Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,511	G→A	intergenic (‑24/+50)	dinI ← / ← pyrC	DNA damage‑inducible protein I/dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,511	0	G	A	100.0%	91.9 / NA	30	intergenic (‑24/+50)	dinI/pyrC	DNA damage‑inducible protein I/dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/10);  total (20/10)

TATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCT  >  NC_000913/1121382‑1121646                                                                                                                                  |                                                                                                                                        tATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGttatttat                                                                                                                                <  2:853380/139‑1 (MQ=255)       gAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTtata                                                                                                                               <  2:166725/134‑1 (MQ=255)       gAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTtata                                                                                                                               >  1:166725/1‑134 (MQ=255)                   gAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTaa                                                                                                              >  2:546690/1‑139 (MQ=255)                     aTACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATg                                                                                                            >  1:1183643/1‑139 (MQ=255)                     aTACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATg                                                                                                            >  1:1078316/1‑139 (MQ=255)                          gcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATAc                                                                                                                              <  2:795396/116‑1 (MQ=255)                          gcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACAAGTTATTTATAc                                                                                                                              >  1:795396/1‑116 (MQ=255)                           cggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGacaaca                                                                                                      >  2:1156928/1‑139 (MQ=255)                           cggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGacaaca                                                                                                      >  1:123694/1‑139 (MQ=255)                              gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAAc                                                                                                                  <  2:969915/124‑1 (MQ=255)                              gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAAc                                                                                                                  >  1:969915/1‑124 (MQ=255)                                                      aTAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACg                                                                           <  1:728627/139‑1 (MQ=255)                                                               gCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAAt                                                                                                                     <  1:162828/88‑1 (MQ=255)                                                               gCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAAt                                                                                                                     >  2:162828/1‑88 (MQ=255)                                                                     aaTGGAGAAGTTTTCGCTATGGTGACTTCAATTCGGATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGtt                                                            >  2:1292577/1‑139 (MQ=255)                                                                          agaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGa                                                                         <  1:1192902/121‑1 (MQ=255)                                                                          agaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGa                                                                         >  2:1192902/1‑121 (MQ=255)                                                                          agaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTcccc                                                       >  1:496681/1‑139 (MQ=255)                                                                            aaGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCgg                                                     <  1:546690/139‑1 (MQ=255)                                                                                             aCTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGc                                    >  2:256874/1‑139 (MQ=255)                                                                                                    ttCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAg                                                                     >  2:1171981/1‑99 (MQ=255)                                                                                                    ttCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAg                                                                     <  1:1171981/99‑1 (MQ=255)                                                                                                              aGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGc                   >  2:1071759/1‑139 (MQ=255)                                                                                                                  cccTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATg               >  1:618133/1‑139 (MQ=255)                                                                                                                   ccTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCg                                   >  2:1128687/1‑118 (MQ=255)                                                                                                                   ccTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCg                                   <  1:1128687/118‑1 (MQ=255)                                                                                                                   ccTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGc              >  2:370695/1‑139 (MQ=255)                                                                                                                     tGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCtt            >  2:753747/1‑139 (MQ=255)                                                                                                                               aCTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATAAGTCAGTGCGATGCTTTCAGCAACCt  >  1:1108595/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        TATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCT  >  NC_000913/1121382‑1121646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑