Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,082,896	T→A	R218R (CGT→CGA)	efeO →	inactive ferrous ion transporter EfeUOB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,082,896	0	T	A	100.0%	147.0 / NA	46	R218R (CGT→CGA)	efeO	inactive ferrous ion transporter EfeUOB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/25);  total (21/25)

ACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCG  >  NC_000913/1082764‑1083026                                                                                                                                     |                                                                                                                                   aCCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGaa                                                                                                                              >  1:308302/1‑139 (MQ=255)      cgcgCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGc                                                                                                                         >  1:169870/1‑139 (MQ=255)       gcgcCAGCAATATGAGCGTATTGAACCAATTACTGAACTGTTCACCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCAGCCGTCCCAAACTTCCCTGGTTACCACCGACTGGAAAAAGCa                                                                                                                        <  2:870496/139‑1 (MQ=255)                  aTGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGa                                                                                                             <  2:169870/139‑1 (MQ=255)                        gTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAacac                                                                                                       <  1:1079117/139‑1 (MQ=255)                        gTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAacac                                                                                                       >  1:1037758/1‑139 (MQ=255)                         tATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAAcacc                                                                                                      <  1:334887/139‑1 (MQ=255)                           ttGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAAcaccac                                                                                                    >  2:77705/1‑139 (MQ=255)                           ttGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAAcaccac                                                                                                    >  2:610805/1‑139 (MQ=255)                           ttGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAAcaccac                                                                                                    >  1:628563/1‑139 (MQ=255)                                ccGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAg                                                                                               <  1:800763/139‑1 (MQ=255)                                ccGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAg                                                                                               >  2:392699/1‑139 (MQ=255)                                   aTTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGgga                                                                                            <  1:392699/139‑1 (MQ=255)                                   aTTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGgga                                                                                            <  2:1244096/139‑1 (MQ=255)                                        tGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatggat                                                                                       <  1:909482/139‑1 (MQ=255)                                          aaCTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCa                                                                                     <  1:77705/139‑1 (MQ=255)                                               ttCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGGGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACg                                                                                >  1:979166/1‑139 (MQ=255)                                                    cGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGag                                                                           <  2:995145/139‑1 (MQ=255)                                                     gATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                          <  2:308302/139‑1 (MQ=255)                                                         tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACt                                                                                                                                  >  2:525059/1‑79 (MQ=255)                                                         tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAgg                                                                                              >  2:892909/1‑115 (MQ=255)                                                         tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                          <  1:623765/135‑1 (MQ=255)                                                         tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                          >  2:623765/1‑135 (MQ=255)                                                         tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCt                                                                      >  1:791634/1‑139 (MQ=255)                                                         tGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTGGGCGACAACACCACCAGAgg                                                                                              <  1:892909/115‑1 (MQ=255)                                                                cagcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                          <  1:1206489/128‑1 (MQ=255)                                                                cagcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGagc                                                                          >  2:1206489/1‑128 (MQ=255)                                                                 agcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg                                                                                          <  1:589074/111‑1 (MQ=255)                                                                 agcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGggatg                                                                                          >  2:589074/1‑111 (MQ=255)                                                                 agcaTTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCg                                                              >  1:1149740/1‑139 (MQ=255)                                                                           cccGTGAAGATGATTACGGGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGa                                                    <  2:979166/139‑1 (MQ=255)                                                                             cGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCa                                                                                     <  2:347386/104‑1 (MQ=255)                                                                             cGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCa                                                                                     >  1:347386/1‑104 (MQ=255)                                                                                       atTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACg                                        <  2:1037758/139‑1 (MQ=255)                                                                                              gCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGt                                 >  2:407060/1‑139 (MQ=255)                                                                                                aaaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaaaaa                                          >  2:458503/1‑128 (MQ=255)                                                                                                aaaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCaaaaa                                          <  1:458503/128‑1 (MQ=255)                                                                                                 aaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAg                                                                                                                          <  2:24078/47‑1 (MQ=255)                                                                                                 aaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAg                                                                                                                          >  1:24078/1‑47 (MQ=255)                                                                                                 aaaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGaa                              <  2:791634/139‑1 (MQ=255)                                                                                                  aaaGCCGCCGACCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAAc                             <  1:407060/139‑1 (MQ=255)                                                                                                            aCCCAAAATTCACTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTCGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTccc                   <  2:1149740/139‑1 (MQ=255)                                                                                                                       aCTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGag                                                                           >  1:1143731/1‑72 (MQ=255)                                                                                                                       aCTGGTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGag                                                                           <  2:1143731/72‑1 (MQ=255)                                                                                                                          ggTTTCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTgg     >  2:1081692/1‑139 (MQ=255)                                                                                                                             ttCCACCGACTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTcg  <  1:875414/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   ACCGACGCGCCAGCACTATGAGCGTATTGAACCGATTGCTGAACTGTTCTCCGATCTGGATGGCAGCATTGACGCCCGTGAAGATGATTACGAGCAAAAAGCCGCCGACCCAAAATTCACTGGTTTCCACCGTCTGGAAAAAGCATTGTTTGGCGACAACACCACCAAAGGGATGGATCAGTACGCTGAGCAGCTTTATACCGATGTGGTCGATTTGCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCG  >  NC_000913/1082764‑1083026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑