Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 104,352 A→G E124G (GAG→GGG)  ftsA → ATP‑binding cell division FtsK recruitment protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913104,3520AG100.0% 231.3 / NA 64E124G (GAG→GGG) ftsAATP‑binding cell division FtsK recruitment protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/32);  total (32/32)

TATATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCG  >  NC_000913/104217‑104485
                                                                                                                                       |                                                                                                                                     
tataTCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCa                                                                                                                                    <  1:885546/139‑1 (MQ=255)
   atCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCg                                                                                                                                 >  1:905367/1‑139 (MQ=255)
    tCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgt                                                                                                                                <  1:203260/139‑1 (MQ=255)
     cTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg                                                                                                                               <  1:369736/139‑1 (MQ=255)
       ggCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                             <  2:905367/139‑1 (MQ=255)
        gcgcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgc                                                                                                                            >  2:648065/1‑139 (MQ=255)
          gcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                             <  1:218000/136‑1 (MQ=255)
          gcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                             >  2:218000/1‑136 (MQ=255)
          gcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgctg                                                                                                                          >  2:864744/1‑139 (MQ=255)
          gcTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGtgctg                                                                                                                          >  2:458761/1‑139 (MQ=255)
            tttCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCg                                                                                                                                 <  1:1109199/130‑1 (MQ=255)
            tttCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCg                                                                                                                                 >  2:1109199/1‑130 (MQ=255)
             ttCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAt                                                                                                                       >  1:1200994/1‑139 (MQ=255)
                     aGCACATCAGCTGCCTGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATAACGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATccc                                                                                                               >  2:877539/1‑139 (MQ=255)
                             aGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGt                                                                                                       <  2:1157349/139‑1 (MQ=255)
                                 gCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGc                                                                                                   <  2:1231956/139‑1 (MQ=255)
                                 gCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGc                                                                                                   >  1:903129/1‑139 (MQ=255)
                                   cAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGa                                                                                                 <  1:648065/139‑1 (MQ=255)
                                    aGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAt                                                                                                >  1:968456/1‑139 (MQ=255)
                                     gAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAtt                                                                                               <  1:864744/139‑1 (MQ=255)
                                      aaTGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTg                                                                                              >  2:173424/1‑139 (MQ=255)
                                               ggTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAgg                                                                                     <  1:173424/139‑1 (MQ=255)
                                                 tATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                   <  1:111832/139‑1 (MQ=255)
                                                 tATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGaa                                                                                   <  1:458761/139‑1 (MQ=255)
                                                  aTGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg                                                                                                                               <  2:538086/94‑1 (MQ=255)
                                                  aTGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCgtg                                                                                                                               >  1:538086/1‑94 (MQ=255)
                                                     gTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                   <  1:714256/103‑1 (MQ=255)
                                                     gTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGa                                                                                                                   >  2:714256/1‑103 (MQ=255)
                                                     gTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGa                                                                               >  1:190116/1‑139 (MQ=255)
                                                        ccTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                             <  1:1177957/90‑1 (MQ=255)
                                                        ccTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                             >  2:1177957/1‑90 (MQ=255)
                                                                tGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                     >  1:787723/1‑74 (MQ=255)
                                                                tGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGc                                                                                                                                     <  2:787723/74‑1 (MQ=255)
                                                                tGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATC‑‑GTGCTGCATGTGATCCCGCAAGAGTATGCGa                                                                                                 >  2:489210/1‑108 (MQ=255)
                                                                tGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATC‑‑GTGCTGCATGTGATCCCGCAAGAGTATGCGa                                                                                                 <  1:489210/108‑1 (MQ=255)
                                                                   agaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                                 <  1:1295015/139‑1 (MQ=255)
                                                                    gaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAg                                                                >  2:603126/1‑139 (MQ=255)
                                                                       gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAtt                                                                                               >  1:824791/1‑105 (MQ=255)
                                                                       gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAtt                                                                                               <  2:824791/105‑1 (MQ=255)
                                                                       gaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAc                                                             >  2:1229183/1‑139 (MQ=255)
                                                                               gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtgt                                                                                                                     <  1:386881/75‑1 (MQ=255)
                                                                               gCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCAtgt                                                                                                                     >  2:386881/1‑75 (MQ=255)
                                                                                   gaagaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCaa                                                                                                           <  2:1148511/81‑1 (MQ=255)
                                                                                   gaagaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCaa                                                                                                           >  1:1148511/1‑81 (MQ=255)
                                                                                        tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATg                                            >  2:1172577/1‑139 (MQ=255)
                                                                                        tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATg                                            <  1:603126/139‑1 (MQ=255)
                                                                                           ggAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGTGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAg                                         >  1:247857/1‑139 (MQ=255)
                                                                                           ggAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                                 >  2:314473/1‑115 (MQ=255)
                                                                                           ggAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTa                                                                 <  1:314473/115‑1 (MQ=255)
                                                                                             aaaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGc                                       >  2:544486/1‑139 (MQ=255)
                                                                                             aaaaCGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGc                                       <  1:755646/139‑1 (MQ=255)
                                                                                                    cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                             <  2:404901/46‑1 (MQ=255)
                                                                                                    cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGtgtg                                                                                                                             >  1:404901/1‑46 (MQ=255)
                                                                                                       ccATACCGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAc                             <  2:903129/139‑1 (MQ=255)
                                                                                                            ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGc                                                     <  1:790863/110‑1 (MQ=255)
                                                                                                            ccGCGAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGc                                                     >  2:790863/1‑110 (MQ=255)
                                                                                                              gcgAAATCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca                      <  1:243191/139‑1 (MQ=255)
                                                                                                                 aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAt                                                                                                >  2:1173163/1‑62 (MQ=255)
                                                                                                                 aaaTCGGTGCGTGTGCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAt                                                                                                <  1:1173163/62‑1 (MQ=255)
                                                                                                                            tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCa                                                                                                            <  1:74946/39‑1 (MQ=255)
                                                                                                                            tgtgCGCGATGGGCATCGTGTGCTGCATGTGATCCCGCa                                                                                                            >  2:74946/1‑39 (MQ=255)
                                                                                                                                  cgATGGGCATCGTGTGCTGCATGTGATCCTGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGc                                       >  1:925979/1‑102 (MQ=255)
                                                                                                                                  cgATGGGCATCGTGTGCTGCATGTGATCCTGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGc                                       <  2:925979/102‑1 (MQ=255)
                                                                                                                                  cgATGGGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCg  >  1:226369/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TATATCTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCG  >  NC_000913/104217‑104485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑