→GAG)	torT	periplasmic sensory protein associated with the TorRS two‑component regulatory system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

ACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAACGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCT  >  NC_000913/1056790‑1057054                                                                                                                                      |                                                                                                                                    aCGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCa                                                                                                                                <  1:467481/139‑1 (MQ=255)     cGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCa                                                                                                                            >  1:495792/1‑139 (MQ=255)                   agaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCg                                                                                                                     <  2:382725/132‑1 (MQ=255)                   agaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCg                                                                                                                     >  1:382725/1‑132 (MQ=255)                    gaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGtt                                                                                                             >  1:831161/1‑139 (MQ=255)                    gaTGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGtt                                                                                                             <  1:518124/139‑1 (MQ=255)                         tCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCgg                                                                                                        <  2:831161/139‑1 (MQ=255)                            aGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAAc                                                                                                     >  2:4102/1‑139 (MQ=255)                                         aGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCa                                                                                        <  2:495792/139‑1 (MQ=255)                                          gCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCa                                                                                        >  1:87533/1‑138 (MQ=255)                                          gCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCa                                                                                        <  2:87533/138‑1 (MQ=255)                                                               cGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGc                                                                  <  1:815462/139‑1 (MQ=255)                                                                               cgcTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAAcgcc                                                  >  1:1249253/1‑139 (MQ=255)                                                                               cgcTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAAcgcc                                                  <  2:1249253/139‑1 (MQ=255)                                                                                gcTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAAcgccg                                                 >  1:102647/1‑139 (MQ=255)                                                                                   tGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAggcg                                                                                  >  1:505859/1‑103 (MQ=255)                                                                                   tGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAggcg                                                                                  <  2:505859/103‑1 (MQ=255)                                                                                    ggTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCaa                                                                              <  2:745153/106‑1 (MQ=255)                                                                                    ggTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCaa                                                                              >  1:745153/1‑106 (MQ=255)                                                                                    ggTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTa                                             >  1:321757/1‑139 (MQ=255)                                                                                          aaCGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCgtgg                                       <  1:158210/139‑1 (MQ=255)                                                                                                   gAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGtttt                              >  1:179245/1‑139 (MQ=255)                                                                                                       tCCAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCt                          >  2:432453/1‑139 (MQ=255)                                                                                                         cAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGc                                                                  <  1:349399/97‑1 (MQ=255)                                                                                                         cAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGc                                                                  >  2:349399/1‑97 (MQ=255)                                                                                                         cAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCttt                        <  2:102647/139‑1 (MQ=255)                                                                                                         cAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCttt                        <  2:179245/139‑1 (MQ=255)                                                                                                         cAGCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCttt                        <  1:837732/139‑1 (MQ=255)                                                                                                           gCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGc                                                                                                  >  1:567867/1‑63 (MQ=255)                                                                                                           gCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGc                                                                                                  <  2:567867/63‑1 (MQ=255)                                                                                                           gCGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTca                      >  2:388075/1‑139 (MQ=255)                                                                                                            cGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAggcg                                                                                  <  2:57591/78‑1 (MQ=255)                                                                                                            cGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAggcg                                                                                  >  1:57591/1‑78 (MQ=255)                                                                                                            cGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGtt                                >  1:41059/1‑128 (MQ=255)                                                                                                            cGTAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGtt                                <  2:41059/128‑1 (MQ=255)                                                                                                              tAACCTGTTGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACAt                   <  2:628282/139‑1 (MQ=255)                                                                                                                      tGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCt                          >  2:737349/1‑124 (MQ=255)                                                                                                                      tGCAGGAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCt                          <  1:737349/124‑1 (MQ=255)                                                                                                                          ggAGATGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATcgc       >  2:827007/1‑139 (MQ=255)                                                                                                                           gagaTGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATcgcg      >  1:1002400/1‑139 (MQ=255)                                                                                                                             gaTGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGa                                                                       <  2:753417/72‑1 (MQ=255)                                                                                                                             gaTGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGa                                                                       >  1:753417/1‑72 (MQ=255)                                                                                                                               tGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAggc                                                                                   >  1:1206627/1‑58 (MQ=255)                                                                                                                               tGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAggc                                                                                   <  2:1206627/58‑1 (MQ=255)                                                                                                                               tGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAAc                                                     <  2:660268/88‑1 (MQ=255)                                                                                                                               tGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAAc                                                     >  1:660268/1‑88 (MQ=255)                                                                                                                               tGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCt                                               >  1:1241737/1‑94 (MQ=255)                                                                                                                               tGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCt                                               <  2:1241737/94‑1 (MQ=255)                                                                                                                               tGCTGGAGCGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCt  >  2:274435/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    ACGCCGGGGGCAGTAAGGAGATGGTCGAGGGTTTTCGCGCAGCCATTGCCGGAAGCCCGGTGCGTATTGTTGATATTGCGCTTGGTGATAACGATATTGAAATCCAGCGTAACCTGTTGCAGGAGATGCTGGAACGCCATCCAGAAATCGACGTCGTTGCCGGAACGGCCATTGCGGCAGAGGCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCT  >  NC_000913/1056790‑1057054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑