Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,670	C→T	G117G (GGC→GGT)	lolE →	lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,670	0	C	T	100.0%	113.0 / NA	36	G117G (GGC→GGT)	lolE	lipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/15);  total (21/15)

ACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAG  >  NC_000913/1177549‑1177805                                                                                                                          |                                                                                                                                        aCTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGa                                                                                                                        >  2:178208/1‑139 (MQ=255)       aGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCg                                                                                                                  >  2:894433/1‑139 (MQ=255)           aGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTACCGCGCCGTATGTCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTg                                                                                                              <  2:828372/139‑1 (MQ=255)                        gTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCt                                                                                                 >  1:532174/1‑139 (MQ=255)                           cAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCCCGt                                                                                              <  1:894433/139‑1 (MQ=255)                                    ccGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCAGAGCGCATTACCCTCGATTGTCCAgg                                                                                     >  2:960874/1‑139 (MQ=255)                                        gTATTTCCGCTGCCGCGCCGTATATCAATTTCACGCGGCTGGTGGAAAGTGGCGCGCATCTTCGCGCAATCCAGGCGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGa                                                                                 >  2:246362/1‑139 (MQ=255)                                                 ctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg                                                                        <  1:46438/139‑1 (MQ=255)                                                  tgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcgc                                                                       >  2:1289460/1‑139 (MQ=255)                                                  tgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcgc                                                                       <  1:50139/139‑1 (MQ=255)                                                        gcCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCa                                                                                                        >  2:1211635/1‑100 (MQ=255)                                                        gcCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCa                                                                                                        <  1:1211635/100‑1 (MQ=255)                                                            tataTCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAg                                                             <  1:178208/139‑1 (MQ=255)                                                                  aaTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCg                                                       <  1:1289460/139‑1 (MQ=255)                                                                      tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGt                                                                                              >  2:565570/1‑96 (MQ=255)                                                                      tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGt                                                                                              <  1:565570/96‑1 (MQ=255)                                                                      tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACa                                                   >  1:1043294/1‑139 (MQ=255)                                                                      tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACa                                                   >  1:167030/1‑139 (MQ=255)                                                                      tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCC‑CGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAg                                                  >  1:439707/1‑139 (MQ=255)                                                                        aCCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGc                                                 <  2:532174/139‑1 (MQ=255)                                                                        aCCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGc                                                 >  2:421010/1‑139 (MQ=255)                                                                              cTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTa                                           >  2:1230986/1‑139 (MQ=255)                                                                                      aaGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGc                                   >  2:347511/1‑139 (MQ=255)                                                                                       aGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCa                                  >  1:889585/1‑139 (MQ=255)                                                                                        gTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAAccc                                                                                                                                 <  2:1099754/43‑1 (MQ=255)                                                                                        gTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAAccc                                                                                                                                 >  1:1099754/1‑43 (MQ=255)                                                                                         tGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACa                                                                                                                           <  1:489795/48‑1 (MQ=255)                                                                                         tGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACa                                                                                                                           >  2:489795/1‑48 (MQ=255)                                                                                           gcgcGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGa                                                      <  1:1077915/115‑1 (MQ=255)                                                                                           gcgcGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGa                                                      >  2:1077915/1‑115 (MQ=255)                                                                                                    ttCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGa                     <  1:347511/139‑1 (MQ=255)                                                                                                      cgcgCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATg                   <  1:421010/139‑1 (MQ=255)                                                                                                                    gAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAg     <  2:1043294/139‑1 (MQ=255)                                                                                                                       gggTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAg  >  2:387031/1‑139 (MQ=255)                                                                                                                       gggTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAg  >  2:312343/1‑139 (MQ=255)                                                                                                                       gggTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAg  >  1:872975/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                        ACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAG  >  NC_000913/1177549‑1177805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑